Article
Fisheries
Baoli Zhang, Changyue Yu, Yingkai Xu, Ziwei Huang, Yuqiao Cai, Yingdong Li
Summary: Photoperiod plays a vital role in the growth, development, and metabolism of crustaceans. The study revealed that the photoperiod affects the gene expression in the hepatopancreas of Chinese mitten crab, leading to changes in immune mechanisms. High-throughput Illumina sequencing was used to explore the genes associated with immune function, and the results demonstrated the impact of photoperiod on gene expression patterns.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Peng Huang, Liping Cao, Jinliang Du, Jiancao Gao, Yuning Zhang, Yi Sun, Quanjie Li, Zhijuan Nie, Gangchun Xu
Summary: There is growing evidence that long-term exposure to the widely used herbicide prometryn can induce toxicity in bony fish and shrimp. This study investigated the effects of prometryn on the hepatopancreas and intestine of the crab Eriocheir sinensis through a 20-day exposure experiment. The findings suggest that prometryn can reduce antioxidant enzyme activities, increase lipid peroxidation levels, and cause oxidative stress.
Article
Biotechnology & Applied Microbiology
Hongli Liu, Yang Feng, Ma Yang, Ya Huang, Minghao Li, Yi Geng, Ping Ouyang, Defang Chen, Shiyong Yang, Lizi Yin, Liangyu Li, Xiaoli Huang
Summary: Research reveals that starvation leads to a decrease in the hepatopancreas index of Chinese mitten crab, affecting tissue structure, metabolism, and angiogenesis. The AKT signaling pathway plays a crucial role in this process, regulating gene expression related to angiogenesis and apoptosis, ultimately causing hepatopancreas atrophy.
Article
Food Science & Technology
Licheng Fan, Caining Xian, Shijie Tang, Wei Ding, Chang-Hua Xu, Xi-Chang Wang
Summary: The study found that the level of lipid oxidation in the hepatopancreas of Eriocheir sinensis varied at different temperatures, with the lowest oxidation observed at -80 degrees Celsius. A back propagation neural network with 10 neurons was effectively used to predict lipid stability during frozen storage with high accuracy. Evaluation of changes in nutritional quality using atherogenesis and thrombosis indexes proved beneficial in understanding the impact of frozen storage conditions on the samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Yuning Zhang, Jiancao Gao, Liping Cao, Jinliang Du, Gangchun Xu, Pao Xu
Summary: With climate warming, blue-green algae blooms have increased in frequency and severity, releasing microcystin toxins that pose potential threats to health. This study found that MC-LR activates autophagy in the hepatopancreas of Chinese mitten crabs, and identified miR-282-5p and PIK3R1 as important regulators of autophagy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Food Science & Technology
Qiu-Ju Wang, Bao-Yuan Zhang, Xiao-Dong Jiang, Xiao-Wen Long, Wang-Ling Zhu, Yong-Ping Xu, Min Wu, Dong-Ming Zhang
Summary: The study found that adult female Chinese mitten crabs with white hepatopancreas syndrome had significantly higher moisture content in their hepatopancreas and ovaries compared to the control group, while hepatopancreas crude fat and ovary crude protein levels were significantly lower. The yellow-white hepatopancreas group had significantly higher total carbohydrate levels and significantly lower crude protein content in ovaries. Additionally, levels of certain amino acids and fatty acids were significantly altered in diseased crabs, with the white hepatopancreas group showing poor nutritional quality.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Limnology
Yi Zhang, Mengqi Ni, Jinbin Zheng, Zhaoxia Cui
Summary: This study revealed different mechanisms adopted by male and female Chinese mitten crabs in response to air exposure stress, with females showing a preference for increasing heat shock protein expression and males tending to resist stress by increasing antioxidant enzyme expression. Overall, it provides novel insights into the molecular mechanisms underlying the air exposure stress response of E. sinensis.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Food Science & Technology
Long Zhang, Ning-Ping Tao, Xugan Wu, Xichang Wang
Summary: Chinese mitten crab, as an important seafood in China, is affected by the color and bitterness of its hepatopancreas, which can significantly impact consumer acceptance. This study investigated the metabolic relationship between key metabolites and the color and bitterness in the hepatopancreas of the crab. The results suggest that bile acids and bitter free amino acids may contribute to the bitterness of the hepatopancreas.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Genetics & Heredity
Yu Xu, Zhen Miao, Xuguang Li, Hai Lin, Yu Cheng, Jianlin Pan, Zhiqiang Xu
Summary: Hypoxia is a common yet overlooked issue in Chinese mitten crab rearing. This study found that the hepatopancreas transcriptome of crabs is severely affected under hypoxia, with significant changes in gene expression related to cellular adaptive responses to hypoxia. The results suggest that even after 12 hours of reoxygenation, the adverse effects of hypoxia on growth, development, and disease susceptibility in the crabs persist.
