Article
Neurosciences
Adam S. Ravalia, James Lau, Jessica C. Barron, Stephanie L. M. Purchase, Amber L. Southwell, Michael R. Hayden, Firoozeh Nafar, Matthew P. Parsons
Summary: Synaptic structure and function are compromised in the presymptomatic stage of Huntington's disease, with increased AMPA receptor subunit clustering and hyperexcitability observed in the hippocampus. These synaptic abnormalities are not exclusive to the striatum, highlighting the need to better understand region-dependent complexities of early synaptopathy in the HD brain.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Neurosciences
Anna Bodzeta, Nicky Scheefhals, Harold D. MacGillavry
Summary: The various functions of glutamate in the brain are mediated by ionotropic and metabotropic glutamate receptors, which modulate synaptic transmission and plasticity. mGluRs play roles at both presynaptic and postsynaptic sites, acting as essential signal integrators that couple mechanisms of transmission and plasticity.
Article
Multidisciplinary Sciences
Yicong Wu, Xiaofei Han, Yijun Su, Melissa Glidewell, Jonathan S. Daniels, Jiamin Liu, Titas Sengupta, Ivan Rey-Suarez, Robert Fischer, Akshay Patel, Christian Combs, Junhui Sun, Xufeng Wu, Ryan Christensen, Corey Smith, Lingyu Bao, Yilun Sun, Leighton H. Duncan, Jiji Chen, Yves Pommier, Yun-Bo Shi, Elizabeth Murphy, Sougata Roy, Arpita Upadhyaya, Daniel Colon-Ramos, Patrick La Riviere, Hari Shroff
Summary: Researchers have enhanced the performance of confocal microscopy using four approaches: developing compact line scanners, combining multi-view imaging, adapting techniques from structured illumination microscopy, and utilizing deep learning. They successfully improved imaging resolution, reduced phototoxicity, and demonstrated these advancements on over 20 different samples.
Review
Neurosciences
Jeremy M. Henley, Jithin D. Nair, Richard Seager, Busra P. Yucel, Gavin Woodhall, Benjamin S. Henley, Karolina Talandyte, Hope I. Needs, Kevin A. Wilkinson
Summary: Epilepsy is caused by abnormal neuronal network activity. The review focuses on the role and regulation of kainate and AMPA receptors in seizure activity, discussing the impact of GluK2 and GluA2 subunits on their respective receptors' properties.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Optics
Mingwei Tang, Yubing Han, Danchen Jia, Qing Yang, Ji-Xin Cheng
Summary: Far-field chemical microscopy, with molecular electronic or vibrational fingerprint information, offers a new perspective for studying three-dimensional biological, material, and chemical systems. Despite the limitation of optics, recent advances in super-resolution techniques have overcome this hurdle, pushing the boundaries of far-field chemical microscopy in terms of spatial resolution. This technique finds applications in various fields including biomedical research, material characterization, environmental study, cultural heritage conservation, and integrated chip inspection.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Hongru Li, Jinrui Zhang, Yan Shi, Guanfang Zhao, Haijiao Xu, Mingjun Cai, Jing Gao, Hongda Wang
Summary: This study uses super-resolution imaging to reveal the distribution changes of the insulin receptor during cell membrane activation. The findings show that insulin resistance inhibits receptor clustering and there is a highly co-localized relationship between the insulin receptor and the skeletal protein beta II-spectrin.
Article
Biochemical Research Methods
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk, Cara R. Schiavon, Seungyoon B. Yu, Tong Zhang, Melissa Wu, Kyle Kastner, Alaa Abdel Latif, Zijun Lin, Andrew Shaw, Yoshiyuki Kubota, John Mendenhall, Zhao Zhang, Gulcin Pekkurnaz, Kristen Harris, Jeremy Howard, Uri Manor
Summary: Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Article
Biochemical Research Methods
Pierre Parutto, Jennifer Heck, Meng Lu, Clemens Kaminski, Edward Avezov, Martin Heine, David Holcman
Summary: This study presents data analysis and algorithms applicable to super-resolution imaging, which can extract quantitative information from massive datasets, reconstruct subcellular structures and dynamics, and measure disease-related changes.
CELL REPORTS METHODS
(2022)
Article
Chemistry, Physical
Karine Queiroz Zetune Villa Real, Nikolaos Mougios, Ronja Rehm, Shama Sograte-Idrissi, Laszlo Albert, Amir Mohammad Rahimi, Manuel Maidorn, Jannik Hentze, Markel Martinez-Carranza, Hassan Hosseini, Kim-Ann Saal, Nazar Oleksiievets, Matthias Prigge, Roman Tsukanov, Pal Stenmark, Eugenio F. Fornasiero, Felipe Opazo
Summary: Living synapse imaging has traditionally relied on fluorescently tagged synaptic proteins, but this approach can alter synapse physiology. To overcome these limitations, a noninvasive nanobody (iNbSyt1) that binds to synaptotagmin-1 has been developed. This nanobody allows for the generation of protein-based fluorescent reporters and is ideal for super-resolution imaging methods. Overall, nanobody NbSyt1 enables precise imaging in cellular and molecular neuroscience across multiple spatiotemporal scales.
