期刊
3 BIOTECH
卷 6, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-016-0396-8
关键词
Anthocyanin; RT-PCR; Spectrophotometer; Transcription factor
资金
- Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea
- Kyungpook National University Research Fund
A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars 'PeachND', 'PeachRed', and 'VividScarlet' under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, andANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.
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