Article
Biochemistry & Molecular Biology
Claudia Lennicke, Helena M. Cocheme
Summary: The study found that oxidants, especially H2O2, can mimic the effects of insulin. Increasing intracellular H2O2 levels affects signaling and amplifies insulin-mediated cellular responses. Specific molecular targets of insulin-stimulated H2O2 include phosphatases and kinases, whose activity is altered through redox modifications.
Review
Biochemistry & Molecular Biology
Nicholas J. Day, Matthew J. Gaffrey, Wei-Jun Qian
Summary: The review highlights how mass spectrometry-based redox proteomics has enabled accurate quantification of reversible oxidative modifications on specific cysteine residues of proteins, emphasizing the significance and application of such measurements in the field of redox biology.
Review
Biochemistry & Molecular Biology
Beatriz Castejon-Vega, Mario D. D. Cordero, Alberto Sanz
Summary: In the past, mtROS were seen as byproducts of cellular metabolism and drivers of ageing and age-related diseases. Now, we know that mtROS serve as important cellular messengers, playing a role in maintaining cellular homeostasis and influencing cellular differentiation, proliferation, and survival. However, dysregulated mtROS signaling can contribute to degenerative diseases. This review focuses on the signaling pathways involving mtROS and their role in ageing.
Review
Biochemical Research Methods
Xiaolu Li, Austin Gluth, Tong Zhang, Wei-Jun Qian
Summary: Redox post-translational modifications greatly impact protein structure and function, allowing for the regulation of biological processes. Redox proteomics approaches use mass spectrometry to study these modifications and have contributed to our understanding of redox signaling and regulation. Various strategies, including blocking free thiols and using selective reduction and enrichment techniques, are employed to analyze redox PTMs. This review discusses the principles and recent advances in redox proteomics and highlights its applications in generating novel biological insights.
Review
Biochemistry & Molecular Biology
Lara Vogelsang, Karl -Josef Dietz
Summary: The temporal and spatial patterns of reactive oxygen species (ROS) in cells and tissues determine the plant's response to various stresses. Recent progress in dynamic cell imaging probes and sensitive proteomics has provided important information on the cellular redox state. Thiol peroxidases play a central role in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Filippo Scialo, Alberto Sanz
Summary: Mitochondria are essential for producing energy in cells, but they also generate Reactive Oxygen Species (ROS) which play a critical role in determining cell fate and maintaining cellular homeostasis. Accumulation of defective mitochondria during aging leads to high levels of ROS production, causing oxidative stress and disrupting redox signaling.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ting Luo, Julia Malo Pueyo, Khadija Wahni, Charlotte Yvanoff, Tamas Lazar, Sebastien Pyr dit Ruys, Didier Vertommen, Daria Ezerin, Joris Messens
Summary: Hydrogen peroxide acts as a signaling molecule by oxidizing cysteine thiols in proteins, and recent studies have shown the role of cytosolic peroxiredoxins in transmitting this oxidation to target proteins. Two thiol-disulphide independent in-cell trapping methods, BioID and crosslinker co-immunoprecipitation, coupled with mass spectrometry were used to identify interaction partners of Prdx2, with BioID proving to be a more reliable method. The study identified 13 interactors under elevated H2O2 conditions, including CSN5, and demonstrated the potential of BioID in identifying Prdxs interactors.
Review
Biochemistry & Molecular Biology
Lisa R. Knoke, Lars I. Leichert
Summary: Due to its nucleophilicity, the thiol group of cysteine plays an important role in protein chemistry. The nucleophilicity of the thiol group makes cysteine susceptible to oxidative modifications, which can alter protein function and are reversible in vivo. Therefore, studying oxidative posttranslational modifications of cysteine is crucial for understanding redox biology.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Marcos Martinez-Banaclocha
Summary: Significant progress has been made in understanding the pathophysiology of age-associated neurodegenerative diseases in the last twenty years. However, there is still a lack of clinically significant therapeutic advancement in the prevention and treatment of these diseases. It is important to focus on early diagnosis and prompt intervention to slow down disease progression and improve patients' quality of life.
