Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Mohd Faheem Khan, Cormac D. Murphy
Summary: In this study, a new cytochrome P450 monooxygenase was identified in the fungus Cunninghamella elegans, which is capable of catalyzing the biotransformation of structurally diverse xenobiotic substrates. Co-expression of different CYP reductases improved the production and quality of metabolites. The selection of different reductases is likely to contribute to the broad range of xenobiotic biotransformation observed in C. elegans.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Xu Wang, Jingjing Fa, Yuanjin Zhang, Shengbo Huang, Jie Liu, Junqing Gao, Lina Xing, Zongjun Liu, Xin Wang
Summary: There is a potential interaction between Danshen tablet and rivaroxaban as the former inhibits the metabolism of the latter. This may be due to the strong inhibitory effects of Danshen tablet's components, particularly dihydrotanshinone I, on CYP enzymes. Therefore, drug monitoring and dosage adjustment are necessary when Danshen tablet and rivaroxaban are used simultaneously in clinical practice.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sarah M. Glass, Stephany N. Webb, F. Peter Guengerich
Summary: Among the 57 human cytochrome P450 enzymes, seven are mitochondrial and utilize an electron transport system with adrenodoxin (Adx) and NADPHadrenodoxin reductase (AdR) to mediate protein interactions through electrostatic interactions. Experimental and computational studies have shown that despite some differences in interactions, the binding between P450 and Adx is P450-specific.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Andre Fischer, Martin Smiesko
Summary: This study investigates an allosteric site in Cytochrome P450 enzymes, finding it to be well conserved in the CYP2 family with the exception of CYP2E1, and observing an enlargement of ligand tunnels in certain members that facilitate ligand translocation. The research contributes to understanding ligand access phenomena and aids in estimating the selectivity of metabolic transformations using computational techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Vanessa Rosa Retamoso, Fernanda Barbisan, Graziele Meira Moro, Patricia Maurer, Debora Vasquez Rubio, Lauren Flores Viera dos Santos, Lyana Berro Feijoo, Matias Nunes Frizzo, Ivana Beatrice Manica da Cruz, Vanusa Manfredini, Ana Leticia Vargas Barcelos, Jacqueline da Costa Escobar Piccoli
Summary: This study evaluates the effect of the BsmI single nucleotide polymorphism (SNP) of the VDR gene on the expression of VDR, SOD2, and CYP24A1 genes in individuals with low serum vitamin D levels. The results show that the BsmI SNP of the VDR gene leads to overexpression of the CYP24A1 gene and low expression of the SOD2 gene. Therefore, the BsmI SNP of the VDR gene can modulate gene expression without interfering with serum vitamin D levels.
Article
Biochemistry & Molecular Biology
Jessica A. Gable, Thomas L. Poulos, Alec H. Follmer
Summary: P450lin has higher selectivity for its non-native redox partner Arx than its native partner Pdx, and Pdx shows higher activity when mutated, indicating that the interactions between P450lin and its redox partners are different from P450cam-Pdx.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Hang Yu, Meiqi Song, Keqi Hu, Yujian Wang, Ruifang Fan, Zongying Yang, Hansruedi Glatt, Albert Braeuning, Yungang Liu
Summary: Bisphenol compounds may increase the genotoxic effects of metabolically activated carcinogens by modulating the expression of nuclear receptors and xenobiotic-metabolizing enzymes in human liver cancer cells, potentially aggravating chromosome damage caused by impactful carcinogens in human cells.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Natthaporn Kuendee, Alisa Naladta, Thitianan Kulsirirat, Thunyatorn Yimsoo, Werayut Yingmema, Kanoktip Pansuksan, Korbtham Sathirakul, Sophida Sukprasert
Summary: This study investigated the effects of Lysiphyllum strychnifolium extracts on the gene expression of drug-metabolizing enzymes in rats. The results showed that different doses of the extracts can modulate the mRNA expression levels of clinically relevant CYP genes, and gender also plays a role in gene expression. These findings provide valuable information for the practical use of Lysiphyllum strychnifolium extracts in humans.
