Article
Engineering, Biomedical
Lior Debbi, Barak Zohar, Margarita Shuhmaher, Yulia Shandalov, Idit Goldfracht, Shulamit Levenberg
Summary: By combining living cells, biological hydrogel, and biodegradable synthetic polymer, a multi-scale vascular network within thick, implantable engineered tissues was successfully fabricated in this study. The functionality and patency of the multi-scale vascular network were demonstrated by dextran passage and physiological flow conditions, leading to significant improvements in blood perfusion compared to control micro-scale-vascularized grafts. This innovative approach of designing and fabricating multi-scale vascular architectures within 3D engineered tissues shows promise for both in vitro models and therapeutic translation research.
Review
Engineering, Biomedical
Mohammad Hadi Norahan, Sara Cristina Pedroza-Gonz, Monica Gabriela Sanchez-Salazar, Mario Moises Alvarez, Grissel Trujillo de Santiago
Summary: Chronic wounds have become a major problem in healthcare systems, leading to death worldwide. Engineering wound dressings with hydrogels can significantly improve healing time, reduce infections, and lower costs. Hydrogels provide a protective barrier, mimic the extracellular matrix, and create a moist environment. Different fabrication methods and properties of hydrogels have been extensively studied for wound healing applications, including the use of additives to enhance tissue regeneration. Hydrogel engineering offers smart solutions for effective wound healing, with capabilities such as inhibiting bacterial growth, promoting re-epithelialization, and improving tissue functionality.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Qingli Liu, Wenling Dai, Yongli Gao, Longpeng Dong, Hengxing Jia, Shikui Li, Likun Guo, Yujiang Fan, Xingdong Zhang
Summary: The physicochemical properties of collagen-based hydrogels and co-culture system have synergistic effects on cartilage formation. The spreading and proliferation of cells are regulated by the degradation rate and stiffness of hydrogels. Co-culture cells in collagen-based hydrogels significantly improve chondrogenesis.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Mattia Vitale, Cosimo Ligorio, Bethan McAvan, Nigel W. Hodson, Chris Allan, Stephen M. Richardson, Judith A. Hoyland, Jordi Bella
Summary: Hydrogels have great potential for tissue engineering applications, but their use in bone regeneration is often hindered by poor mineralization and mechanical properties. Osteoclasts (OCs), which play a pivotal role in bone homeostasis, are often overlooked. This study aims to develop customized, reinforced hydrogels as a platform for studying OC function and differentiation.
ACTA BIOMATERIALIA
(2022)
Article
Biotechnology & Applied Microbiology
Ruimin Long, Linrong Shi, Peng He, Jumei Tian, Shibin Wang, Jun Zheng
Summary: Using hydrogel as a filling medium to recombine dispersed microencapsulated cells and form an embedded gel-cell microcapsule complex is a novel approach for tissue construction. In this experiment, researchers used sodium alginate, chitosan, and silk fibroin to prepare different materials for the complex, and investigated the tissue-forming ability of the complex under different culture conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Vardhman Kumar, David Kingsley, Sajeeshkumar Madhurakkat Perikamana, Pankaj Mogha, C. Rory Goodwin, Shyni Varghese
Summary: This study presents a microfluidic-assisted tissue model of nociception with microvasculature. The neurons and endothelial cells displayed distinct morphologies in the presence of each other. The neurons exhibited an elevated response to capsaicin in the presence of vasculature, and increased TRPV1 receptor expression was observed in the neurons. The platform could also be used to study pain associated with tissue acidosis and pave the way for the development of innervated microphysiological models.
Article
Biochemistry & Molecular Biology
Meltem Guzelgulgen, Dilce Ozkendir-Inanc, Umit Hakan Yildiz, Ahu Arslan-Yildiz
Summary: This study highlights the potential use of Glucuron-oxylan-based quince seed hydrogel as a tissue-engineering scaffold, with optimized gelation, structural characterization, and biocompatibility demonstrated for 3D cell culture and tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
David Grijalva Garces, Carsten Philipp Radtke, Juergen Hubbuch
Summary: A novel strategy was developed to process gelatin at room temperature using urea, which enabled the production of gelatin-methacryloyl derivatives (GelMA). The degree of functionalization of GelMA was controlled by the ratio of reactant volume to gelatin concentration. Hydrogels with tailored mechanical properties were produced by varying GelMA concentration and degree of functionalization. The biocompatibility of the hydrogels formulated with GelMA produced by this method was comparable to those produced by the conventional method.
