Article
Biotechnology & Applied Microbiology
Monika Tolgo, Silvia Huttner, Peter Rugbjerg, Nguyen Thanh Thuy, Vu Nguyen Thanh, Johan Larsbrink, Lisbeth Olsson
Summary: The study sequenced and analyzed the genome and transcriptome of the thermophilic Ascomycete Thielavia terrestris strain LPH172, revealing a rich arsenal of CAZymes and auxiliary activity (AA) domains. Upregulation of different encoding genes on different substrates suggests a complex and nuanced degradation strategy employed by the fungus.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agronomy
Tereza Hammerschmiedt, Jiri Holatko, Marek Sudoma, Antonin Kintl, Jan Vopravil, Pavel Ryant, Petr Skarpa, Maja Radziemska, Oldrich Latal, Martin Brtnicky
Summary: This study found that the use of biochar-enriched digestate significantly increased soil microbial biomass, soil C:N ratio, fresh above ground biomass, and root biomass. Elemental sulphur-enriched digestate resulted in the highest arylsulfatase and phosphatase, increased urease, microbial biomass in soil, and root biomass. The amendment of digestate + biochar + sulphur led to the significantly highest total soil carbon, microbial biomass, fi-glucosidase, urease, and increased C:N ratio, arylsulfatase in soil, and root biomass, mitigating the adverse effect of either biochar or elemental sulphur on soil respiration. Properties of digestates were apparently affected by pre-incubation, and this approach in digestate fertilizer production may contribute to sustainable farming.
Article
Biotechnology & Applied Microbiology
Ahmad Homaei, Fatemeh Izadpanah Qeshmi
Summary: The hot water of Genow in the central district of Bandar Abbas, Hormozgan Province, Iran, is a rich source of thermophilic protease-producing bacteria, particularly in sediment samples. The purified enzyme BsHR02Pro showed suitable properties for potential applications in various industries.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Samaila Boyi Ajeje, Yun Hu, Guojie Song, Sunday Bulus Peter, Richmond Godwin Afful, Fubao Sun, Mohammad Ali Asadollahi, Hamid Amiri, Ali Abdulkhani, Haiyan Sun
Summary: The conversion of lignocellulose into monosaccharides is crucial for biofuel production. Enzymatic degradation is efficient and can lead to the production of valuable industrial enzymes, but challenges remain in the commercial production of thermostable cellulases and xylanases. The review focuses on recent advancements in isolating, manufacturing, and enhancing the activity of these enzymes from thermophilic and hyperthermophilic microbes using genetic engineering for economic production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Aditya Bhalla, Jessie Arce, Bryan Ubanwa, Gursharan Singh, Rajesh K. Sani, Venkatesh Balan
Summary: A thermophilic Geobacillus bacterial strain WSUCF1 was found to contain carbohydrate-active enzymes capable of hydrolyzing hemicellulose in lignocellulosic biomass. The abundance and activity of these enzymes varied depending on the substrate and conditions used. Combining WSUCF1 secretome with commercial enzymes resulted in efficient saccharification of pretreated corn stover.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Nisha Singh, Anshu S. Mathur, Ravi P. Gupta, Colin J. Barrow, Deepak K. Tuli, Munish Puri
Summary: Thermophilic anaerobic bacteria can produce a variety of lignocellulose degrading enzymes with unique structural features and different biomass degradation schemes. The focus of research is on exploring the opportunities and technological challenges of these enzyme systems in the biofuel industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Energy & Fuels
Anica Dadwal, Shilpa Sharma, Tulasi Satyanarayana
Summary: This study attempts to develop an enzyme cocktail for the conversion of agricultural residues to ethanol. By utilizing recombinant enzymes and optimizing pretreatment methods, high saccharification rate and ethanol production levels were achieved.
BIOENERGY RESEARCH
(2023)
Article
Microbiology
Keerati Tanruean, Watsana Penkhrue, Jaturong Kumla, Nakarin Suwannarach, Saisamorn Lumyong
Summary: Agricultural wastes can be used as raw material in industrial enzyme production through microbial fermentation, with fungi showing high potential as an effective source. The thermophilic fungus Thermoascus aurantiacus strain SL16W was evaluated for its production of lignocellulolytic enzymes and phytase, with the highest phytase activity found in rice bran and optimal conditions for production including the addition of 0.5% ammonium sulfate. The study demonstrated the potential of using agricultural waste to produce value-added industrial enzymes such as phytase.