Article
Pharmacology & Pharmacy
Fabio Bachmann, Urs Duthaler, Stephan Krahenbuhl
Summary: This study investigated the effect of deglucuronidation on the plasma concentration of the constituents of the Basel phenotyping cocktail. The results showed that deglucuronidation of plasma samples improves the stability of phenotyping results and is necessary to reliably detect CYP induction.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ragini D. D. Singh, Avadhesh Avadhesh, Gaurav Sharma, Sagar Dholariya, Rima B. B. Shah, Bela Goyal, Subash Chandra Gupta
Summary: Targeted cancer therapy using CYP as drug target has shown promise, but there are still challenges including drug resistance and limited therapeutic index. Recent advances in molecular tools and structure-activity relationship understanding have helped in the development of CYP-based drugs with better efficacy. Future directions involve the development of more selective tools to understand molecular alterations and develop specific CYP inhibitors/inducers.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Engineering, Environmental
Hang Yu, Yungang Liu
Summary: Bisphenol compounds are significant environmental pollutants and disruptors of the endocrine system. This study suggests that combined and extended exposure to these compounds can enhance their genotoxicity, specifically promoting the formation of aneuploid chromosomes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Toxicology
Slobodan P. Rendic, F. Peter Guengerich
Summary: This overview discusses the metabolic activation of various compounds by cytochrome P450 enzymes, with a focus on bioactivation reactions. The study identifies a higher level of activation for general chemicals, drugs, and natural products compared to physiological compounds. Enzymes such as P450 3A4, 1A2, 2E1, and 1A1 are highlighted for their roles in catalyzing the formation of activated metabolites from different compound groups.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Plant Sciences
Pramesti Istiandari, Shuhei Yasumoto, Pisanee Srisawat, Keita Tamura, Ayaka Chikugo, Hideyuki Suzuki, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka
Summary: Research reveals that different plant CPR classes have varied effects on triterpenoid production when co-expressed with different CYP classes. Co-expression of these genes in yeast can lead to higher yields and more valuable compounds.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Chemistry, Applied
Sebastian A. Loew, Bettina M. Nestl, Martin J. Weissenborn, Ferdinand Zepeck, Bernhard Hauer
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2015)
Article
Biochemistry & Molecular Biology
Enrique C. Llaudet, Dominique Darimont, Ramona Samba, Ilona Matiychyn, Martin Stelzle, Martin J. Weissenborn, Bernhard Hauer
Article
Chemistry, Multidisciplinary
Martin J. Weissenborn, Sandra Notonier, Sarah-Luise Lang, Konrad B. Otte, Susanne Herter, Nicholas J. Turner, Sabine L. Flitsch, Bernhard Hauer
CHEMICAL COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Martin J. Weissenborn, Damien P. Debecker, Samuel Golten, Bruno Linclau, Nicholas J. Turner, Sabine L. Flitsch
PROTEIN AND PEPTIDE LETTERS
(2017)
Article
Biochemistry & Molecular Biology
Dominique Darimont, Martin J. Weissenborn, Bernd A. Nebel, Bernhard Hauer
BIOELECTROCHEMISTRY
(2018)
Article
Chemistry, Analytical
Rouslan V. Olkhov, Martin J. Weissenborn, Sabine L. Flitsch, Andrew M. Shaw
ANALYTICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Dominic P. H. M. Heuts, Martin J. Weissenborn, Rouslan V. Olkhov, Andrew M. Shaw, Jennet Gummadova, Colin Levy, Nigel S. Scrutton
Article
Biochemistry & Molecular Biology
Sonia Serna, Cornelis H. Hokke, Martin Weissenborn, Sabine Flitsch, Manuel Martin-Lomas, Niels-Christian Reichardt
Review
Chemistry, Multidisciplinary
Christopher J. Gray, Martin J. Weissenborn, Claire E. Eyers, Sabine L. Flitsch
CHEMICAL SOCIETY REVIEWS
(2013)
Article
Chemistry, Organic
Johannes W. Wehner, Martin J. Weissenborn, Mirja Hartmann, Christopher J. Gray, Robert Sardzik, Claire E. Eyers, Sabine L. Flitsch, Thisbe K. Lindhorst
ORGANIC & BIOMOLECULAR CHEMISTRY
(2012)
Article
Chemistry, Physical
Sara M. Hoffmann, Hamid-Reza Danesh-Azari, Claudia Spandolf, Martin J. Weissenborn, Gideon Grogan, Bernhard Hauer
Article
Chemistry, Physical
Martin J. Weissenborn, Sebastian A. Loew, Niels Borlinghaus, Miriam Kuhn, Stefanie Kummer, Fabian Rami, Bernd Plietker, Bernhard Hauer
Article
Chemistry, Physical
Sara M. Hoffmann, Martin J. Weissenborn, Lukasz Gricman, Sandra Notonier, Juergen Pleiss, Bernhard Hauer
Article
Chemistry, Physical
Sebastian A. Loew, Isabell M. Loew, Martin J. Weissenborn, Bernhard Hauer