期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 10, 页码 1817-1826出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00031
关键词
bioprinting; tissue modeling; preeclampsia; placenta; trophoblast; migration; tissue engineering; biomaterial; shear wave elastography
资金
- Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, Washington, DC
Preeclampsia (PE) is a leading cause of maternal and perinatal morbidity and mortality. Current research suggests that the impaired trophoblastic invasion of maternal spiral arteries contributes significantly to the development of PE. However, the pathobiology of PE remains poorly understood, and there is a lack of treatment options largely due to ineffective experimental models. Utilizing the capability of bioprinting and shear wave elastography, we developed a 3D, bioengineered placenta model (BPM) to study and quantify cell migration. Through BPM, we evaluated the effect of epidermal growth factor (EGF) on the migratory behavior of trophoblast and human mesenchymal stem cells. Our results demonstrate a positive correlation between cell migration rates and EGF concentration. These results indicate that a feasible ex vivo placental model can be bioprinted to examine cellular, molecular, and pharmacologic interactions. In addition, EGF clearly affects the celluar migration, a potential therapeutic agent to treat preeclampsia. We envision that our ex vivo tissue modeling approach can be readily transferred to study other normal biologic and abnormal pathologic processes such as fibroblast migration in wound healing and stem cell homing.
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