Article
Biotechnology & Applied Microbiology
Haddad Antonia-Nancy, Yuto Iwatsuki, Kohei Yabuuchi, Shogo Aso, Toru Katsumata, Keisuke Fukumoto, Yurika Tanaka, Takashi Nakai, Tsuyoshi Shimoboji, Michiaki Matsumoto, Yoshiro Tahara
Summary: This study investigates the potential of CHHA nanogels to protect antibodies from heat denaturation. The results show that CHHA nanogels can not only maintain the activity of antibodies, but also increase their activity. Therefore, CHHA nanogels are a promising method for stabilizing antibodies.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Haofang Zhu, Bin Kong, Junyi Che, Yuanjin Zhao, Lingyun Sun
Summary: This study proposes a bioinspired cationic peptide dendrimer nanogel with DNase I conjugation for the treatment of rheumatoid arthritis. These nanogels exhibit high biocompatibility and have the fastest targeting capability, highest accumulation amounts, longer persistence time, and superior DNA scavenging capacity in inflamed joints.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biotechnology & Applied Microbiology
Huimin Wang, Hong Deng, Menghan Gao, Weiqi Zhang
Summary: This article summarizes the construction of self-assembled NG from natural PS such as chitosan, alginate, and hyaluronan, and introduces their advantages in drug delivery applications. The residues such as amine, carboxyl, and hydroxyl groups on these PS provide multiple sites for both ionic cross-linking and metal coordination, greatly facilitating the formation of self-assembled NG and drug loading, enabling a wide range of biomedical applications for PS NG, especially in drug delivery.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Dingwen Shi, Hongsen Zhang, Haining Zhang, Ling Li, Suping Li, Yanbing Zhao, Chuansheng Zheng, Guangjun Nie, Xiangliang Yang
Summary: In this study, TF-Nanogels, formed by entrapping tTF-pHLIPs into PIB nanogels, exhibited a synergistic effect, enhancing the anti-tumor efficacy of trans-arterial embolization.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Polymer Science
Yagmur Arslan, Alessia Paradiso, Naz Celiktas, Tolga Erdogan, Ozlem Yesil-Celiktas, Wojciech Swieszkowski
Summary: Various patterns exist in nature and can be utilized in tissue engineering through self-organization. The decellularization of plant tissues provides a promising method for tissue regeneration. However, the functionalization and biofabrication strategies of these structures still need improvement.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Parisa Pedram, Claudia Mazio, Giorgia Imparato, Paolo A. Netti, Aurelio Salerno
Summary: Porous microscaffolds play a crucial role in tissue engineering by controlling cell functions and guiding tissue formation. The study developed a new method to prepare porous PCL microscaffolds with a bioinspired structure, supporting the growth and biosynthesis of human dermal fibroblasts. By adjusting PEO concentration, coagulation bath temperature, and starting solution composition, the microscaffolds showed controllable diameter and interconnected pores, enabling the formation of hybrid tissue constructs in vitro.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Nunzio Cennamo, Francesco Arcadio, Fiore Capasso, Devid Maniglio, Luigi Zeni, Alessandra Maria Bossi
Summary: The combination of non-specific deformable nanogels and plasmonic optical probes provides an innovative solution for specific sensing using a generalistic recognition layer. By grafting soft polyacrylamide nanogels onto a gold plasmonic D-shaped plastic optical fiber probe, the researchers were able to optically monitor solvent-to-phase transitions and classify solvents based on refractive index variations. This research has significant implications for the development of smart intelligent sensing processes.
Article
Engineering, Environmental
Xi Ding, Wantao Zhuge, Yue Zhang, Shijie Ding, Jie Wang, Guanghong Zhou
Summary: The simulation of traditional meat characteristics is critical to cultured meat production. In this study, bioinspired microfibers with solidified alginate shells and porcine muscle stem cell (PMSC) loaded hydrogel cores were proposed. The core-shell structure not only provided a spatially confined environment for directional cell alignment, but also facilitated cell migration and mutual fusion. The myogenic differentiation and muscle protein synthesis of PMSCs in microfibers were significantly promoted, resulting in the construction of cultured meat with similar appearance, texture, and protein composition to native pork. By integrating microfluidics and 3D printing technologies, the microfibers were assembled into bulk cultured meat tissues, and simultaneous printing of multiple pieces of cultured meat was achieved.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Vardhman Kumar, David Kingsley, Sajeeshkumar Madhurakkat Perikamana, Pankaj Mogha, C. Rory Goodwin, Shyni Varghese
Summary: This study presents a microfluidic-assisted tissue model of nociception with microvasculature. The neurons and endothelial cells displayed distinct morphologies in the presence of each other. The neurons exhibited an elevated response to capsaicin in the presence of vasculature, and increased TRPV1 receptor expression was observed in the neurons. The platform could also be used to study pain associated with tissue acidosis and pave the way for the development of innervated microphysiological models.