Article
Biochemistry & Molecular Biology
Che S. Pillay, Nolyn John, Christopher J. Barry, Lulama M. D. C. Mthethwa, Johann M. Rohwer
Summary: Infectious diseases pose a significant health burden, especially with the emergence of drug-resistant strains in developing countries. Understanding the factors that contribute to pathogen persistence, such as Mycobacterium tuberculosis and Plasmodium falciparum, is crucial. Pathogen antioxidant defenses, such as peroxiredoxin and thioredoxin systems, play critical roles in their ability to tolerate oxidative stress.
Article
Biochemistry & Molecular Biology
Ahmet Tuncay, Anna Noble, Matthew Guille, James N. Cobley
Summary: RedoxiFluor is a novel antibody technology that enables accurate quantification of target-specific protein thiol redox state in percentages and moles on a microplate. It can operate in global mode and array mode, making it highly versatile. Extensive validation in diverse biological contexts demonstrates the reliability and utility of this technology.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Daniel Maynard, Andrea Viehhauser, Madita Knieper, Anna Dreyer, Ghamdan Manea, Wilena Telman, Falk Butter, Kamel Chibani, Renate Scheibe, Karl-Josef Dietz
Summary: Alpha, beta-unsaturated carbonyls can react with thiols of metabolites like cysteine and glutathione in plant physiological processes. Compounds such as 12-oxophytodienoic acid and abscisic acid conjugate with cysteine, with varying reactivities towards glutathione. Furthermore, these compounds interact with proteins like members of the thioredoxin-fold family, affecting their activity in cellular regulation.
Article
Biochemistry & Molecular Biology
Arjan de Groot, Laurence Blanchard, Nicolas Rouhier, Pascal Rey
Summary: Deinococcus species have remarkable tolerance to oxidative damage in extreme environmental conditions. Thiol reductases (TRs) play important roles in metabolism regulation and stress responses. The study reveals that Deinococcus possesses multiple enzyme systems that protect proteins and maintain redox status. Recent research further confirms the importance of TRs in Deinococcus' tolerance to oxidative treatments.
Article
Biochemistry & Molecular Biology
Lotem Sarid, Eva Zanditenas, Jun Ye, Meirav Trebicz-Geffen, Serge Ankri
Summary: This study investigates the amebicidal activity of L. acidophilus, a probiotic, against E. histolytica. The results show that L. acidophilus reduces the viability of E. histolytica trophozoites by 50% through the production of hydrogen peroxide and the oxidation of essential amebic enzymes. Additionally, incubation with L. acidophilus decreases the binding of E. histolytica to mammalian cells, suggesting its potential as a prophylactic agent against amebiasis.
Review
Biochemistry & Molecular Biology
Yupeng Zhu, Zhengtang Qi, Shuzhe Ding
Summary: The effects of exercise on adipose tissue thermogenesis and browning are controversial. This review discusses factors contributing to conflicting experimental results and explains the impact of cellular redox state on thermogenesis. Furthermore, it proposes a network model in which exercise coordinates adipose tissue browning and thermogenesis through multiple pathways and organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Robyn E. Powell, Martin R. Lees, Graham J. Tizzard, Simon J. Coles, Qingchun Yuan, Petra J. van Koningsbruggen
Summary: The synthesis and crystal structure (100 K) of the compound [(CH3)(2)NH2]-[Fe(C10H11O2N3S)(2)] are reported. The structure consists of an octahedral [Fe-III (L)(2)](-) fragment and a dimethylammonium cation. Each L2- ligand binds to the FeIII ion through the S, N, and O atoms, forming an (FeS2N2O2)-S-III chromophore. The ligands are arranged in two perpendicular planes, with the O and S atoms in cis positions and the N atoms in trans positions. The magnetic measurements confirm the presence of a high-spin Fe-III ion with D = 0.83 (1) cm(-1) and g = 2 at 100 K.
