Article
Biochemistry & Molecular Biology
S. B. Lohan, D. Ivanov, N. Schueler, B. Berger, L. Zastrow, J. Lademann, M. C. Meinke
Summary: UV radiation causes free radical formation, leading to immunological modulations, skin aging, or skin cancer. A critical radical concentration has been identified in UV and visible light regions as a marker for an imbalance in the redox system. This transition point indicates an inversion in the ratio of ROS to LOS, highlighting the importance of stress in detecting this imbalance.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Electrochemistry
Stephan den Hartog, Mohammad Samanipour, H. Y. Vincent Ching, Sabine Van Doorslaer, Tom Breugelmans, Annick Hubin, Jon Ustarroz
Summary: The study validates a method combining electrochemical and electron paramagnetic resonance (EPR) techniques for investigating oxygen reduction reaction (ORR) intermediates at Pt nanoparticles. The results demonstrate the detection and identification of free radicals produced during ORR, providing a functional proof-of-concept for further research. The proposed procedure could enable a more rigorous quantification of free radicals in other electrochemical reactions.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Haleh H. Haeri, Jana Eisermann, Heike Schimm, Anja Buescher, Peter Hoyer, Dariush Hinderberger
Summary: Patients with nephrotic syndrome (NS) experience urinary loss of albumin, and previous studies mainly focused on the glomerular filter without analyzing the molecular properties of albumin. This study compared serum from pediatric NS patients with healthy subjects and commercial albumin, revealing pronounced differences in binding behavior and surface charge of albumin, which could lead to enhanced filtration and direct toxicity to podocytes.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Boris Epel, Joseph P. Y. Kao, Sandra S. Eaton, Gareth R. Eaton, Howard J. Halpern
Summary: Recent advances in instrumentation and molecular probes have made it possible to quantitatively image tissue redox status in vivo. The use of low-frequency electron paramagnetic resonance (EPR) techniques and novel spin probes has led to significant advancements in this field. However, further improvements in spin probes and RS instrumentation are needed to fully realize the potential of redox imaging in tissue.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Chemistry, Multidisciplinary
Subhashis Jana, Eric G. B. Evans, Hyo Sang Jang, Shuyang Zhang, Hui Zhang, Andrzej Rajca, Sharona E. Gordon, William N. Zagotta, Stefan Stoll, Ryan A. Mehl
Summary: Site-directed spin labeling (SDSL) combined with double electron-electron resonance (DEER) spectroscopy has become a powerful technique for studying the structure and conformational dynamics of biomacromolecules. In this study, a novel family of tetrazine-bearing noncanonical amino acids (Tet-v4.0) was genetically encoded and used for specific and quantitative spin labeling of proteins in live cells. The fast reaction rate of Tet-v4.0 with strained trans-cyclooctene (sTCO)-functionalized nitroxides allowed efficient spin labeling of proteins within minutes, even at sub-micromolar concentrations. DEER measurements from intact cells showed distance distributions consistent with those obtained from proteins purified and labeled in vitro. This approach enables structural and functional studies of proteins directly in cells without the need for protein purification.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biochemistry & Molecular Biology
Claudia Kunst, Stephan Schmid, Marlen Michalski, Deniz Tuemen, Jonas Buttenschoen, Martina Mueller, Karsten Guelow
Summary: The human gastrointestinal tract houses a complex microbial community that plays a crucial role in the overall well-being of the body. The gut microbiota produces various metabolites that regulate biological processes, including immune system regulation. Maintaining a balance in the gut microbiome is essential for a stable REDOX equilibrium, as dysbiosis can disrupt this balance and directly impact the immune system and promote inflammation. In this article, we discuss the common reactive oxygen species (ROS), the transition from a balanced REDOX state to oxidative stress, and the role of the microbiota in maintaining REDOX homeostasis and regulating immune responses and inflammation.