Article
Engineering, Multidisciplinary
Hieu M. Pham, Yuli Zhang, Jose G. Munguia-Lopez, Simon D. Tran
Summary: Saliva production by salivary glands is essential for oral health. This study investigates the use of Egg White Alginate (EWA) as an alternative scaffold for culturing salivary gland cells and shows that EWA can support cell viability and promote larger spheroid formation compared to Matrigel (R).
Article
Engineering, Biomedical
Pascal Bertsch, Lea Andree, Negar Hassani Besheli, Sander C. G. Leeuwenburgh
Summary: This study investigates the stress relaxation of colloidal hydrogels assembled from gelatin nanoparticles and compares them to physical gelatin hydrogels. Colloidal hydrogels exhibit a strain-dependent fluidization at strains relevant for cell activity, offering the possibility to modulate viscoelasticity and obtain fast stress relaxation rates. These beneficial features make colloidal hydrogels promising alternatives to conventional monolithic hydrogels for tissue engineering and regenerative medicine.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Ximin Yuan, Zhou Zhu, Pengcheng Xia, Zhenjia Wang, Xiao Zhao, Xiao Jiang, Tianming Wang, Qing Gao, Jie Xu, Debin Shan, Bin Guo, Qingqiang Yao, Yong He
Summary: Tough hydrogel is difficult to be used for tissue regeneration due to its poor biocompatibility, nondegradation, and compositional differences from natural tissues. This study proposes a gelatin-based tough hydrogel (GBTH) that overcomes these limitations. GBTH exhibits excellent tensile strength and can be sutured to ruptured tendons, promoting tendon differentiation and allowing quick return to the initial state within eight weeks. This strategy provides a new avenue for highly biocompatible tough hydrogel preparation for tissue regeneration.
Article
Materials Science, Paper & Wood
Elaheh Dalir Abdolahinia, Mahdieh Alipour, Marziyeh Aghazadeh, Mehdi Hassanpour, Marjan Ghorbani, Zahra Aghazadeh
Summary: The complex anatomy of teeth hinders regenerative treatments. This study developed an injectable hydrogel containing MTA and evaluated its physicochemical properties. The hydrogel promoted proliferation and differentiation of human dental pulp stem cells, suggesting its potential as a scaffold for regenerative endodontic procedures.
Review
Biochemistry & Molecular Biology
Ola Habanjar, Mona Diab-Assaf, Florence Caldefie-Chezet, Laetitia Delort
Summary: The 3D culture system has become essential in cancer research and tissue engineering for its ability to mimic tumor microenvironment and provide better biocompatibility. Different types of natural supports, synthetic strategies, and methods for generating normal and tumor spheroids are key factors in constructing ideal 3D models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Da-Hyun Kim, Jungho Ahn, Hyun Kyoung Kang, Min-Soo Kim, Nam-Gyo Kim, Myung Geun Kook, Soon Won Choi, Noo Li Jeon, Heung-Myong Woo, Kyung-Sun Kang
Summary: Liver tissue engineering with vascular reconstruction using decellularized liver scaffolds showed enhanced liver-specific functions and perfusable vessel networks, providing a promising strategy for liver failure treatment.
