期刊
NUCLEUS
卷 7, 期 1, 页码 27-33出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19491034.2016.1139272
关键词
chromatin organization; diffusion; lamin A; nuclear structure; single particle tracking
类别
资金
- Israel Centers of Research Excellence (ICORE) [1902/12]
- Israel Science Foundation [51/12]
- European Research Council
The organization of the genome in the nucleus is believed to be crucial for different cellular functions. It is known that chromosomes fold into distinct territories, but little is known about the mechanisms that maintain these territories. To explore these mechanisms, we used various live-cell imaging methods, including single particle tracking to characterize the diffusion properties of different genomic regions in live cells. Chromatin diffusion is found to be slow and anomalous; in vast contrast, depletion of lamin A protein significantly increases chromatin motion, and the diffusion pattern of chromatin transforms from slow anomalous to fast normal. More than this, depletion of lamin A protein also affects the dynamics of nuclear bodies. Our findings indicate that chromatin motion is mediated by lamin A and we suggest that constrained chromatin mobility allows to maintain chromosome territories. Thus, the discovery of this function of nucleoplasmic lamin A proteins sheds light on the maintenance mechanism of chromosome territories in the interphase nucleus, which ensures the proper function of the genome.
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