Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

Interleukin-4-Clicked Surfaces Drive M2 Macrophage Polarization

(2016) Tessa Lühmann et al.   CHEMBIOCHEM

Nanotransporters for drug delivery

(2016) Tessa Lühmann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

(2016) Georg Wandrey et al.   Microbial Cell Factories

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates

(2015) Atsushi Yamaguchi et al.   BIOCONJUGATE CHEMISTRY

Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation

(2015) Tessa Lühmann et al.   ACS Biomaterials-Science & Engineering

Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation

(2015) Tessa Lühmann et al.   ACS Biomaterials Science & Engineering

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Unmixing of fluorescence spectra to resolve quantitative time-series measurements of gene expression in plate readers

(2014) Catherine A Lichten et al.   BMC BIOTECHNOLOGY

Decoration of silk fibroin by click chemistry for biomedical application

(2014) Hongshi Zhao et al.   JOURNAL OF STRUCTURAL BIOLOGY

Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation

(2014) Ji Luo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Minireactor-based high-throughput temperature profiling for the optimization of microbial and enzymatic processes

(2014) Martin Kunze et al.   Journal of Biological Engineering

Protein conjugation with genetically encoded unnatural amino acids

(2013) Chan Hyuk Kim et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enhanced phosphoserine insertion duringEscherichia coliprotein synthesis via partial UAG codon reassignment and release factor 1 deletion

(2012) Ilka U. Heinemann et al.   FEBS LETTERS

Efficient Decoding of the UAG Triplet as a Full-Fledged Sense Codon Enhances the Growth of a prfA-Deficient Strain of Escherichia coli

(2012) K. Ohtake et al.   JOURNAL OF BACTERIOLOGY

Genetic-code evolution for protein synthesis with non-natural amino acids

(2011) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Functional context, biosynthesis, and genetic encoding of pyrrolysine

(2011) Marsha A Gaston et al.   CURRENT OPINION IN MICROBIOLOGY

Expanding the Genetic Code of an Animal

(2011) Sebastian Greiss et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Choosing an effective protein bioconjugation strategy

(2011) Nicholas Stephanopoulos et al.   Nature Chemical Biology

Expanding the Genetic Code of Escherichia coli with Phosphoserine

(2011) H.-S. Park et al.   SCIENCE

N6-(2-(R)-Propargylglycyl)lysine as a Clickable Pyrrolysine Mimic

(2010) Xin Li et al.   Chemistry-An Asian Journal

Synthesis of Defined Ubiquitin Dimers

(2010) Silvia Eger et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

(2010) Susan M. Hancock et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

(2009) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteinsviaa Pyrrolysyl-tRNA Synthetase/tRNACUAPair and Click Chemistry

(2009) Duy P. Nguyen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

(2009) Frank Kensy et al.   Microbial Cell Factories

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY