期刊
ACS SENSORS
卷 1, 期 8, 页码 970-974出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00401
关键词
RNase H; boronic acid; peroxynitrite; ADN; stimuli-responsive
资金
- Agence Nationale de la Recherche [ANR PRODYGY-11-JS07-005-01]
- Fondation pour la Recherche Medicale [DBS 20140930769]
- CNRS
- Region Languedoc-Roussillon (Programme Chercheur d'Avenir)
RNase H is a ubiquitous intracellular enzyme able to selectively hydrolyze the RNA strand of an RNA/DNA hybrid duplex. In the present study, we demonstrate that 5'-boronic acid modified oligonucleotides could inhibit RNase H activity; actvity which could subsequently be restored upon oxidation of the boronic acid moiety. This unprecedented dual property was exploited to engineer a new stimuli-responsive system for the chemoselective imaging of endogenous and exogenous peroxynitrite in RAW264.7 cells.
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