Article
Biochemistry & Molecular Biology
Joanna Czwartos, Bernadeta Dobosz, Wiktoria Kasprzycka, Paulina Natalia Osuchowska, Malgorzata Stepinska, Elzbieta Anna Trafny, Jacek Starzynski, Zygmunt Mierczyk
Summary: The research on the effect of nanosecond electromagnetic pulses on human health, especially the formation of free radicals in human cells, is ongoing. This study conducted a preliminary investigation on the impact of a single high-energy electromagnetic pulse on morphology, viability, and free radical generation in human mesenchymal stem cells (hMSC). The results showed that the exposure to the high-energy electromagnetic pulse did not affect the number of free radicals generated or the morphology of hMSC in vitro compared to control samples, as observed through microscopic examination and EPR measurements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Lei Xu, Yuting Wang, Erqun Song, Yang Song
Summary: In this study, PBDE-Qs and PBDE-HQs were synthesized and their nucleophilic and redox properties were investigated. It was found that PBDE-Qs react with GSH leading to neurotoxic effects, while PBDE-HQs do not have this capability. EPR analysis revealed the generation of SQ(center dot-) by PBDE-Qs which contributes to their toxicity, especially in PBDE-Q/HQ compounds with bromine substitution on the quinone ring.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Volodymyr Lushchak, Michael Duszenko, Dmytro Gospodaryov, Olga Garaschuk
Summary: Neural tissue in mammals is a major consumer of oxygen, generating reactive oxygen (ROS) and nitrogen (RNS) species in the brain. These substances play important signaling roles but can also cause damage, particularly during midlife. Understanding the pathways, defense systems, and changes in energy/ROS balance in midlife is crucial for managing neuroinflammation and oxidative stress.
Article
Engineering, Environmental
Guoqiang Zhao, Binbin Wu, Xiaoshan Zheng, Baoliang Chen, Andreas Kappler, Chiheng Chu
Summary: We have discovered an unrecognized tidal source of reactive oxygen species (ROS) and demonstrated the generation of ROS in coastal surface soil during tidal processes. The fluctuation of redox potential and dissolved oxygen concentration follows a similar pattern to the production of ROS.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zainab Hafideddine, Tim Loier, Niels Van Brempt, Sasha De Henau, H. Y. Vincent Ching, Sander Neukermans, Saskia Defosse, Herald Berghmans, Roberta Sgammato, Roy Aerts, Dietmar Hammerschmid, Rani Moons, Tom Breugelmans, Frank Sobott, Christian Johannessen, Wouter Herrebout, Bart P. Braeckman, Luc Moens, Sylvia Dewilde, Sabine Van Doorslaer
Summary: This study characterizes the biophysical properties and expression pattern of Caenorhabditis elegans globin-3 (GLB-3). GLB-3 is expressed in the reproductive and nervous system and plays a crucial role in fertility and motility. It shows unique stability and is involved in (cysteine) redox chemistry.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jeannine Gruene, Vladimir Dyakonov, Andreas Sperlich
Summary: Triplet excited states in organic semiconductor materials and devices are challenging to study, but play crucial roles in OLEDs and OPV devices. The innovative spin-sensitive method of trODMR can distinguish different states and determine their time scales, revealing fundamental differences in electrically and optically induced triplet states.
MATERIALS HORIZONS
(2021)
Article
Biochemistry & Molecular Biology
Rafael Senos Demarco, D. Leanne Jones
Summary: Redox signaling plays an important role in regulating GSC and gamete behavior across different model organisms. Antioxidants can support mammalian spermatogenesis, with potential strategies for enhancing reproductive fitness in humans.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Nobuhiro Suzuki
Summary: Heat stress has a severe impact on plant growth and crop production, so it is urgent to understand the mechanisms underlying the plant's response to heat stress and develop strategies to enhance crop heat tolerance. Heat stress affects the chloroplasts and mitochondria, leading to increased production of reactive oxygen species (ROS) and disruption of photosynthetic and respiratory metabolisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marc Herb, Alexander Gluschko, Michael Schramm
