Article
Spectroscopy
Wenlu Wu, Zhongfei Hu, Cai Shi, Ruoqian Xu, Yiming Zhao, Yujie Ding
Summary: A Fe3+ optical sensor was developed by linking CdTe quantum dots and a Rhodamine B derivative using gamma-cyclodextrin as a bridge. The nanoprobe displayed a response to Fe3+ through a fluorescence resonance energy transfer process. The probe showed satisfactory linearity in the fluorescent quenching between 10 and 60 μM Fe3+ concentration, with a limit of detection of 2.51 μM. The probe was successfully used for the determination of Fe3+ in human serum with high sensitivity and exceptional selectivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Mohamad Mahani, Majedeh Faghihi-Fard, Faten Divsar, Masoud Torkzadeh-Mahani, Faeze Khakbaz
Summary: In this study, a label-free and specific FRET-based IL-6 aptasensor was developed. The biosensor showed high sensitivity, excellent selectivity, and could be applied for IL-6 detection in clinical settings.
Article
Materials Science, Multidisciplinary
Amadeo Sena-Torralba, Helena Torne-Morato, Claudio Parolo, Saba Ranjbar, Mohammad Amin Farahmand Nejad, Ruslan Alvarez-Diduk, Andrea Idili, Mohammad Reza Hormozi-Nezhad, Arben Merkoci
Summary: The majority of lateral flow assays use single-color labels, but this method has two limitations: it is prone to misinterpretation and does not allow precise modulation of sensitivity and dynamic range. To overcome these limitations, a ratiometric approach is developed that allows for adjustment of the assay's analytical properties.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Dongquan Leng, Jihao Zhao, Xiang Ren, Rui Xu, Lei Liu, Xuejing Liu, Yuyang Li, Qin Wei
Summary: In this study, a split-type immunoassay strategy initiated by cation exchange and altering the capacity of an electron donor was optimized, using MoSe2/CdSe as a photoelectrode substrate and silver ions as the initiator of CE. The combination of ascorbate oxidase with MSNs improved the sensor's sensitivity and reliability, resulting in a broad linear range and low detection limit when applied to neuron-specific enolase.
ANALYTICAL CHEMISTRY
(2021)
Review
Optics
Nikita Toropov, Gema Cabello, Mariana P. Serrano, Rithvik R. Gutha, Matias Rafti, Frank Vollmer
Summary: This review discusses the recent advances in biosensing using microlasers based on whispering-gallery modes (WGMs). These miniature lasers have been successfully applied in label-free detection of single virus particles and live-cell tagging and tracking, showing promising potential in the field of biological sensing.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Umar Nishan, Irfan Ullah, Rukhsana Gul, Amir Badshah, Nawshad Muhammad, Naeem Khan, Mohibullah Shah, Muhammad Asad, Saifullah Afridi, Riaz Ullah, Essam A. Ali, Suvash Chandra Ojha
Summary: Ascorbic acid is vital for human beings and its abnormal level can lead to various illnesses. In this study, silver nanoparticles were synthesized and used as a sensing platform for the detection of ascorbic acid in blood serum samples. The optimized biosensor showed sensitivity and simplicity for the detection of ascorbic acid in plasma samples.
Article
Chemistry, Multidisciplinary
Ruifang Su, Yu-Tang Wu, Sofia Doulkeridou, Xue Qiu, Thomas Just Sorensen, Kimihiro Susumu, Igor L. Medintz, Paul M. P. van Bergen en Henegouwen, Niko Hildebrandt
Summary: This study demonstrates a biosensing approach that combines nanobodies with nanoparticles, specifically attaching nanobodies with different C-terminal tags to widely used biocompatible semiconductor quantum dots. A rapid and wash-free immunoassay for the epidermal growth factor receptor (EGFR) was designed and implemented by displacing Tb-labeled hexahistidine-tagged nanobodies from the QD surfaces via EGFR-nanobody binding, leading to a concentration-dependent decrease in the Tb-to-QD FRET signal. The detection limit of this assay was 80 +/- 20 pM, which is 10-fold lower than conventional sandwich FRET assays.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Spectroscopy
Anmol Arora, Kriti Sharma, S. K. Tripathi
Summary: This study investigated the impact of luminescent MoSe2 quantum dots on trypsin activity and found that MoSe2 quantum dots formed a stable complex with trypsin through electrostatic interactions. This finding is significant for the development of trypsin detection methods and understanding the bioavailability of MoSe2 quantum dots in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Andre Shamsabadi, Tabasom Haghighi, Sara Carvalho, Leah C. C. Frenette, Molly M. M. Stevens
Summary: Nanozymes are nanosized materials that have catalytic properties similar to biological enzymes, making them promising candidates for clinical sensing devices. They can amplify signals in nanosensor-based platforms and improve sensor detection limits. Recent developments have led to highly effective nanozymes that can detect clinically relevant biomarkers, but there are hurdles to overcome before they can be used in clinical diagnostics. This overview discusses the current understanding of nanozymes for disease diagnostics and biosensing applications, and the challenges that need to be addressed for their translation into clinical tests.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Hong Ruan, Li Zhou
Summary: The rapid industrialization has caused serious environmental problems and increased disease and healthcare issues. The development of simple and effective biosensors is crucial for disease prevention and treatment. Sulfur quantum dots, as a new fluorescent nanomaterial, offer great potential in the fields of bioimaging and biosensing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Moritz Schueller, Annette Meister, Mark Green, Lea Ann Dailey
Summary: Combining different CPs can enhance the sensitivity of fluorescent signal transducers, increasing the detection limit of assays like ELISA and LFIs. Encapsulating a polymer blend of PDOF and CN-PPV in Pdots can further increase SBR.
