期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 12, 页码 2240-2254出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.6b00470
关键词
alginate; bioactive glass; hydrogel; scaffold; freeze-drying; bone tissue engineering
资金
- German Academic Exchange Service (DAAD)
The combination of biodegradable polymers and bioactive inorganic materials is being widely used for designing bone tissue engineering scaffolds. Here we report a composite hydrogel system composed of bioactive glass incorporated in covalently cross-linked oxidized alginate-gelatin hydrogel (ADA-GEL) for designing porous scaffolds with tunable stiffness and degradability using freeze-drying technique. Because of the presence of bioactive glass, the cross-linking kinetic and cross-linking degree of the hydrogels are significantly increased, which is the main factor for the measured enhanced mechanical strength of the bioactive glass containing ADA-GEL scaffolds. The hydrogels with high cross linking degree exhibit low protein release profile and low degradability. Apatite formation on bioactive glass containing hydrogel-based scaffolds is confirmed by FTIR Bone marrow-derived stromal cell growth is promoted in pristine ADA-GEL and 1% bioactive glass containing ADA-GEL scaffolds compared to the scaffolds of pure alginate, alginate gelatin blended hydrogel, and 5% bioactive glass containing ADA-GEL. Initial studies indicated that the scaffolds, especially without bioactive glass, support osteogenic differentiation of murine bone marrow stromal cell line in the absence of foreign osteogenic stimulating supplements; however, they exhibit low levels of osteogenic expression.
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