Article
Materials Science, Biomaterials
Jing Liu, Lina Liu, Siheng Li, Qingyun Kang, Ran Zhang, Zhiling Zhu
Summary: This study developed self-assembled nanogels of thiolated silver nanoclusters and chitosan with desirable biocompatibility, targeting specificity, and luminescent properties, showing amplified luminescence and exceptional bactericidal activity compared to individual components. The nanogel also served as a bacterial detection system based on bacterial binding on fluorescent nanogels, promising practical applications as theranostic nanomedicines.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Biology
Hossein Omidian, Renae L. Wilson, Niloofar Babanejad
Summary: Bioinspired polymers have become a promising field in biomaterials research, offering innovative solutions for various applications in biomedical engineering. This manuscript provides an overview of the advancements and potential of bioinspired polymers in tissue engineering, regenerative medicine, and biomedicine, discussing their role in enhancing mechanical properties, mimicking the extracellular matrix, incorporating hydrophobic particles for self-healing abilities, and improving stability. The manuscript also explores their applications in antibacterial properties, optical and sensing applications, cancer therapy, and wound healing, highlighting the transformative impact of bioinspired polymers in biomedical engineering and setting the stage for further research and development in the field.
Article
Materials Science, Biomaterials
Yu-Fon Chen, Yee-Hsuan Chiou, Yi-Cheng Chen, Yi-Sheng Jiang, Ting-Yuan Lee, Jeng-Shiung Jan
Summary: The study created NET-like structures by using DNA and zinc oxide nanoparticles to inhibit inflammation caused by bacterial infections, suggesting a potential therapeutic strategy for bacterial infections.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Pezhman Mohammadi, Julie-Anne Gandier, Nonappa, Wolfgang Wagermaier, Ali Miserez, Merja Penttila
Summary: Inspired by the dactyl club of the stomatopod, a mineralized biocomposite with high strength, stiffness, and fracture toughness is designed and produced using cellulose nanocrystals and genetically engineered proteins. The material's structural properties are controlled through self-assembly and phase separation of protein components, offering an innovative platform for the synthesis of multifunctional biocomposites.
ADVANCED MATERIALS
(2021)
Article
Biophysics
Deepak Patil, Sushma Kumari, Kaushik Chatterjee
Summary: This study presents an innovative spin-coating setup to generate nanotopography on 3D-printed polymer scaffolds, which exhibit excellent bactericidal and osteogenic activities. The nanostructured PLA surface showed a high bactericidal effect on P. aeruginosa and S. aureus, while also supporting pre-osteoblast attachment and proliferation. This work has important implications for engineering next-generation 3D printed bioactive tissue scaffolds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Biomedical
Gaston A. Otarola, Jerry C. Hu, Kyriacos A. Athanasiou
Summary: This study investigates the use of ion modulation to improve the biomechanical properties of engineered neotissues. By manipulating iron and calcium ions, the study achieves tissue maturation and significant improvement in mechanical properties. This has the potential to reduce the reliance on bioreactors and create hyaline-like tissues.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Seiichi Funamoto, Yoshihide Hashimoto, Akio Kishida, Jun Negishi
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2019)
Article
Polymer Science
Masaki Watanabe, Yoshihide Hashimoto, Tsuyoshi Kimura, Akio Kishida
Article
Biochemistry & Molecular Biology
Mako Kobayashi, Junpei Kadota, Yoshihide Hashimoto, Toshiya Fujisato, Naoko Nakamura, Tsuyoshi Kimura, Akio Kishida
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Materials Science, Biomaterials
Yongwei Zhang, Kwangwoo Nam, Tsuyoshi Kimura, Pingli Wu, Naoko Nakamura, Yoshihide Hashimoto, Seiichi Funamoto, Akio Kishida
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Dentistry, Oral Surgery & Medicine
Masaki Watanabe, Hanako Maeda, Yoshihide Hashimoto, Tsuyoshi Kimura, Akio Kishida
DENTAL MATERIALS JOURNAL
(2020)
Article
Cell & Tissue Engineering
Kotaro Yanagisawa, Seiichi Funamoto, Yoshihide Hashimoto, Jun Negishi
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Multidisciplinary Sciences
Mako Kobayashi, Masako Ohara, Yoshihide Hashimoto, Naoko Nakamura, Toshiya Fujisato, Tsuyoshi Kimura, Akio Kishida
Summary: The research investigated the effects of luminal surface structure of decellularized aorta on thrombus formation and cell behavior. The study found that the closer the luminal surface structure resembles the native aorta, the stronger the anti-coagulant property. Vascular cells were able to recognize the luminal surface structure and regulate functional expression accordingly.
