期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 11, 页码 1983-1991出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00390
关键词
molecular dynamics simulations; graphene quantum dots; cytotoxicity; lipid membrane; membrane disruption
资金
- National Natural Science Foundation of China [21503186, 21403049, 21674032, 61602142]
- Zhejiang Provincial Natural Science Foundation of China [LY13F040006, LY14B030008, LY15E030009]
- Science and Technology Project of Zhejiang Province [2014C33220]
Owing to unique morphology, ultrasmall lateral sizes, and exceptional properties, graphene quantum dots (GQDs) hold great potential in many applications, especially in the field of electrochemical biosensors, bioimaging, drug delivery, et cetera. Its biosafety and potential cytotoxicity to human and animal cells has been a growing concern in recent years. In this work, the potential cytotoxicity of GQDs was evaluated by molecular dynamics simulations. Our simulation demonstrates that small size GQDs could easily permeate into the lipid membrane in a vertical way. It is relatively difficult to permeate into the lipid membrane for GQDs that are larger than GQD61 on the nanosecond time-scale. The thickness of the POPC membrane could even be affected by the small size of GQDs. Free energy calculations revealed that the free energy barrier of GQD permeation through the lipid membrane could greatly change with the change of GQD size. Under high GQD concentration, the GQD molecules could rapidly aggregate in water but disaggregate after entering into the membrane interior. Moreover, high concentrations of GQDs could induce changes in the structure properties and diffusion properties of the lipid bilayer, and it may affect the cell signal transduction. However, GQDs with relatively small size are not large enough to mechanically damage the lipid membrane. Our results suggest that the cytotoxicity of GQDs with small size is low and may be appropriate for biomedical application.
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