Article
Cell Biology
Marino DiFranco, Stephen C. Cannon
Summary: Hypokalemic periodic paralysis (HypoPP) is a skeletal muscle channelopathy caused by mutations in voltage sensor domains of calcium or sodium channels. Recurrent attacks of weakness are the primary clinical manifestation, resulting from impaired excitability of depolarized fibers containing leaky mutant channels. Reduced Ca2+-release in Ca(V)1.1 mutant channels may contribute to the late-onset permanent muscle weakness in Ca(V)1.1-associated HypoPP. The episodes of transient weakness in both Ca(V)1.1 and Na(V)1.4-associated HypoPP are primarily caused by leaky channels and are not a consequence of reduced Ca2+-release.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Food Science & Technology
Tertius A. Kohn, Megan J. Anley, S'thandiwe N. Magwaza, Luqmaan Adamson, Louw C. Hoffman, Tertius S. Brand
Summary: This study analyzed muscle fiber type, fiber cross-sectional area, enzyme activities, and glycogen content in four muscles of 24 ostriches. The results showed similar proportions of type I and II fibers in all muscles, with the smallest fibers in the iliotibialis cranialis muscle. Citrate synthase activity was highest in the iliotibialis cranialis muscle and similar in the other muscles. 3-hydroxyacetyl Co A dehydrogenase activities were low in all muscles, indicating poor beta-oxidation. The iliotibialis cranialis muscle also had the lowest phosphofructokinase activity. Glycogen content varied within each muscle. These findings suggest that the ostrich muscles have low fat oxidation capacity and glycogen content, which could affect meat quality attributes.
Article
Agriculture, Dairy & Animal Science
Nicolas Busse, Madison L. Gonzalez, Ashley L. Wagner, Sally E. Johnson
Summary: This study found that supplementation with calcium butyrate in adult Thoroughbred geldings increased the percentage of type IIA fibers and reduced the numbers of type IIX fibers in the gluteus medius muscle. The increase in type IIA fibers suggests that calcium butyrate may affect myosin ATPase expression independent of altered metabolism. Further research is needed to understand the mechanism of action and validate the improved exercise performance in response to calcium butyrate supplementation in equine skeletal muscle.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Pablo E. Morales, Matias Monsalves-Alvarez, Satya Murthy Tadinada, Matthew P. Harris, Andrea Ramirez-Sagredo, Jafet Ortiz-Quintero, Mayarling Francisca Troncoso, Nicole De Gregorio, Ximena Calle, Renata O. Pereira, Vitor A. Lira, Alejandra Espinosa, E. Dale Abel, Sergio Lavandero
Summary: The study found that high-fat diet induced differential regulation of autophagy and mitochondrial activity in different skeletal muscles of mice. After high-fat diet feeding, oxidative soleus muscle showed increased OXPHOS subunits and mitochondrial DNA content, while gastrocnemius muscle exhibited an increase in mitochondrial mass only after 40 weeks. Defects in autophagy may alter mitochondrial respiratory activity in response to obesogenic diet.
Article
Biochemistry & Molecular Biology
Christopher G. Vann, Robert W. Morton, Christopher B. Mobley, Ivan J. Vechetti, Brian K. Ferguson, Cody T. Haun, Shelby C. Osburn, Casey L. Sexton, Carlton D. Fox, Matthew A. Romero, Paul A. Roberson, Sara Y. Oikawa, Chris McGlory, Kaelin C. Young, John J. McCarthy, Stuart M. Phillips, Michael D. Roberts
Summary: This study examined the association between genotype and resistance training-induced changes in lean soft tissue mass and muscle fiber cross-sectional area, identifying potential links between GLI3 gene variant and hypertrophic responses. Future studies are needed to confirm this association.
