期刊
BIOENGINEERED
卷 8, 期 5, 页码 524-535出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2016.1257449
关键词
Aldehyde reductase; ethanol production; neutral trehalase; Saccharomyces cerevisiae; Trehalose; Trehalose-6-phosphate synthase
资金
- Ministry of Science and Technology, R.O.C. Taiwan [NSC 100-2313-B-126-001-MY3]
Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30mM furfural and 30mM 5-hydroxymethylfurfural (HMF).
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