Article
Chemistry, Multidisciplinary
Cheng Yang, Hao Xie
Summary: This study demonstrates the interactions between carbon dots (CDs) and biomolecules through surface modification. Various fluorescence response behaviors of modified CDs were observed, with electrostatic attraction and hydrogen bonding playing important roles in inducing aggregation and fluorescence enhancement of CDs. The study also suggests the occurrence of an inner filter effect and selective antibacterial activity of modified CDs towards Gram-positive bacteria. This work provides potential for developing CD-based techniques for detecting and visualizing proteins/bacteria, as well as selective antibacterial agents.
Article
Chemistry, Applied
Yuyan Zhu, Guojing Li, Wei Li, Xiaogang Luo, Zhiyuan Hu, Fengshou Wu
Summary: Intense red-emissive carbon quantum dots (r-CQDs) were synthesized through one-pot hydrothermal treatment of methylene violet. The r-CQDs showed high solubility in water and exhibited excitation-independent emission with a maximum fluorescence peak at 596 nm. They could be used as fluorescence sensors for detecting trace amounts of water in organic solvents and showed selective response to hypochlorite. The good biocompatibility of r-CQDs was verified and they were successfully used in cellular imaging, mainly located in lysosomes.
Review
Chemistry, Multidisciplinary
G. Prabakaran, K. Velmurugan, C. Immanuel David, R. Nandhakumar
Summary: Fluorescence sensing based on Forster Resonance Energy Transfer (FRET) using graphene-based nanomaterials has gained significant interest in various fields. Graphene quantum dots (GQDs), graphene oxide (GO), reduced graphene oxide (rGO), and graphdiyne (GD) can be used as donors/acceptors in FRET-based sensing due to their unique properties. This review discusses the basics of FRET and the role of graphene-based nanomaterials in sensing various analytes, as well as explaining the lower detection ranges. Challenges and future directions for designing nanomaterials with improved sensing performance are also highlighted.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Shipeng Wang, Haoran Zhao, Jinliang Yang, Yahui Dong, Shaozheng Guo, Qian Cheng, Yu Li, Shouxin Liu
Summary: Multicolor fluorescent biomass carbon dots (BCDs) were prepared from spinach using an ecofriendly solvothermal method. The luminescence colors of the obtained BCDs can be tuned by changing the solvents, which affects the carbonization process of polysaccharides and chlorophyll in spinach. The blue BCDs show excellent sensitivity and selectivity towards Cr(VI), making them a promising candidate for ion detection.
