期刊
FRONTIERS IN PHARMACOLOGY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2016.00103
关键词
adenosine A(1) receptor; cGMP; hippocampus; synaptic transmission; soluble guanylyl cyclase; protein kinase G
资金
- FCT [SFRH/BD/65112/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/65112/2009] Funding Source: FCT
Both adenosine A(1) receptor and cGMP inhibit synaptic transmission at the hippocampus and recently it was found that A(1) receptor increased cGMP levels in hippocampus, but the role of cGMP on A(1) receptor-mediated inhibition of synaptic transmission remains to be established. In the present work we investigated if blocking the NOS/sGC/cGMP/PKG pathway using nitric oxide synthase (NOS), protein kinase G (PKG), and soluble guanylyl cyclase (sGC) inhibitors modify the A1 receptor effect on synaptic transmission. Neurotransmission was evaluated by measuring the slope of field excitatory postsynaptic potentials (fEPSPs) evoked by electrical stimulation at hippocampal slices. N6-cyclopentyladenosine (CPA, 15 nM), a selective A(1) receptor agonist, reversibly decreased the fEPSPs by 54 +/- 5%. Incubation of the slices with an inhibitor of NOS (L-NAME, 200 mu M) decreased the CPA effect on fEPSPs by 57 +/- 9% in female rats. In males, ODQ (10 mu M), an sGC inhibitor, decreased the CPA inhibitory effect on fEPSPs by 23 +/- 6%, but only when adenosine deaminase (ADA,1 U/ml) was present; similar results were found in females, where ODQ decreased CPA-induced inhibition of fEPSP slope by 23 +/- 7%. In male rats, the presence of the PKG inhibitor (KT5823, 1 nM) decreased the CPA effect by 45.0 +/- 9%; similar results were obtained in females, where KT5823 caused a 32 +/- 9% decrease on the CPA effect. In conclusion, the results suggest that the inhibitory action of adenosine A1 receptors on synaptic transmission at hippocampus is, in part, mediated by the NOS/sGC/cGMP/PKG pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据