Article
Multidisciplinary Sciences
Toshiko Yamazawa, Takuya Kobayashi, Nagomi Kurebayashi, Masato Konishi, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Shuichi Mori, Hiroto Iinuma, Noriaki Manaka, Hiroyuki Kagechika, Arkady Uryash, Jose Adams, Jose R. Lopez, Xiaochen Liu, Christine Diggle, Paul D. Allen, Sho Kakizawa, Keigo Ikeda, Bangzhong Lin, Yui Ikemi, Kazuto Nunomura, Shinsaku Nakagawa, Takashi Sakurai, Takashi Murayama
Summary: The study shows that an oxolinic acid-derivative RyR1 inhibitor can effectively prevent and treat various malignant hyperthermia (MH) and heat stroke mouse models.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Kotaro Oyama, Vadim Zeeb, Toshiko Yamazawa, Nagomi Kurebayashi, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Hideto Oyamada, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Yoshie Harada, Norio Fukuda, Shin'ichi Ishiwata, Madoka Suzuki
Summary: This study investigates the relationship between mutant Ryanodine receptor type 1 (RyR1) and malignant hyperthermia (MH) using an optically controlled local heat-pulse method. The results show that RyR1 mutants are more heat sensitive and induce intracellular calcium bursts through heat-induced calcium release (HICR). HICR is also observed in skeletal muscles. This study highlights the complexity of MH and the positive feedback of HICR in thermogenesis in MH patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Physiology
Mizuki Sudo, Yutaka Kano, Soichi Ando
Summary: This study investigated the effects of environmental enrichment on muscle adaptation in rats and found that environmental enrichment can induce hypertrophy of slow-twitch muscle fibers through increased daily voluntary physical activity.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biology
Xueyong Wang, Murad Nawaz, Chris DuPont, Jessica H. Myers, Steve Ra Burke, Roger A. Bannister, Brent D. Foy, Andrew A. Voss, Mark M. Rich
Summary: Excitation-contraction coupling is the process of converting electrical excitation of muscle into force generation. Depolarization of skeletal muscle can lead to failure of ECC, specifically the failure to generate calcium transients. Different AP properties, such as AP peak and AP integral, play important roles in different steps of ECC, including AP conduction and calcium release.
Article
Agriculture, Dairy & Animal Science
Luke K. Fuerniss, Bradley J. Johnson
Summary: This study validated a semi-automated workflow that can efficiently and objectively identify the type and measure the size of muscle fibers compared to a manual technique. The semi-automated workflow is six times faster than the manual workflow and yields similar results. This study demonstrates the efficiency and objectivity of the semi-automated workflow in imaging, classifying, and measuring muscle fibers.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Sport Sciences
Christopher G. Vann, Cody T. Haun, Shelby C. Osburn, Matthew A. Romero, Paul A. Roberson, Petey W. Mumford, C. Brooks Mobley, Hudson M. Holmes, Carlton D. Fox, Kaelin C. Young, Michael D. Roberts
Summary: This study found that one week of active recovery after high-intensity training block instigates marginal molecular differences in skeletal muscle compared to passive recovery. However, from a practical standpoint, both paradigms elicited largely similar responses.
JOURNAL OF STRENGTH AND CONDITIONING RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Martina Baraldo, Leonardo Nogara, Georgia Ana Dumitras, Achille Homere Tchampda Dondjang, Alessia Geremia, Marco Scalabrin, Clara Turk, Frederik Telkamp, Lorena Zentilin, Mauro Giacca, Marcus Kruger, Bert Blaauw
Summary: Loss of Raptor diminishes muscle hypertrophy and force increase after Akt activation, indicating mTORC1 as the key mediator of Akt-dependent muscle growth regulating the mitochondrial proteome critical for enhancing muscle force.
Article
Nutrition & Dietetics
J. Max Michel, Kristy K. Lievense, Sam C. Norton, Juliana V. Costa, Kathryn H. Alphin, Lydia A. Bailey, Gary D. Miller
Summary: This study examined the effects of resistance training (RT) and protein intake on muscle in older adults. The results showed that RT improved muscle quality and function, and the type of protein intake did not have a significant impact on the outcomes.
Article
Biotechnology & Applied Microbiology
M. A. Greene, A. N. S. Udoka, R. R. Powell, R. E. Noorai, T. Bruce, S. K. Duckett
Summary: This study investigated the impact of in utero mycotoxin exposure on skeletal muscle fiber hypertrophy and the miRNA profile in lambs. The results showed that exposure to mycotoxins did not alter fiber type but had long-term effects on postnatal muscle hypertrophy and cross-sectional area. Additionally, developmental age influenced the miRNA transcriptome and mRNA expression of genes related to muscle growth.