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
Review
Anatomy & Morphology
Yutaro Kashiwagi, Shigeo Okabe
Summary: Neuronal circuits in the neocortex and hippocampus play a crucial role in higher brain functions and are regulated by spine synapses. Advances in super-resolution microscopy have revealed the molecular mechanisms of nanoscale synaptic structures in living neurons, which have significant implications for synaptic function, learning, and memory. Spine size and morphology are closely related to synaptic transmission efficacy and signal transduction, contributing to the cellular basis of cognition.
ANATOMICAL SCIENCE INTERNATIONAL
(2021)
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: The technique surpasses the resolving power of multi-million-dollar machines.
Article
Biochemistry & Molecular Biology
Ivona Kubalova, Alzbeta Nemeckova, Klaus Weisshart, Eva Hribova, Veit Schubert
Summary: Fluorescence light microscopy is important for studying cellular structures, but limited by light diffraction; super-resolution techniques like SIM are used to overcome this limitation; however, STED and SMLM, which offer higher resolution, are not widely applied in plant cell research due to specific sample preparation requirements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jing Gao, Hongru Li, Haijiao Xu, Yong Liu, Mingjun Cai, Yan Shi, Jingrui Zhang, Hongda Wang
Summary: Using dSTORM imaging, nanoscale GCGR clusters were observed on HepG2 cell membranes, and high glucose promoted GCGR expression and the formation of more and larger clusters. Glucagon stimulation under high glucose did not inhibit GCGR levels as significantly as that under low glucose, and did not increase the downstream cAMP-PKA signal, indicating high glucose-induced glucagon resistance. Furthermore, high glucose induced stronger glucagon resistance in hepatoma cells compared with hepatic cells.
Correction
Biology
Kalina T. Haas, Benjamin Compans, Mathieu Letellier, Thomas M. Bartol, Dolors Grillo-Bosch, Terrence J. Sejnowski, Matthieu Sainlos, Daniel Choquet, Olivier Thoumine, Eric Hosy
Review
Neurosciences
Daniel Choquet, Matthieu Sainlos, Jean-Baptiste Sibarita
Summary: The brain is a highly complex organ, and advancements in imaging methods and molecular tools have revolutionized our understanding of its structure and function. By combining the latest molecular tools with imaging techniques, researchers are able to investigate neural function at a greater resolution than previously possible.
NATURE REVIEWS NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Luis F. Ribeiro, Tatiana Catarino, Mario Carvalho, Luisa Cortes, Sandra D. Santos, Patricio O. Opazo, Lyn Rosenbrier Ribeiro, Barbara Oliveiros, Daniel Choquet, Jose A. Esteban, Joao Peca, Ana Luisa Carvalho
Summary: Research has shown that the high ligand-independent activity of the ghrelin receptor plays an important role in the physiology of excitatory synapses in the hippocampus, affecting the surface diffusion and phosphorylation of AMPA receptors. Blocking this activity not only affects the AMPA receptor-dependent synaptic delivery induced by chemical long-term potentiation, but also impairs spatial and recognition memory in mice.
Article
Neurosciences
Tomohisa Hosokawa, Pin-Wu Liu, Qixu Cai, Joana S. Ferreira, Florian Levet, Corey Butler, Jean-Baptiste Sibarita, Daniel Choquet, Laurent Groc, Eric Hosy, Mingjie Zhang, Yasunori Hayashi
Summary: The authors demonstrate that CaMKII undergoes liquid-liquid phase separation triggered by calcium signaling, which leads to the reorganization of postsynaptic structures and potentially enhances synaptic transmission efficacy during memory formation. This process of phase separation, induced by calcium signals, may serve as an activity-dependent mechanism to crosslink postsynaptic proteins, contributing to synaptic reorganization associated with synaptic plasticity.
NATURE NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Benjamin Compans, Come Camus, Emmanouela Kallergi, Silvia Sposini, Magalie Martineau, Corey Butler, Adel Kechkar, Remco Klaassen, Natacha Retailleau, Terrence J. Sejnowski, August B. Smit, Jean-Baptiste Sibarita, Thomas M. Bartol, David Perrais, Vassiliki Nikoletopoulou, Daniel Choquet, Eric Hosy
Summary: Long-term depression (LTD) of synaptic strength is associated with circuit remodeling, memory encoding, and erasure. P2XR- and NMDAR-dependent LTD are linked to distinct molecular modifications that lead to specific changes in synapse function.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
May Bakr, Damien Jullie, Julia Krapivkina, Vincent Paget-Blanc, Lou Bouit, Jennifer D. Petersen, Natacha Retailleau, Christelle Breillat, Etienne Herzog, Daniel Choquet, David Perrais
Summary: The study identifies VAMP4 as the vesicular SNARE that mediates most dendritic recycling endosome exocytosis, while VAMP2 plays a minor role in this process. Knock down of VAMP4 reduces TfR recycling but increases AMPAR recycling, leading to increased AMPAR-mediated synaptic transmission and occlusion of LTP expression. The opposing changes in AMPAR and TfR recycling upon VAMP4 knock down reveal their sorting into separate endosomal populations.