Summary: Reactive oxygen species (ROS) play important roles in cellular processes but can also lead to diseases, their effects depending on cell type and ROS production conditions. Cellular redox balance, like cell pH and calcium levels, is tightly controlled, with evidence showing that cells regulate ROS production by controlling activation and localization factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
David Novak, Milos Mojovic, Aleksandra Pavicevic, Martina Zatloukalova, Lenka Hernychova, Martin Bartosik, Jan Vacek
BIOELECTROCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Aleksandra Pavicevic, Milan Lakocevic, Milan Popovic, Ana Popovic-Bijelic, Marko Dakovic, Milos Mojovic
BIOLOGICAL CHEMISTRY
(2018)
Correction
Biochemistry & Molecular Biology
J. Egea, I. Fabregat, Y. M. Frapart, P. Ghezzi, A. Goerlach, T. Kietzmann, K. Kubaichuk, U. G. Knaus, M. G. Lopez, G. Olaso-Gonzalez, A. Petry, R. Schulz, J. Vina, P. Winyard, K. Abbas, O. S. Ademowo, C. B. Afonso, I. Andreadou, H. Antelmann, F. Antunes, M. Aslan, M. M. Bachschmid, R. M. Barbosa, V. Belousov, C. Berndt, D. Bernlohr, E. Bertran, A. Bindoli, S. P. Bottari, P. M. Brito, G. Carrara, A. I. Casas, A. Chatzi, N. Chondrogiannni, M. Conrad, M. S. Cooke, J. G. Costa, A. Cuadrado, P. My-Chan Dang, B. De Smet, B. Debelec-Butuner, I. H. K. Dias, J. D. Dunn, A. J. Edson, M. El Assar, J. El-Benna, P. Ferdinandy, A. S. Fernandes, K. E. Fladmark, U. Foerstermann, R. Giniatullin, Z. Giricz, A. Gorbe, H. Griffiths, V. Hampl, A. Hanf, J. Herget, P. Hernansanz-Agustin, M. Hillion, J. Huang, S. Ilikay, P. Jansen-Duerr, V. Jaquet, J. A. Joles, B. Kalyanaraman, D. Kaminskyy, M. Karbaschi, M. Kleanthous, L. O. Klotz, B. Korac, K. S. Korkmaz, R. Koziel, D. Kracun, K. H. Krause, V. Kren, T. Krieg, J. Laranjinha, A. Lazou, H. Li, A. Martinez-Ruiz, R. Matsui, G. J. McBean, S. P. Meredith, J. Messens, V. Miguel, Y. Mikhed, I. Milisav, L. Milkovic, A. Miranda-Vizuete, M. Mojovic, M. Monsalve, P. A. Mouthuay, J. Mulvey, T. Muenzel, V. Muzykantov, I. T. N. Nguyen, M. Oelze, N. G. Oliveira, C. M. Palmeira, N. Papaevgeniou, A. Pavicevic, B. Pedre, F. Peyrot, M. Phylactides, G. G. Pircalabioru, A. R. Pitt, H. E. Poulsen, I. Prieto, M. P. Rigobello, N. Robledinos-Anton, L. Rodriguez-Manas, A. P. Rolo, F. Rousset, T. Ruskovska, N. Saraiva, S. Sasson, K. Schroeder, K. Semen, T. Seredenina, A. Shakirzyanova, G. L. Smith, T. Soldati, B. C. Sousa, C. M. Spickett, A. Stancic, M. J. Stasia, H. Steinbrenner, V. Stepanic, S. Steven, K. Tokatlidis, E. Tuncay, B. Turan, F. Ursini, J. Vacek, O. Vanjnerova, K. Valentova, F. Van Breusegem, L. Varisli, E. A. Veal, A. S. Yalcin, O. Yelisyeyeva, N. Zarkovic, M. Zatloukalova, J. Zielonka, R. M. Touyz, A. Papapetropoulos, T. Grune, S. Lamas, H. H. H. W. Schmidt, F. Di Lisa, A. Daiber
Article
Oncology
Tra-Ly Nguyen, Marie-Julie Nokin, Maxim Egorov, Mercedes Tome, Clement Bodineau, Carmelo Di Primo, Laetitia Minder, Joanna Wdzieczak-Bakala, Maria Concepcion Garcia-Alvarez, Jerome Bignon, Odile Thoison, Bernard Delpech, Georgiana Surpateanu, Yves-Michel Frapart, Fabienne Peyrot, Kahina Abbas, Silvia Teres, Serge Evrard, Abdel-Majid Khatib, Pierre Soubeyran, Bogdan I. Iorga, Raul V. Duran, Pascal Collin
Article
Biochemistry & Molecular Biology
Martina Zatloukalova, Milos Mojovic, Aleksandra Pavicevic, Martin Kabelac, Bruce A. Freeman, Michaela Pekarova, Jan Vacek
Article
Chemistry, Organic
Nikola Babic, Fabienne Peyrot
TETRAHEDRON LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Nikola Babic, Maylis Orio, Fabienne Peyrot
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Valentina Grippo, Milos Mojovic, Aleksandra Pavicevic, Martin Kabelac, Frantisek Hubatka, Jaroslav Turanek, Martina Zatloukalova, Bruce A. Freeman, Jan Vacek
Summary: Fatty acid nitroalkenes are endogenously generated products that mediate signaling actions and induce adaptive responses in inflammatory and metabolic diseases. This study focuses on the reduction-oxidation characteristics and stability of specific NO2-FA regioisomers, providing insight into their biophysical properties and potential pharmacological applications.