Article
Multidisciplinary Sciences
Shima Mohammadi, Hossein Tarrahimofrad, Sareh Arjmand, Javad Zamani, Kamahldin Haghbeen, Saeed Aminzadeh
Summary: In this study, a novel cellulase CelC307 was described, and its structure, catalytic mechanism, and activity were investigated by molecular modeling and experimental verification. It was found that CelC307 exhibited strong thermostability and had potential applications in the field of biotechnology.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Alper Akkaya, Yunus Ensari, Emine Erdogan Ozseker, Ozge Ozsen Batur, Gozde Buyuran, Serap Evran
Summary: The complete enzymatic degradation of lignocellulosic biomass requires the cooperative action of cellulosic, hemicellulosic, and lignolytic enzymes. This study focused on the arabinofuranosidase enzyme encoded in A. acidocaldarius genome, and successfully cloned, expressed, purified, and characterized the enzyme.
Review
Microbiology
Ana Laura Totti Benatti, Maria de Lourdes Teixeira de Moraes Polizeli
Summary: Human population growth, industrialization, and globalization have caused severe climate and environmental crisis. Lignocellulolytic enzymes are proposed as a sustainable alternative to mitigate the impact of human activities on the environment. These enzymes can break down lignocellulosic biomass into different monomer units, enabling bioconversion into various products and applications in diverse industries.
Article
Environmental Sciences
Parisa Ebrahimbabaie, Kimiya Yousefi, John Pichtel
Summary: Water pollution caused by microplastics has become a significant environmental and public health concern, and effective removal methods are essential. Photocatalysis and microbial degradation technologies show promise in transforming microplastics into water-soluble substances. Future research should focus on developing more effective technologies and methods to improve water pollution control.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Chemistry, Applied
Shuhang Zhang, Jingjing Wang, Hong Jiang
Summary: Molasses, a major by-product of sugar industry containing high sugars, can be utilized for microbial production of valuable bioproducts. In recent years, biofuels, polysaccharides, oligosaccharides, organic acids, and enzymes have been successfully produced from molasses through microbial conversion, showing potential applications in food, energy, and pharmaceutical industries.
Article
Environmental Sciences
Atif Khurshid Wani, Nahid Akhtar, Nafiaah Naqash, Farida Rahayu, Djajadi Djajadi, Chirag Chopra, Reena Singh, Sikandar I. Mulla, Farooq Sher, Heloisa Pine Americo-Pinheiro Juliana
Summary: Microplastics (MPs) are pervasive pollutants in the environment, with increasing accumulation in aquatic, marine, and terrestrial ecosystems due to human activities. These MPs, which degrade slowly, pose a threat to biota by increasing the probability of biomagnification of persistent, bioaccumulative, and toxic substances. In order to remediate MP-pollutants, efficient strategies are needed to prevent the leaching of contaminants into water, soil, and eventually into humans. Microorganisms capable of degrading plastic polymers through enzymatic actions have been discovered, and metagenomics is an effective tool for exploring and characterizing these plastic-degrading microbial consortia and enzymes. Combined with metatranscriptomics and metabolomics, metagenomics can identify and select remediation-efficient microbes in their natural habitats. Advances in bioinformatics and sequencing tools enable rapid screening, mining, and prediction of genes involved in polymer degradation. This review provides a comprehensive summary of the growing threat of microplastics globally and emphasizes the role of metagenomics and computational biology in developing effective strategies for MP remediation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Green & Sustainable Science & Technology
Fatima Akram, Ikram ul Haq, Amna Aqeel, Zeeshan Ahmed, Fatima Iftikhar Shah
Summary: Human culture has been exploring the potential of using hyperthermophiles and their enzymes as a bio-competitive alternative to fossil-based fuel resources; research has characterized cellulolytic glycoside hydrolases and discussed engineering techniques to enhance the production and stability of thermostable cellulolytic enzymes.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2021)
Article
Plant Sciences
Vandana Sirotiya, Ankesh Ahirwar, Megha Mourya, Mohd Jahir Khan, Anshuman Rai, Rajeev Kwatra, Anil K. Sharma, Harish, Benoit Schoefs, Justine Marchand, Sunita Varjani, Vandana Vinayak
Summary: This article discusses the production of astaxanthin in microalgae under stress conditions and the possibility of utilizing industrial wastewater. By optimizing techniques and overcoming challenges, large-scale production of valuable biocompounds such as astaxanthin can be achieved, paving the way towards sustainable environmental development.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Varruchi Sharma, Anil K. Sharma, Mukesh Yadav, Nirmala Sehrawat, Vikas Kumar, Sunil Kumar, Ajay Gupta, Pooja Sharma, Sasanka Chakrabarti