Review
Neurosciences
Hanna L. Zieger, Daniel Choquet
Summary: Neurodevelopmental disorders may originate from genetic defects in synaptic proteins, disrupting the precise nanoscale organization of synapses and impacting synaptic function.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Cell Biology
Daniel Choquet, Patricio Opazo
Summary: This article reviews the evidence supporting the role of AMPAR lateral diffusion in limiting the rate of LTP and its importance in learning and memory. The authors propose multiple solutions for achieving the diffusion trapping of AMPAR during LTP, mainly through interactions with other proteins. The role of AMPAR lateral diffusion not only provides a conceptual leap in our understanding of memory, but also holds potential as a therapeutic target for memory-related disorders.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Emmanouela Kallergi, Akrivi-Dimitra Daskalaki, Angeliki Kolaxi, Come Camus, Evangelia Ioannou, Valentina Mercaldo, Per Haberkant, Frank Stein, Kyriaki Sidiropoulou, Yannis Dalezios, Mikhail M. Savitski, Claudia Bagni, Daniel Choquet, Eric Hosy, Vassiliki Nikoletopoulou
Summary: Autophagy is required for pruning dendritic spines and long-term depression (LTD), a major form of synaptic plasticity. LTD induces the biogenesis of autophagic vesicles in dendrites to facilitate the degradation of postsynaptic proteins. Autophagy plays a crucial role in dendritic spine pruning and synaptic plasticity.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Diogo Bessa-Neto, Gerti Beliu, Alexander Kuhlemann, Valeria Pecoraro, Soren Doose, Natacha Retailleau, Nicolas Chevrier, David Perrais, Markus Sauer, Daniel Choquet
Summary: Advancements in biological imaging are closely related to developments in labeling methods, particularly with the need for new approaches with the rise of high-resolution and super-resolution imaging techniques. The authors successfully developed a labeling and imaging platform using genetic code expansion and non-canonical amino acids to fluorescently label live neurons and localize target proteins with super resolution microscopy, overcoming the limitations of existing imaging tools in visualizing protein localization.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Andrea Toledo, Mathieu Letellier, Giorgia Bimbi, Beatrice Tessier, Sophie Daburon, Alexandre Favereaux, Ingrid Chamma, Kristel Vennekens, Jeroen Vanderlinden, Matthieu Sainlos, Joris De Wit, Daniel Choquet, Olivier Thoumine
Summary: This study reveals the mechanism of interaction between MDGA and neuroligins and shows that MDGA can delay the assembly of functional excitatory synapses containing AMPA receptors.
Article
Multidisciplinary Sciences
Angela M. Getz, Mathieu Ducros, Christelle Breillat, Aurelie Lampin-Saint-Amaux, Sophie Daburon, Urielle Francois, Agata Nowacka, Monica Fernandez-Monreal, Eric Hosy, Frederic Lanore, Hanna L. Zieger, Matthieu Sainlos, Yann Humeau, Daniel Choquet
Summary: This study presents a new method to track and modify synaptic neurotransmitter receptor content using biotin acceptor peptide (AP) tagging. By generating knock-in mice expressing GluA2 receptors with AP tags, it becomes possible to control receptor mobility and achieve target-specific control of synaptic plasticity and animal behavior.
Article
Biochemical Research Methods
Maxime Malivert, Fabrice Harms, Cynthia Veilly, Jerome Legrand, Ziqiang Li, Emmanuelle Bayer, Daniel Choquet, Mathieu Ducros
Summary: Lattice light-sheet microscopy (LLSM) is an efficient technique for high resolution 3D imaging of dynamic phenomena in biological samples. However, optical aberrations caused by refractive index mismatch limit the depth of LLSM imaging. To address this issue, we propose a simple and low-cost active image optimization (AIO) method to recover high resolution imaging in thick biological samples.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemistry & Molecular Biology
Asuncion Carmona, Si Chen, Florelle Domart, Daniel Choquet, Richard Ortega
Summary: Neurons form growth cones during neurodevelopment to establish synaptic connections, and the distributions of F-actin and various biologically active elements in the growth cones play important roles. Calcium is crucial in this process, but other elements are also involved. Deficiencies in these elements, such as iron or zinc, may lead to learning and memory deficits in children.