Article
Clinical Neurology
Ana Veskovic, Dura Nakarada, Aleksandra Pavicevic, Bogomir Prokic, Milka Perovic, Selma Kanazir, Ana Popovic-Bijelic, Milos Mojovic
Summary: The study investigated the interplay between mitochondrial dysfunction, oxidative stress, and blood-brain barrier disruption in AD pathology using EPR spectroscopy in the 5xFAD mouse model. The results showed altered brain redox state, BBB breakdown, ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the model. EPR spin probes were found to be excellent in vivo reporters of neuronal tissue redox state and BBB integrity, highlighting the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases.
CURRENT ALZHEIMER RESEARCH
(2021)
Review
Chemistry, Physical
Fabienne Peyrot, Sonia Lajnef, Davy-Louis Versace
Summary: In order to address economic issues in the past decade, free-radical photopolymerization has made significant progress. Various photoinitiating systems have been developed to enable photopolymerization reactions in the visible or near infrared range. Electron paramagnetic resonance coupled to spin trapping is an invaluable technique for understanding the photochemical mechanisms and characterizing the nature of radicals in free-radical photopolymerization.
Article
Biochemistry & Molecular Biology
Aleksandra Rancic, Nikola Babic, Maylis Orio, Fabienne Peyrot
Summary: This study investigates the stability of three newly synthesized nitroxide candidates with tetraethyl substitution and compares their stability in vivo. The results show that nitroxides with pyrrolidine and pyrroline cores exhibit higher stability compared to isoindoline nitroxide. Metabolite analysis suggests that the transformation pathway of tetraethyl isoindoline nitroxide involves hydrogen abstraction and subsequent modifications by cytochrome P450.
Article
Chemistry, Multidisciplinary
Christine Elian, Benjamin Mourot, Camil Benbouziyane, Jean-Pierre Malval, Sonia Lajnef, Fabienne Peyrot, Florian Massuyeau, Olivier Siri, Denis Jacquemin, Simon Pascal, Davy-Louis Versace
Summary: In this study, a near-infrared-absorbing heptamethine compound incorporating bulky benzo[cd]indole heterocycles was designed to prevent self-aggregation of the dye. Compared to a reference system, the incorporation of the heterocycles resulted in a significantly enhanced photoinitiating reactivity. The researchers achieved a 100% conversion of acrylates C=C bonds even under air conditions by incorporating the near-infrared sensitizer into a three-component system, which allowed for self-regeneration of the photoredox cycle. This cycle was thoroughly investigated by identifying the intermediates using EPR spectroscopy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Organic
Jules Brom, Antoine Maruani, Serge Turcaud, Sonia Lajnef, Fabienne Peyrot, Laurent Micouin, Erica Benedetti
Summary: The study demonstrates the photocatalytic activity of coumarins derived from [2.2]paracyclophane for the reductive cleavage of sulfonamides under light-irradiation. The findings open up new possibilities for the application of pCps in photocatalysis.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Review
Chemistry, Inorganic & Nuclear
Nikola Babic, Fabienne Peyrot
Meeting Abstract
Biochemistry & Molecular Biology
Sonia Lajnef, Fabienne Peyrot, Claude Wolf, Richard J. Naftalin
FREE RADICAL BIOLOGY AND MEDICINE
(2019)