期刊
FRONTIERS IN IMMUNOLOGY
卷 7, 期 -, 页码 1-14出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2016.00034
关键词
oral vaccines; inactivated vaccines; Salmonella typhimurium; Yersina enterocolytica; IgA
类别
资金
- SNF Ambizione fellowship [PZ00P3_136742]
- ETH Research grant [ETH-33 12-2]
- Swiss National Science Foundation [310030_138452]
- European Research Council under the European Union Seventh Framework Program (FP), ERC [281904]
- Swiss National Science Foundation (SNF) [PZ00P3_136742, 310030_138452] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [281904] Funding Source: European Research Council (ERC)
Our mucosal surfaces are the main sites of non-vector-borne pathogen entry, as well as the main interface with our commensal microbiota. We are still only beginning to understand how mucosal adaptive immunity interacts with commensal and pathogenic microbes to influence factors such as infectivity, phenotypic diversity, and within-host evolution. This is in part due to difficulties in generating specific mucosal adaptive immune responses without disrupting the mucosal microbial ecosystem itself. Here, we present a very simple tool to generate inactivated mucosal vaccines from a broad range of culturable bacteria. Oral gavage of 10(10) peracetic acid-inactivated bacteria induces high-titer-specific intestinal IgA in the absence of any measurable inflammation or species invasion. As a proof of principle, we demonstrate that this technique is sufficient to provide fully protective immunity in the murine model of invasive non-typhoidal Salmonellosis, even in the face of severe innate immune deficiency.
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