Article
Microbiology
Tamal Dey, Ardhendu Chakrabortty, Aastha Kapoor, Anuja Warrier, Vijaya Lakshmi Nag, Karthikeyan Sivashanmugam, Manoharan Shankar
Summary: Classical and hypervirulent strains of Klebsiella pneumoniae can cause different types of infections. The strain P34 isolated in India shows hypermucoviscosity but lacks known determinants of hypermucoviscosity, suggesting the presence of novel genetic factors.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Xuemei Yang, Xiaoxuan Liu, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Summary: Klebsiella pneumoniae has become a major bacterial pathogen in hospitals, with a high mortality rate. Factors contributing to its virulence are the overproduction of capsular polysaccharide (CPS) and the hypermucoviscosity (HMV) phenotype. This study investigated the functional characteristics of plasmid-borne rmp homologues in clinical hypervirulent K. pneumoniae strains. The results showed that the rmp homologues play a key role in regulating the HMV phenotype and CPS production in K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Binzhi Dan, Heping Dai, Dangui Zhou, Hongfang Tong, Mei Zhu
Summary: The objective of this study was to analyze the epidemiology of hypervirulent Klebsiella pneumoniae (K. pneumoniae) and explore its drug resistance, ESBLs gene, virulence factor, capsular serotype, and biofilm formation. K. pneumoniae strains were isolated from clinical samples and identified using VITEK2 Compact. Antibiotic susceptibility was determined using microbroth dilution test, while ESBLs production and carbapenemase production were detected using specific methods. PCR was used to detect ESBLs genes, virulence factors, and capsular serotypes. Crystal violet staining was used to assess biofilm formation. The study found that ESBLs genes blaTEM, blaSHV, and blaCTX-M were detected in the K. pneumoniae strains, and multiple ESBLs genes were present in most strains. The virulence genes fimH, ybtS, entB, rmpA, aerobactin, allS, and kfu were also commonly found. It was observed that a significant correlation existed between ESBLs-producing strains and biofilm formation. In conclusion, classical K. pneumoniae has a propensity to acquire antibiotic resistance compared to hypervirulent strains. Moreover, genes encoding rmpA, K1 or K2, and kfu were highly associated with hypervirulent K. pneumoniae.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Immunology
X. Renee Bina, Yuding Weng, James Budnick, Mia E. Van Allen, James E. Bina
Summary: Klebsiella pneumoniae is a dangerous bacterium that causes various human diseases and has become a global health threat due to its antibiotic resistance. In this study, the function of the TolC protein in K. pneumoniae was investigated. The findings reveal that TolC plays a pleiotropic role in K. pneumoniae pathobiology, influencing antimicrobial resistance, capsule production, iron homeostasis, adherence to host cells, and virulence. Understanding the multifaceted role of TolC may guide the development of new therapeutic strategies against K. pneumoniae.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Laura A. Mike, Andrew J. Stark, Valerie S. Forsyth, Jay Vornhagen, Sara N. Smith, Michael A. Bachman, Harry L. T. Mobley
Summary: The study identifies a close relationship between capsule biosynthesis and hypermucoviscosity in Klebsiella pneumoniae, with central metabolism playing a key regulatory role. Certain metabolic genes specifically impact only one of these properties, with genes related to the TCA cycle playing a significant role.
Article
Cell Biology
Suyeon Park, Hyunkeun Kim, Kwan Soo Ko
Summary: In hypervirulent Klebsiella pneumoniae strains, tigecycline resistance is associated with decreased virulence due to reduced production of capsular polysaccharides (CPS). Transcriptomic analyses revealed downregulation of ompK35 expression in tigecycline-resistant mutants, which overexpressed ompR and negatively regulated ompK35 expression. Tigecycline-resistant and ompR-overexpressed mutants exhibited reduced hypermucoviscosity and virulence, while deletion of ompR restored these phenotypes in tigecycline-resistant mutants.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Plant Sciences
Idowu J. Adeosun, Itumeleng T. Baloyi, Sekelwa Cosa
Summary: This study evaluated the antibacterial and anti-biofilm activity of selected phytochemical compounds against Klebsiella pneumoniae. Phytol was found to have the highest potential in inhibiting the formation of biofilm and reducing virulence factors.
Article
Infectious Diseases
Si-Ho Kim, Cheon-Hoo Jeon, Hyoung-Tae Kim, Yu Mi Wi
Summary: This study investigated the clinical characteristics of patients with hypermucoviscous Klebsiella pneumoniae (hmvKp) bacteremia from non-hepatobiliary tract infection. The results showed that hmvKp was associated with less frequent drug resistance and metastatic-purulent presentation, but not with clinical outcomes.