Summary: MicroRNAs, small non-coding RNAs, play a significant role in regulating gene expression and have implications in various biological processes. This study presents an overview of miRNA prediction methods and their applications in diagnosing and treating diseases, establishing miRNAs as potential biomarkers for multiple diseases.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Amit Vashishth, Nimisha Tehri, Piyush Tehri, Avinash Sharma, Anil Kumar Sharma, Vineet Kumar
Summary: Phosphorous is an essential macronutrient that actively participates in various metabolic and regulatory activities of animals and humans. However, phytic acid, a known antinutritional substance, has a strong affinity for essential mineral ions and can reduce the bioavailability of phosphorous. Bacterial phytases have been identified as potential solutions for efficient utilization of soil phytate and sustainable phosphorous management. This review explores the applications of bacterial phytases in biofertilizers, phosphorus acquisition, and plant growth promotion, and discusses fermentation-based strategies and future trends.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rippin, Anil Sharma, Vikas Beniwal
Summary: Condensed and hydrolyzable tannins in various fruits can be hydrolyzed by tannase enzyme, resulting in the production of gallic acid and tannase-mediated products with potential therapeutic benefits. Prunus domestica and Plum had the highest phenol and hydrolyzable tannin contents, respectively. The hydrolysis of fruit extracts led to increased antioxidant and antibacterial activities.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Poonam Bansal, Varruchi Sharma, Anil Panwar, Raman Kumar, Aanchal Sharma, Seema Ramniwas, Abhijit Dey, Deepak Chandran, Kuldeep Dhama, Anil K. Sharma
Summary: The emergence of the Omicron variant has posed challenges in containing SARS-CoV-2 infection, but a study suggests that Silybin may be used as a therapeutic agent to effectively inhibit viral entry into human cells by blocking the interaction between the spike protein and the host ACE2 receptor.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2023)
Article
Immunology
Sirwan Khalid Ahmed, Salar Omar Abdulqadir, Rukhsar Muhammad Omar, Ardalan Jabbar Abdullah, Hawre Asaad Rahman, Safin Hassan Hussein, Halkawt Ismail Mohammed Amin, Deepak Chandran, Anil Kumar Sharma, Kuldeep Dhama, Malik Sallam, Harapan Harapan, Nader Salari, Chiranjib Chakraborty, Araz Qadir Abdulla
Summary: The general population in the Kurdistan region of Iraq has a moderate level of knowledge, a neutral attitude, and a moderate level of worry about monkeypox. Factors such as age, gender, religion, education level, and residential area are associated with knowledge, attitude, and worry toward monkeypox.
Article
Green & Sustainable Science & Technology
A. Sharma, S. Kumari, S. Sharma, T. Singh, S. Kumar, A. Thakur, S. K. Bhatia, A. K. Sharma
Summary: Layered double hydroxides, also known as hydrotalcite-like compounds, are fascinating mineral materials with wide-ranging applications in catalysis, adsorbents, plastic, agriculture, and electronics. These materials have gained significant interest due to their ease of preparation, ability to intercalate different types of anions, high thermal stability, and biocompatibility. They also offer tailored acidity and basicity, basal spacing, memory effect, anion exchange capability, and a high surface area, making them versatile for various scientific and technological applications. This review focuses on rational design strategies, production, features, and applications of layered double hydroxides in energy and environmental fields, including their use as catalysts, energy storage components, and corrosion scavenging additives.
MATERIALS TODAY SUSTAINABILITY
(2023)
Review
Engineering, Manufacturing
Sonika Kumari, Varruchi Sharma, Ajay Sharma, Aanchal Sharma, Bikram Jit Singh, Anil Kumar Sharma
Summary: Layered double hydroxides (LDHs) are being extensively studied in recent years due to their composition, reusability, cost effectiveness, and ease of synthesis. These materials are considered as green materials because they are non-toxic, easily obtained, and can be produced at low cost using aqueous medium conditions with less hazardous effluents. The simplified method involves one-pot route synthesis using low cost and abundant chemical precursors under mild reaction conditions. LDHs have attracted attention for their various characteristics such as acidic and basic properties, unique diffusion properties, ion-exchangeability, self-healing, and surface modification, making them applicable in energy, environment, catalysis, and biomedical fields. This study focuses on various production methods and their applicability in the biomedical field as delivery agents of bioactive molecules.