Article
Multidisciplinary Sciences
Shunji Kurokawa, Yoshihide Hashimoto, Seiichi Funamoto, Kozue Murata, Akitatsu Yamashita, Kazuhiro Yamazaki, Tadashi Ikeda, Kenji Minatoya, Akio Kishida, Hidetoshi Masumoto
Summary: In this study, xenogeneic implantation of decellularized vascular grafts using high hydrostatic pressure (HHP) was conducted, demonstrating successful graft patency, preservation of extracellular matrix (ECM), and recellularization after implantation.
Article
Materials Science, Multidisciplinary
Tsuyoshi Kimura, Rino Tokunaga, Yoshihide Hashimoto, Naoko Nakamura, Akio Kishida
Summary: The study involved designing and synthesizing an implantable anti-CD25 antibody-immobilized polyethylene (CD25-PE) mesh to suppress tumor growth by capturing regulatory T cells (Tregs). The results showed that the CD25-PE mesh effectively captured CD25-positive cells and demonstrated potential in inhibiting tumor growth in a limited tumor-bearing mouse model. Further research is needed to explore the potential of using implantable anti-CD25 antibody-immobilized material for cancer immunotherapy as a Treg-capturing device.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2021)
Article
Materials Science, Biomaterials
Yoshihide Hashimoto, Akitatsu Yamashita, Jun Negishi, Tsuyoshi Kimura, Seiichi Funamoto, Akio Kishida
Summary: Postoperative adhesions are a common complication in cardiac surgery, and an effective anti-adhesion material is desirable. In this study, an anti-adhesion material was developed using decellularized bovine pericardia and polyethylene glycol, showing superior anti-adhesion effects in a rabbit heart adhesion model. This material is suitable for postoperative care of cardiac surgical patients.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Polymer Science
Mika Suzuki, Tsuyoshi Kimura, Yukina Yoshida, Mako Kobayashi, Yoshihide Hashimoto, Hironobu Takahashi, Tatsuya Shimizu, Shota Anzai, Naoko Nakamura, Akio Kishida
Summary: This study aimed to develop a 3D reconstruction method using recellularized pericardium to overcome difficulties in cell infiltration into tight and dense tissues. Decellularized pericardial tissues were fabricated using high hydrostatic pressurization and surfactant methods, and the mechanical properties were maintained. NIH3T3, C2C12, and mesenchymal stem cells showed dependence on cell type and decellularization method for adhesion, elongation, and alignment. Cultured recellularized pericardium maintained a cylindrical formation and cellular alignment, indicating its potential as an alternative to living tissues.
Article
Biochemistry & Molecular Biology
Mako Kobayashi, Naoki Ishida, Yoshihide Hashimoto, Jun Negishi, Hideki Saga, Yoshihiro Sasaki, Kazunari Akiyoshi, Tsuyoshi Kimura, Akio Kishida
Summary: Research has shown that matrix-bound nanovesicles (MBVs) found in decellularized tissues may play a significant role in tissue remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Mika Suzuki, Tsuyoshi Kimura, Yuta Nakano, Mako Kobayashi, Masahiro Okada, Takuya Matsumoto, Naoko Nakamura, Yoshihide Hashimoto, Akio Kishida
Summary: Recent applications of decellularized tissues include the use of sheets and powders to reconstruct three-dimensional tissue. This study focused on the preparation of mineralized decellularized pericardium for constructing a soft-hard interregional tissue. The findings showed that the decellularization method strongly affects the structure and mineralization of the decellularized pericardium.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Multidisciplinary Sciences
Wen-Jin Ho, Mako Kobayashi, Kozue Murata, Yoshihide Hashimoto, Kenji Izumi, Tsuyoshi Kimura, Hideo Kanemitsu, Kazuhiro Yamazaki, Tadashi Ikeda, Kenji Minatoya, Akio Kishida, Hidetoshi Masumoto
Summary: Decellularized xenogeneic vascular grafts can be used in revascularization surgeries. The application of decellularization methods using high hydrostatic pressure and endothelialization with human endothelial cells can help prevent clot formation and achieve sufficient ex vivo endothelialization, indicating the potential for clinical use.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Biomaterials
Mako Kobayashi, Masako Ohara, Yoshihide Hashimoto, Naoko Nakamura, Toshiya Fujisato, Tsuyoshi Kimura, Akio Kishida
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