Article
Toxicology
Cheng Chi, Xia-Wei Yu, Cai-Yan Zhang, Jia-Dai Liu, Ming-Wen Ye, Ding-Dong Zhang, Wen-Bin Liu
Summary: Chinese mitten crab is economically important in aquaculture, but hepatopancreatic necrosis syndrome caused by cyanobacteria bloom outbreaks and MC-LR toxicity can affect its quality and breeding industry.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Environmental Sciences
Yuhang Hong, Yi Huang, Yanzhen Dong, Dayong Xu, Qiang Huang, Zhiqiu Huang
Summary: This study investigated the cytotoxic effects of abamectin on hepatopancreas cells of Chinese mitten crab. The results showed that abamectin inhibited cell viability and caused oxidative stress, DNA damage, and apoptosis. It also induced changes in antioxidant response and immune status.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Immunology
Libo Hou, Mengdi Wang, Lei Zhu, Mingxiao Ning, Jingxiu Bi, Jie Du, Xianghui Kong, Wei Gu, Qingguo Meng
Summary: As a new generation of high-throughput sequencing technology, PacBio Iso-Seq technology provides a better alternative sequencing method for obtaining full-length unigenes. In this study, a comprehensive analysis of the Eriocheir sinensis transcriptome was conducted using Iso-Seq and Illumina RNA-seq analysis. Key genes related to H. eriocheir infection in the intestines of E. sinensis were identified, and important biological pathways such as energy metabolism and innate immune response were revealed. These findings enhance our understanding of the E. sinensis transcriptome and its specific association with H. eriocheir infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mingyu Yin, Xueyan Fu, Xichang Wang
Summary: This study investigated the changes in lipid composition of female Chinese mitten crabs using UHPLC-Q E Orbitrap mass spectrometer in a simulated thermal oxidation system. The key lipids identified were phosphatidylethanolamine (PE) and free fatty acid (FFA), with key fatty acids including C18:1, C18:3, C20:3, C20:4, C20:5, and C22:6. The degradation rate of C24:0 in FFA was found to be higher than the synthesis rate. Analysis of the molecular species provided insights into the dynamic change mechanism of lipids in the hepatopancreas, with potential implications for product processing and sensory preferences of the public.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lin Zhou, Lanlu Chu, Jinliang Du, Zhijuan Nie, Liping Cao, Jiancao Gao, Gangchun Xu
Summary: Chinese mitten crab (E. sinensis) is an important farmed crustacean in China, but farming process may lead to the occurrence of harmful factor, lipopolysaccharide (LPS). This study investigated the correlation between LPS concentrations and hepatopancreas damage in E. sinensis. Results showed that LPS significantly affected immune-related genes and antioxidant enzyme activity, resulting in hepatopancreas damage. These findings provide valuable insights into the mechanism of hepatopancreas injury induced by LPS infection in E. sinensis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Environmental Sciences
Peng Huang, Jinliang Du, Liping Cao, Jiancao Gao, Quanjie Li, Yi Sun, Nailin Shao, Yuning Zhang, Gangchun Xu
Summary: In this study, the toxic effects of the triazine pesticide prometryn on Eriocheir sinensis were evaluated. The results showed that prometryn can cause damage to the hepatopancreas of E. sinensis through oxidative stress, induction of cell apoptosis, disruption of lipid metabolism, and immune damage.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)