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2023)
Article
Chemistry, Organic
Enrico Podda, Massimiliano Arca, Anna Pintus, Valentina Demontis, Vito Lippolis, Giulio Ferino, James B. B. Orton, Simon J. J. Coles, Maria Carla Aragoni
Summary: 2,7-bis(pyridin-3-ylethynyl)fluoren-9-one [(3-PyE)(2)FO] was synthesized via a one-step Sonogashira coupling reaction between 3-ethynylpyridine and 2,7-dibromofluoren-9-one. The compound was fully characterized and its crystal structure was determined using single-crystal XRD analysis.
Article
Crystallography
Seynabou Sokhna, Insa Seck, Ibrahima El Hadj Thiam, Marc Presset, Samba Fama Ndoye, Lalla Aicha Ba, Issa Samb, Simon Coles, James Orton, Matar Seck, Erwan Le Gall, Mohamed Gaye
Summary: Two new heterocyclic 1,2,3-triazenes were synthesized by coupling reactions of 3-aminopyridine with morpholine and 1,2,3,4-tetrahydroquinoline, respectively. The synthesis was characterized by various techniques including NMR, IR, mass spectrometry, and X-ray diffraction. The crystal structures of the compounds reveal interesting molecular arrangements connected by CHN interactions.
ACTA CRYSTALLOGRAPHICA SECTION E-CRYSTALLOGRAPHIC COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Christopher E. E. Elgar, Haleema Y. Y. Otaif, Joseph M. M. Beames, Peter N. N. Horton, Simon J. J. Coles, Andrew J. J. Hallett, Sean P. P. O'Kell, Simon J. A. Pope
Summary: Six disubstituted ligands based upon 2-(2'-pyridinyl/pyrazinyl)quinoline-4-carboxylic acids have been synthesised for use as ancillary chelating ligands in Ir(III) complexes. X-ray crystallography revealed a distorted octahedral geometry and predicted MLCT/LLCT character for the complexes. The complexes exhibited luminescence in the deep red region and emitted from triplet states.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Lydia Sosa Vargas, Simon J. Coles, Graham J. Tizzard, Isabelle Chambrier, Michael J. Cook, Andrew N. Cammidge
Summary: The entire range of porphyrin-phthalocyanine hybrids can be synthesized by treating 1,4-dioctylphthalonitrile with different amounts of MeMgBr. The resulting mixtures can favor specific hybrid structures, with the extreme case being the formation of tetrabenzoporphyrin. The individual macrocycles can be isolated and demetallated to obtain the metal-free compounds. Lead insertion is successful with all hybrids using lead (II) acetate. The crystal structures of the resulting materials can be determined through X-ray diffraction and exhibit unique stacking patterns of cofacial macrocycles linked by bridging lead ions.
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
(2023)
Article
Chemistry, Multidisciplinary
Qiang Chen, Amber L. Thompson, Kirsten E. Christensen, Peter N. Horton, Simon J. Coles, Harry L. Anderson
Summary: This study reported the synthesis of unprecedented β,β-directly linked cyclic zinc porphyrin oligomers, the trimer (CP3) and tetramer (CP4), through Yamamoto coupling of a 2,3-dibromoporphyrin precursor. The three-dimensional structures of CP3 and CP4 were confirmed by various analytical techniques. CP3 exhibited a propeller shape while CP4 had a saddle shape, resulting in different photophysical and electrochemical properties. CP4 also showed affinity for fullerenes, forming complexes with C70 and C60 in toluene solution.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Organic
M. Carla Aragoni, Vito Lippolis, Annalisa Mancini, Anna Pintus, Enrico Podda, James B. Orton, Simon J. Coles, Massimiliano Arca
Summary: 2-(5,6-diphenyl-1,2,4-triazin-3-yl)pyridinium dichloroiodate (I) (1) was synthesized by reacting 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine with ICl in dichloromethane solution. The structural characterization of 1 was performed using SC-XRD analysis, accompanied by elemental analysis, FT-IR, and FT-Raman spectroscopy measurements.