Article
Genetics & Heredity
Bruna Petry, Gabriel Costa Monteiro Moreira, Aline Goncalves Lio Copola, Marcela Maria de Souza, Fernanda Cristina da Veiga, Erika Cristina Jorge, Jane de Oliveira Peixoto, Monica Correa Ledur, James E. Koltes, Luiz Lehmann Coutinho
Summary: This study investigated the role of SAP30 gene in muscle development by downregulating it in C2C12 muscle cell culture and analyzing its impact on morphometric traits and gene expression. The results showed that modulation of SAP30 expression increased myotube area, indicating its involvement in muscle hypertrophy. RNA-seq analysis further supported the role of SAP30 gene in regulating muscle development.
FRONTIERS IN GENETICS
(2021)
Article
Biotechnology & Applied Microbiology
Jean-Baptiste Dupont, Jianjun Guo, Edith Renaud-Gabardos, Karine Poulard, Virginie Latournerie, Michael W. Lawlor, Robert W. Grange, John T. Gray, Ana Buj-Bello, Martin K. Childers, David L. Mack
Review
Physiology
Deborah J. Good, Haiyan Zhang, Robert W. Grange, Thomas Braun
EXERCISE AND SPORT SCIENCES REVIEWS
(2020)
Article
Cell Biology
Claire Yuan, Ashwin Arora, Anthony M. Garofalo, Robert W. Grange
Summary: This study explores the potential signaling between dystrophic skeletal muscle and tendon in Duchenne muscular dystrophy, focusing on the cross-talk at the myotendinous junction. The absence of dystrophin and the associated dystrophin-glycoprotein complex is a key feature of Duchenne muscular dystrophy, with other potential signal pathways contributing to the cross-talk between muscle and tendon.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Physiology
Katherine E. Bukovec, Xiao Hu, Matthew Borkowski, Duane Jeffery, Silvia S. Blemker, Robert W. Grange
JOURNAL OF APPLIED PHYSIOLOGY
(2020)
Article
Physiology
Sabah N. Rezvani, Anne E. C. Nichols, Robert W. Grange, Linda A. Dahlgren, P. Gunnar Brolinson, Vincent M. Wang
Summary: Achilles tendinopathy is a challenging condition with limited effective therapies. This study introduced a novel mouse model of hindlimb muscle loading for targeted therapeutic exercise, showing promising results in improving biomechanical outcomes in a murine tendinopathy model. This model opens up possibilities for further research on how muscle loading can enhance healing of Achilles tendon injuries.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Cell Biology
Goutam Chandra, Sen Chandra Sreetama, Davi A. G. Mazala, Karine Charton, Jack H. VanderMeulen, Isabelle Richard, Jyoti K. Jaiswal
Summary: This passage discusses the important role of the endoplasmic reticulum in maintaining physiological calcium balance and cell membrane repair, highlighting the crucial functions of SERCA and ANO5 in regulating intracellular calcium levels.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Shelby E. Hamm, Daniel D. Fathalikhani, Katherine E. Bukovec, Adele K. Addington, Haiyan Zhang, Justin B. Perry, Ryan P. McMillan, Michael W. Lawlor, Mariah J. Prom, Mark A. Vanden Avond, Suresh N. Kumar, Kirsten E. Coleman, J. B. Dupont, David L. Mack, David A. Brown, Carl A. Morris, J. Patrick Gonzalez, Robert W. Grange
Summary: The study showed that combining voluntary wheel running with microdystrophin gene therapy in young mdx mice improved whole-body performance, affected muscle function to varying degrees, mitigated energy deficits, but also revealed some detrimental effects of exercise.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Cell Biology
James S. Novak, Davi A. G. Mazala, Marie Nearing, Ravi Hindupur, Prech Uapinyoying, Nayab F. Habib, Tessa Dickson, Olga B. Ioffe, Brent T. Harris, Marie N. Fidelia-Lambert, Christopher T. Rossi, D. Ashely Hill, Kathryn R. Wagner, Eric P. Hoffman, Terence A. Partridge
Summary: The age-related loss of muscle mass and strength is likely not caused by intrinsic loss of myogenicity in satellite cells, but rather by progressive and detrimental changes in the muscle microenvironment that affect the myogenic function of these cells.