Article
Engineering, Biomedical
Zhuo Li, Haixing Wang, Kunyu Zhang, Boguang Yang, Xian Xie, Zhengmeng Yang, Lingchi Kong, Peng Shi, Yuan Zhang, Yi-Ping Ho, Zhi-Yong Zhang, Gang Li, Liming Bian
Summary: The research assembled a biomimetic hyaluronic acid nanocomposite hydrogel with bisphosphonates (BPs), and demonstrated its expedited release of BPs in an acidic environment. The in vitro studies showed that the hydrogel inhibits mature osteoclastic differentiation, while promoting the initial differentiation of primary macrophages to preosteoclasts. In vivo evaluation confirmed the hydrogel's ability to enhance in situ bone regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Monami Yamane, Jieun Seo, Yinghui Zhou, Tomoki Asaba, Shan Tu, Ayaka Nanmo, Tatsuto Kageyama, Junji Fukuda
Summary: Transcriptome comparisons of dermal papilla cells (DPCs) in hair beads (HB) culture and typical 2D culture revealed the upregulation of the phosphoinositide 3-kinase and Akt (PI3K/Akt) signaling pathway in HB culture. Inhibition of this pathway decreased the hair-inducing capability of DPCs in HBs, while activation of the pathway improved trichogenous gene expression of DPCs in 2D culture.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Yusuke Kimura, Hironori Saito, Tatsuya Osaki, Yasuhiro Ikegami, Taisei Wakigawa, Yoshiho Ikeuchi, Shintaro Iwasaki
Summary: Mitochondria have their own genome that encodes components of oxidative phosphorylation complexes, and mitochondrial ribosomes translate the mRNAs expressed from the mitochondrial genome. Cellular heterogeneity in mitochondrial translation can be expected due to the differential OXPHOS activity observed in different cell types and growth conditions. In this study, researchers developed a technique called mito-FUNCAT-FACS that allows the labeling and sorting of newly synthesized mitochondrial proteins using a click chemistry-compatible methionine analog. This approach provides a useful tool for examining mitochondrial protein synthesis in individual cells.
Article
Engineering, Biomedical
Ayaka Nanmo, Lei Yan, Tomoki Asaba, Licheng Wan, Tatsuto Kageyama, Junji Fukuda
Summary: This study proposes a scalable and automated approach for the preparation of highly hair-inductive grafts using a bioprinter. The method utilizes collagen droplets to facilitate hair follicle regeneration through cell interactions. This approach holds great potential for clinical applications.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Hyeonyu Kim, Tatsuya Osaki, Roger D. Kamm, H. Harry Asada
Summary: This study develops an engineered muscle tissue with a coaxial three-layered tubular structure that resembles the architecture of native skeletal muscles. By remodeling the extracellular matrix, the engineered skeletal muscle tissues produced stronger contractile force and improved angiogenesis.
Article
Chemistry, Multidisciplinary
Yuka Sawada, Hanae Tsukumo, Junji Fukuda, Kazutoshi Iijima, Hiroshi Itagaki
Summary: To improve the accuracy of predicting chemical skin sensitization using cell-based alternative methods, researchers developed a skin sensitization test system using THP-1 cells as a substitute for dendritic cells and keratinized normal human epidermal keratinocytes (NHEK). The expression of CD54 and CD86 on THP-1 cells was measured after exposure to chemicals via keratinized NHEK, and it was found that the composition of the medium has a significant effect on the evaluation index in the co-culture system.
APPLIED SCIENCES-BASEL
(2022)
Article
Oncology
Shunsuke Kitajima, Tetsuo Tani, Benjamin F. Springer, Marco Campisi, Tatsuya Osaki, Koji Haratani, Minyue Chen, Erik H. Knelson, Navin R. Mahadevan, Jessica Ritter, Ryohei Yoshida, Jens Kohler, Atsuko Ogino, Ryu-Suke Nozawa, Shriram K. Sundararaman, Tran C. Thai, Mizuki Homme, Brandon Piel, Sophie Kivlehan, Bonje N. Obua, Connor Purcell, Mamiko Yajima, Thanh U. Barbie, Patrick H. Lizotte, Pasi A. Janne, Cloud P. Paweletz, Prafulla C. Gokhale, David A. Barbie
Summary: KRAS-LKB1 mutant lung cancers silence the STING pathway and minimize intracellular accumulation of 2'3'-cGAMP to avoid T cell infiltration and decrease response to PD-1 blockade. However, transient MPS1 inhibition can re-activate the STING pathway in KL cells and restore T cell infiltration, enhancing the efficacy of anti-PD-1 treatment.