Article
Chemistry, Organic
Enrico Podda, Massimiliano Arca, Anna Pintus, Francesca Meloni, Vito Lippolis, Giulio Ferino, James B. Orton, Simon J. Coles, Maria Carla Aragoni
Summary: Compound 1, known as 2-(2,7-bis(pyridin-3-ylethynyl)fluoren-9-ylidene)malononitrile, was synthesized by reacting 2,7-bis(pyridin-3-ylethynyl)fluoren-9-one with malononitrile in DMSO solution. The structure of 1 was characterized by SC-XRD analysis, and further confirmed by elemental analysis, FT-IR, NMR, and MS measurements.
Article
Chemistry, Multidisciplinary
Xuelin Yao, Heng Zhang, Fanmiao Kong, Antoine Hinaut, Remy Pawlak, Masanari Okuno, Robert Graf, Peter N. Horton, Simon J. Coles, Ernst Meyer, Lapo Bogani, Mischa Bonn, Hai I. Wang, Klaus Muellen, Akimitsu Narita
Summary: The solution synthesis of 8-AGNRs has been demonstrated, showing the characteristics of low band gap and remarkable NIR absorption.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
M. Carla Aragoni, Enrico Podda, Savita Chaudhary, Aman K. K. Bhasin, Kuldip K. Bhasin, Simon J. Coles, James B. Orton, Francesco Isaia, Vito Lippolis, Anna Pintus, Alexandra M. Z. Slawin, J. Derek Woollins, Massimiliano Arca
Summary: The reactivity between two ligands and I-2/Br-2 is discussed and the structures and spectroscopic properties of the compounds are investigated. The study provides insights into the noncovalent bonding interactions in these compounds.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Simon J. Coles, Simon Cooper, Wim T. Klooster, Laura J. McCormick Mc Pherson, Andrew W. G. Platt
Summary: This study investigates the reactions between ceric ammonium nitrate and bidentate phosphine oxides, and reports the crystal structures and thermal stability of the complexes. NMR spectroscopy is used to study the formation of the complexes in solution.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Robert C. Carroll, David C. Harrowven, James E. Pearce, Simon J. Coles
Summary: The utilization of the crystalline sponge method enables the characterization of a novel family of synthetic organic oils. The structural differences and functional group diversity provided by 13 related molecular adsorbates offer a quantitative understanding of the relationship between guest structure, conformation, and intermolecular interactions with neighboring guests and the host framework. This analysis extends to assess the connection between these factors and the quality indicators for molecular structure elucidation.
Article
Chemistry, Organic
Enrico Podda, Massimiliano Arca, Anna Pintus, Vito Lippolis, Giulio Ferino, James B. Orton, Simon J. Coles, Maria Carla Aragoni
Summary: Compound 2,7-Bis(pyridin-4-ylethynyl)-9H-carbazole (1) was synthesized by reacting 4-ethynylpyridine hydrochloride with 2,7-dibromo-9H-carbazole. The compound 1 was fully characterized, and the crystal structure of its monohydrate was determined by single-crystal XRD analysis.
Article
Chemistry, Multidisciplinary
Qiang Chen, Amber L. Thompson, Kirsten E. Christensen, Peter N. Horton, Simon J. Coles, Harry L. Anderson
Summary: In this study, unprecedented β,β-directly linked cyclic zinc porphyrin oligomers, the trimer (CP3) and tetramer (CP4), were synthesized and their three-dimensional structures were confirmed. The different geometries of CP3 and CP4 result in distinct photophysical and electrochemical properties. CP4, with its saddle-shaped structure, serves as a ditopic receptor for fullerenes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Letter
Rheumatology
Jozelio Freire de Carvalho, Steven John Coles, Allain Amador Bueno
RHEUMATOLOGY INTERNATIONAL
(2023)