Editorial Material
Biochemistry & Molecular Biology
Marshall W. Hogarth, Prech Uapinyoying, Davi A. G. Mazala, Jyoti K. Jaiswal
Summary: In addition to myofibers, there are many mononucleated cells in skeletal muscle, including fibro-adipogenic progenitors (FAPs), which support muscle development and regeneration in adults. Recent evidence suggests that defects in FAP function contribute to chronic muscle diseases, making targeting FAPs a potential avenue for treating these diseases.
TRENDS IN MOLECULAR MEDICINE
(2022)
Article
Nutrition & Dietetics
Brittney Knott, Matthew A. Kocher, Henry A. Paz, Shelby E. Hamm, William Fink, Jordan Mason, Robert W. Grange, Umesh D. Wankhade, Deborah J. Good
Summary: This study demonstrated that Snord116(m+/p-) mice and mice with a deletion of both Snord116 alleles showed weight and fat loss on a high-fat/CLA diet, indicating that the genotype did not interfere with CLA actions. There were no changes in food intake or metabolic rate, and only moderate differences in exercise performance. RNA-seq and microbiome analyses identified hypothalamic mRNAs and differentially populated gut bacteria, supporting future mechanistic analyses.
Correction
Medicine, Research & Experimental
Shelby E. Hamm, Daniel D. Fathalikhani, Katherine E. Bukovec, Adele K. Addington, Haiyan Zhang, Justin B. Perry, Ryan P. McMillan, Michael W. Lawlor, Mariah J. Prom, Mark A. Vanden Avond, Suresh N. Kumar, Kirsten E. Coleman, J. B. Dupont, David L. Mack, David A. Brown, Carl A. Morris, J. Patrick Gonzalez, Robert W. Grange
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Cell Biology
Michael Haug, Barbara Reischl, Stefanie Nuebler, Leonit Kiriaev, Davi A. G. Mazala, Peter J. Houweling, Kathryn N. North, Oliver Friedrich, Stewart Head
Summary: The polymorphism in the ACTN3 gene affects the organization and mechanical strength of fast-twitch muscle fibers, leading to structural weakness and a rapid decline in muscle function with increasing age.
Article
Cell Biology
Davi A. G. Mazala, Ravi Hindupur, Young Jae Moon, Fatima Shaikh, Iteoluwakishi H. Gamu, Dhruv Alladi, Georgiana Panci, Michele Weiss-Gayet, Benedicte Chazaud, Terence A. Partridge, James S. Novak, Jyoti K. Jaiswal
Summary: Lack of dystrophin expression is the genetic basis for Duchenne muscular dystrophy (DMD), and disease severity varies due to genetic modifiers. The D2-mdx model exhibits severe muscle degeneration and poor regeneration in juvenile stage, resulting from an enhanced inflammatory response and excessive accumulation of fibroadipogenic progenitors (FAPs). However, in adult D2-mdx muscle, the extent of damage and degeneration decreases, associated with restoration of inflammatory and FAP responses, leading to improved regenerative myogenesis. Therefore, targeting aberrant stromal cell responses may provide therapeutic benefits for DMD treatment.
CELL DEATH DISCOVERY
(2023)
Article
Cell Biology
Goutam Chandra, Davi A. G. Mazala, Jyoti K. Jaiswal
Summary: Cells utilize ER-driven Cl- uptake to sequester injury-induced cytosolic Ca2+ overload, which is crucial for maintaining cellular function and repair response. Lack of ER-resident Anoctamin 5 (ANO5) contributes to difficulties in coping with Ca2+ overload, leading to muscular dystrophy.
Article
Medicine, Research & Experimental
Davi A. G. Mazala, James S. Novak, Marshall W. Hogarth, Marie Nearing, Prabhat Adusumalli, Christopher B. Tully, Nayab F. Habib, Heather Gordish-Dressman, Yi-Wen Chen, Jyoti K. Jaiswal, Terence A. Partridge