Article
Biotechnology & Applied Microbiology
Tatsuto Kageyama, Riki Anakama, Hideru Togashi, Junji Fukuda
Summary: This study investigated the effects of cell sorting manipulation on hair regeneration in vitro using Y27632. The supplementation of Y27632 modulated the spatial distribution of cells and promoted the development of hair follicle organoids. The study also revealed the involvement of specific signaling pathways in the process.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Engineering, Biomedical
Hyeonyu Kim, Tatsuya Osaki, Roger D. Kamm, H. Harry Asada
Summary: Creating a vasculature in engineered human skeletal muscle tissues has various advantages, including increasing tissue thickness, improving cell survival, providing models for drug testing, and enhancing muscle differentiation. The researchers demonstrated the creation of contractile skeletal muscle tissues with a central vascular channel and microvascular networks. By recreating the in vivo extracellular fluid compartments, they were able to construct large vascularized skeletal muscle tissues for drug testing platforms. Coculturing with incompatible media from an early stage of muscle differentiation led to improved muscle differentiation and contractile force.
Article
Cell Biology
Siu Yu A. Chow, Kazuki Nakayama, Tatsuya Osaki, Maki Sugiyama, Maiko Yamada, Hirotaka Takeuchi, Yoshiho Ikeuchi
Summary: It is discovered that there is more contact between melanocytes and nociceptive neurons in human skin color patch tissue. Co-culture with iPSC-derived sensory neurons promotes morphogenesis and pigmentation of melanocytes. RGMB is identified as a melanocyte-stimulating factor released from sensory neurons. Transcriptome analysis suggests that RGMB can control the melanosome transport machinery of melanocytes.
Article
Multidisciplinary Sciences
Tatsuto Kageyama, Akihiro Shimizu, Riki Anakama, Rikuma Nakajima, Kohei Suzuki, Yusuke Okubo, Junji Fukuda
Summary: This study demonstrates the successful induction of hair follicle formation in vitro by controlling the interactions between epithelial and mesenchymal cells. The in vitro hair follicle model has potential applications in understanding hair follicle induction, evaluating drug effects on hair growth and inhibition, and modeling gray hairs.
Article
Materials Science, Biomaterials
Sugi Hirano, Tatsuto Kageyama, Maki Yamanouchi, Lei Yan, Kohei Suzuki, Katsumi Ebisawa, Keiichiro Kasai, Junji Fukuda
Summary: Hair regeneration using HFSCs and dermal papilla cells has potential for alopecia treatment. This study developed a novel approach for HFSC expansion through cell aggregates formation and Matrigel encapsulation. The HFSCs cultured using a microarray device showed a 20-fold increase in quantity and higher expression of trichogenic stem cell markers compared to conventional culture methods. HFSCs cultured using this method combined with embryonic mesenchymal cells demonstrated vigorous hair regeneration in nude mice. Moreover, the expansion of human HFSCs from androgenetic alopecia patients showed significant improvement using this approach. Therefore, this study proposes a useful method for HFSC expansion in hair regenerative medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Mio Aoki, Ryoto Yokota, Shoji Maruo, Tatsuto Kageyama, Junji Fukuda
Summary: This study investigated the efficacy of slow vitrification methods for freezing hair follicle germs (HFGs) and proposed a microfinger device to scale up the cryopreservation process. The results showed that DMSO vitrification yielded efficient hair regeneration, comparable to non-cryoprotected HFGs, and the microfinger device achieved over 50% hair regeneration rate for cryopreserved HFGs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Do-Won Jeong, Jong-Wan Park, Kyeong Seog Kim, Jiyoung Kim, June Huh, Jieun Seo, Ye Lee Kim, Joo-Youn Cho, Kwang-Woong Lee, Junji Fukuda, Yang-Sook Chun
Summary: This study demonstrates that palmitic acid (PA) acts as a molecular checkpoint of lipid reprogramming in liver cancer cells. PA mediates the palmitoylation of PHF2, leading to ubiquitin-dependent degradation of PHF2 and reducing lipogenesis. Additionally, PHF2 functions as an E3 ubiquitin ligase and destabilizes the master transcription factor of lipogenesis, SREBP1c. The induction of PA triggers the PHF2/SREBP1c axis, which increases lipogenesis in hepatocellular carcinoma (HCC). Therefore, monitoring dietary PA levels may be important for HCC patients.
NATURE COMMUNICATIONS
(2023)