Article
Biochemical Research Methods
Giacomo Annio, Travis L. Jennings, Oya Tagit, Niko Hildebrandt
BIOCONJUGATE CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Paula de Almeida, Maarten Jaspers, Sarah Vaessen, Oya Tagit, Giuseppe Portale, Alan E. Rowan, Paul H. J. Kouwer
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Physical
Erica D. de Avila, Antonio G. B. Castro, Oya Tagit, Bastiaan P. Krom, Dennis Lowik, Ad A. van Well, Lars J. Bannenberg, Carlos Eduardo Vergani, Jeroen J. J. P. van den Beucken
APPLIED SURFACE SCIENCE
(2019)
Article
Multidisciplinary Sciences
Robin Ferrari, Gaelle Martin, Oya Tagit, Alan Guichard, Alessandra Cambi, Raphael Voituriez, Stephane Vassilopoulos, Philippe Chavrier
NATURE COMMUNICATIONS
(2019)
Article
Pharmacology & Pharmacy
Maria Camilla Operti, Yusuf Dolen, Jibbe Keulen, Eric A. W. van Dinther, Carl G. Figdor, Oya Tagit
Article
Engineering, Biomedical
Alexander H. J. Staal, Katrin Becker, Oya Tagit, N. Koen van Riessen, Olga Koshkina, Andor Veltien, Pascal Bouvain, Kimberley R. G. Cortenbach, Tom Scheenen, Ulrich Floegel, Sebastian Temme, Mangala Srinivas
Article
Chemistry, Applied
Beatriz S. Verza, Jeroen J. J. P. van den Beucken, Joao Brandt, Miguel Jafelicci Junior, Valentim A. R. Barao, Rodolfo D. Piazza, Oya Tagit, Denise M. P. Spolidorio, Carlos Eduardo Vergani, Erica D. de Avila
Summary: This study developed a drug delivery system using anionic beta cyclodextrin complexes to retain and control the release of tetracycline on titanium surface. The system showed controlled drug release over 15 days, sustained release over 30 days, and antibacterial activity against Staphylococcus aureus. The non-cytotoxic effect on human gingival fibroblast growth was also demonstrated.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemical Research Methods
Massis Krekorian, Gerwin G. W. Sandker, Kimberley R. G. Cortenbach, Oya Tagit, N. Koen van Riessen, Rene Raave, Mangala Srinivas, Carl G. Figdor, Sandra Heskamp, Erik H. J. G. Aarntzen
Summary: The study demonstrates the development of PLGA-NH2 NPs for ex vivo cell labeling and in vivo cell tracking using SPECT. These NPs show high specific activity for cell labeling without affecting cell viability, and successfully track ex vivo labeled cells in an infection model in mice.
BIOCONJUGATE CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Maria Camilla Operti, Alexander Bernhardt, Silko Grimm, Andrea Engel, Carl Gustav Figdor, Oya Tagit
Summary: Nanomedicines based on PLGA carriers show great potential for biomedical research, but are not yet available on the global market. Challenges include scaling up production and successful translation into clinical practice, requiring advancements in manufacturing techniques and quality control methods.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Maria Camilla Operti, Alexander Bernhardt, Vladimir Sincari, Eliezer Jager, Silko Grimm, Andrea Engel, Martin Hruby, Carl Gustav Figdor, Oya Tagit
Summary: Despite the therapeutic benefits of poly(lactic-co-glycolic acid) (PLGA) nanomedicine formulations, large-scale processing challenges hinder their clinical and commercial development. This study presents the development of an inline sonication process for the industrial-scale production of PLGA nanomedicines, along with optimized downstream processes for purification, concentration, and storage. The developed manufacturing and processing technologies enable the continuous and efficient production of PLGA-based nanomedicines.
Article
Pharmacology & Pharmacy
Maria Camilla Operti, Alexander Bernhardt, Jeanette Pots, Vladimir Sincari, Eliezer Jager, Silko Grimm, Andrea Engel, Anne Benedikt, Martin Hruby, Ingrid Jolanda M. De Vries, Carl G. Figdor, Oya Tagit
Summary: A reliable manufacturing technique using an inline sonication process was developed for the industrial scale production of immunomodulating PLGA nanovaccines, successfully replicating the properties and functions of laboratory-scale nanovaccines. This inline sonication method is suitable for the continuous scale-up production of nanomedicine formulations, demonstrating good efficacy and safety.
Article
Chemistry, Multidisciplinary
Cian Holohan, Sophia Hanrahan, Nathan Feely, Peng Li, John O'Connell, Catherine Moss, Michael Carr, Oya Tagit, Gil U. Lee
Summary: The study found that the choice of viral transport media and freeze-thaw cycles significantly impact the sensitivity of COVID-19 molecular diagnostics assays, affecting the stability of the SARS-CoV-2 viral genome. Testing with glycerol-containing PBS is recommended for high stability and sensitivity, as supported by clinical studies on patient-derived samples.
Article
Multidisciplinary Sciences
Laia Querol Cano, Oya Tagit, Yusuf Dolen, Anne van Duffelen, Shannon Dieltjes, Sonja Buschow, Toshiro Niki, Mitsuomi Hirashima, Ben Joosten, Koen van den Dries, Alessandra Cambi, Carl G. Figdor, Annemiek B. van Spriel
Article
Nanoscience & Nanotechnology
Edyta Swider, Sanish Maharjan, Karlijne Houkes, Nicolaas Koen van Riessen, Carl Figdor, Mangala Srinivas, Oya Tagit
ACS APPLIED BIO MATERIALS
(2019)
Article
Oncology
Yusuf Dolen, Michael Valente, Oya Tagit, Eliezer Jager, Eric A. W. Van Dinther, N. Koen van Riessen, Martin Hruby, Uzi Gileadi, Vincenzo Cerundolo, Carl G. Figdor