Article
Infectious Diseases
Mengxin Xu, Changrui Qian, Huaiyu Jia, Luozhu Feng, Shiyi Shi, Ying Zhang, Lingbo Wang, Jianming Cao, Tieli Zhou, Cui Zhou
Summary: This study reports for the first time the development of CZA resistance and decreased virulence in ST11 CRKP strains during antimicrobial treatment. The findings emphasize the urgency to monitor CZA resistance and adjust anti-infective treatment strategies in a timely manner.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Dina M. Osama, Bishoy M. Zaki, Wafaa S. Khalaf, Marwa Yousry A. Mohamed, Mahmoud M. Tawfick, Heba M. Amin
Summary: This study investigated the phenotypic and genotypic characteristics of hypermucoviscous Klebsiella pneumoniae (hmvKp) isolated from healthy individuals' gut. All isolates were multidrug-resistant (MDR), and the majority of hmvKp isolates belonged to capsular serotype K2. Multiple virulence genes were detected in all study isolates. The absence of essential hypermucoviscosity-related genes such as magA and rmpA suggests the complex nature of this trait. Further research is warranted to confirm the hypermucoviscosity-related virulence factors among pathogenic and commensal Kp in different colonization niches.
Article
Genetics & Heredity
Mi Nguyen-Tra Le, Shizuo Kayama, Kelly L. Wyres, Liansheng Yu, Junzo Hisatsune, Masato Suzuki, Koji Yahara, Tsuneko Terachi, Kana Sawa, Shin Takahashi, Toshihiko Okuhara, Kunihiko Kohama, Kathryn E. Holt, Tetsu Mizutani, Hiroki Ohge, Motoyuki Sugai
Summary: Klebsiella pneumoniae (Kp) is a global life-threatening pathogen due to its multidrug resistance and hypervirulence phenotype. A study in Japan revealed that the hypermucoviscosity (HMV) phenotype of Kp is influenced by temperature, with strains carrying rmpA/rmpA2 genes showing HMV phenotype at 37 degrees C, while strains negative for these genes tend to show HMV phenotype at room temperature. Hypervirulent Kp clusters carrying rmpA/rmpA2 without ESBL/carbapenemases produced longer string lengths at 37 degrees C and were mostly isolated from the respiratory tract. Other HMV strains, not carrying rmpA/rmpA2 but positive for ESBL/carbapenemases, showed shorter string lengths at 37 degrees C and were frequently isolated from bloodstream infections.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Lijun Wang, Xueting Huang, Qian Jin, Jie Tang, Hua Zhang, Jing-Ren Zhang, Hui Wu
Summary: This study investigates the role of response regulators in mediating hypermucoviscosity in Klebsiella pneumoniae infections. The findings reveal that the response regulator OmpR is essential for hypermucoviscosity and virulence of K. pneumoniae, and its regulation involves energy production and metabolism.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Ahalieyah Anantharajah, Matthieu Deltombe, Marie de Barsy, Stephanie Evrard, Olivier Denis, Pierre Bogaerts, Marie Hallin, Veronique Yvette Miendje Deyi, Denis Pierard, Peggy Bruynseels, Jerina Boelens, Youri Glupczynski, Te-Din Huang
Summary: This study investigated 22 clinical invasive isolates of Klebsiella pneumoniae and found that hypervirulent strains showed increased pathogenicity. The majority of the isolates expressed the K2 capsular serotype and belonged to various MLST lineages, while the remaining strains expressed K1 and were associated with liver abscesses. Among the patients, 36% had primary extra-hepatic infections, 95% had sepsis, and the mortality rate was 30%. Improved clinical and microbiological diagnostics are needed to better understand and manage community-acquired invasive infections caused by hypervirulent K. pneumoniae.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Microbiology
Md Roushan Ali, Yu Yang, Yuanyuan Dai, Huaiwei Lu, Zhien He, Yujie Li, Baolin Sun
Summary: This study conducted molecular and phenotypic analysis on multidrug-resistant hypervirulent K. pneumoniae (MDR-hvKp), revealing two isolates lacking mucoid regulators and virulence factors. The genetic and phenotypic features of these isolates were described, providing important insights into the identification of MDR-hvKp isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Dakang Hu, Yuming Li, Ping Ren, Dongxing Tian, Wenjie Chen, Pan Fu, Weiwen Wang, Xiaobin Li, Xiaofei Jiang
Summary: The positive rates of virulence genes in Klebsiella pneumoniae vary significantly, with iucA, p-rmpA2, and p-rmpA being the primary genes leading to Hv-bla (KPC)(+)-KP. The formation of Hv-bla (KPC)(+)-KP is mostly due to bla (KPC)(+)-KP acquiring another plasmid carrying virulence genes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)