INTERNATIONAL JOURNAL OF INTERACTIVE DESIGN AND MANUFACTURING - IJIDEM
(2023)
Review
Biochemistry & Molecular Biology
Nirmala Sehrawat, Mukesh Yadav, Sunil Kumar, Ashwanti Devi, Rajbir Singh, Varruchi Sharma, Kuldeep Dhama, Jose M. Lorenzo, Anil Kumar Sharma
Summary: Cancer remains a major challenge for humans. Researchers have turned their attention to plant-based metabolites, such as those found in mung beans, as a safe and cost-effective strategy for preventing carcinogenesis. Mung beans are nutritionally important legumes that contain bioactive phytochemicals and easily digestible proteins, providing various health benefits. Regular consumption of mung beans can regulate metabolism and have anti-inflammatory, antioxidant, antiproliferative, and immunomodulatory effects, which may help prevent cancer.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Review
Dermatology
Ravi Kant Sharma, Manu Rashmi Sharma, Aneet Mahendra, Ujjawal Sharma, Simranjit Singh, Seema Ramniwas, Anil Kumar Sharma
Summary: Psoriasis is a prevalent chronic inflammatory skin condition caused by abnormal immune response. It not only affects the mental health of patients, but also leads to various systemic diseases. Artificial intelligence can play an important role in the early detection and management of psoriasis.
CURRENT DERMATOLOGY REPORTS
(2023)
Article
Public, Environmental & Occupational Health
Harsh Mohinder Singh, Bikram Jit Singh, Sojan Ritesh, Sojan Baby, Pijush Jaiswal, Anil Kumar Sharma, Rippin Sehgal
Summary: This study examined the relationship between academic stress and somatic symptoms, psychiatric association, demographic and family profile in school going students aged 8-10 years. The findings showed that stress symptoms and psychiatric symptoms were strongly associated with academic stress. The education level of parents and family income were also closely related to academic stress. Additionally, children from low-income families experienced higher academic stress. Previous exam grades and subject failures were inversely related to academic stress. This study highlights the detrimental effects of academic stress on primary school children.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2023)
Article
Environmental Sciences
Usman U. Zango, I. I. Muhammad, Varruchi Sharma, Anil K. Sharma
Summary: The tannery industry generates a large volume of wastewater containing heavy metals and other pollutants, which contaminate water bodies and soil. The use of fungal microbes in bioremediation showed potential in converting these harmful chemical substances into harmless forms. The study found that two fungal isolates, Aspergillus fumigatus and Aspergillus terreus, demonstrated high biosorption abilities individually and as a consortium.
WATER AIR AND SOIL POLLUTION
(2023)
Article
Microbiology
Surya Prakash Sharma, Sujata Dwivedi, Sunil Kumar, Kuldeep Dhama, Anil Kumar Sharma
Summary: Infectious keratitis is a major cause of blindness worldwide. Recent studies have found that contact lens usage, ocular injuries, and ocular surface disease are primary risk factors for keratitis. Advanced imaging techniques and molecular detection methods are more effective than traditional culture methods in rapidly and accurately diagnosing infectious keratitis.
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2023)
Review
Environmental Sciences
Sonika Kumari, Varruchi Sharma, Savita Soni, Ajay Sharma, Abhinay Thakur, Satish Kumar, Kuldeep Dhama, Anil Kumar Sharma, Shashi Kant Bhatia
Summary: Layered double hydroxides (LDHs) are a class of materials with unique structures and properties that have a wide range of applications. LDHs can be synthesized in a cost-effective and environmentally friendly manner through aqueous synthetic pathways, and they have the advantages of easy modification and controlled release. LDHs have great potential in drug delivery, as they can stabilize drugs, enhance solubility, and control release. Additionally, LDHs have good biocompatibility and cellular uptake. This study demonstrates the applications of LDHs and their hybrids/composites in drug, cosmeceutical, and gene delivery systems.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Applied
Varruchi Sharma, Anil Panwar, Atul Sankhyan, Gobind Ram, Anil Kumar Sharma
Summary: COVID-19 has been declared a global pandemic by the WHO, highlighting the urgent need for effective antiviral therapy. Natural compounds, such as Chromone, have shown significant potential in treating COVID-19 and can serve as an adjunctive treatment for SARS-CoV-2 infection.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2023)
