Article
Chemistry, Analytical
Chengxin Wu, Xue Men, Meijun Liu, Yujia Wei, Xing Wei, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: A 2D flow cytometry platform called CytoLM Plus was developed for multi-parameter single-cell analysis. This platform combines fluorescence and side scattering optical detection with ICP-MS detection for a comprehensive analysis of single cells. The performance of CytoLM Plus was evaluated using fluorescent microspheres and cells, and it showed comparable results to a commercial flow cytometer.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Esftathios Kastritis, Ioannis Kostopoulos, Foteini Theodorakakou, Despina Fotiou, Maria Gavriatopoulou, Magdalini Migkou, Maria Irini Tselegkidi, Maria Roussou, Alexandra Papathoma, Evangelos Eleutherakis-Papaioakovou, Ioanna Dialoupi, Nikolaos Kanellias, Argyrios Ntalianis, Pantelis Rousakis, Ioannis P. Trougakos, Ourania Tsitsilonis, Charikleia Gakiopoulou, Evangelos Terpos, Meletios A. Dimopoulos
Summary: Detection of MRD using NGF has significant clinical implications for the treatment and prognosis of AL amyloidosis patients. Patients with undetectable MRD have a higher probability of organ response and a lower probability of hematologic relapse.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2021)
Article
Immunology
Jens Magnus Bernth Jensen, Sune Skeldal, Mikkel Steen Petersen, Bjarne Kuno Moller, Steen Hoffmann, Jens Christian Jensenius, Uffe B. Skov Sorensen, Steffen Thiel
Summary: Research shows that naturally occurring antibodies against terminal galactose-alpha-1,3-galactose play a significant role in protecting against pneumococcal infections by targeting a wide range of microbial polysaccharides. These antibodies constitute a substantial portion of total antibody reactivity in normal human plasma, driving phagocytosis of pneumococci and contributing to human protective immunity on a population level.
Review
Biochemistry & Molecular Biology
Despina Fotiou, Foteini Theodorakakou, Efstathios Kastritis
Summary: Systemic AL amyloidosis is a rare hematological disorder caused by clonal plasma cells producing amyloidogenic immunoglobulins. Prognosis is determined by organ involvement, biology of the plasma cell clone, and response to treatment markers. Current staging systems focus on cardiac dysfunction and renal damage. Discovery of sensitive biomarkers for early diagnosis and comprehensive disease biology assessment is crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Giovanni Palladini, Giampaolo Merlini
Summary: The treatment of AL amyloidosis is a challenge for hematologists, but early diagnosis and personalized therapy can improve patient outcomes.
Article
Pathology
Dragan Jevremovic, Ahmad Nanaa, Susan M. Geyer, Michael Timm, Haya Azouz, Cynthia Hengel, Alexander Reberg, Rong He, David Viswanatha, Mohamad E. Salama, Min Shi, Horatiu Olteanu, Pedro Horna, Gregory Otteson, Patricia T. Greipp, Zhuoer Xie, Hassan B. Alkhateeb, William Hogan, Mark Litzow, Mrinal M. Patnaik, Mithun Shah, Aref Al-Kali, Phuong L. Nguyen
Summary: A simple flow cytometry immunophenotyping (FCIP) approach can identify patients with clonal cytopenia of undetermined significance (CCUS) at higher risk of developing myeloid neoplasia (MN). Flow cytometry immunophenotyping abnormalities, including aberrant CD13/HLA-DR expression pattern at the time of CCUS diagnosis, are significantly associated with the risk of developing MN.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Medicine, General & Internal
Michelle E. Keyel, Kathryn L. Furr, Min H. Kang, C. Patrick Reynolds
Summary: GD2 is present on most neuroblastomas and some other cancers, and anti-GD2 antibodies have potential therapeutic use in neuroblastoma treatment. However, some neuroblastomas are negative for GD2, making it important to identify patients with low GD2 expression for diagnosis and treatment.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Medicine, General & Internal
Kristyna Pekarkova, Jakub Soukup, Marie Kostelanska, Jan Sirc, Zbynek Stranak, Karel Holada
Summary: The study analyzed large EV samples from cord blood using two different thresholding methods and found no difference between the two groups. Despite variations in the numbers of detected lEVs, there was a high correlation between the results obtained through different thresholding methods.
Article
Biochemistry & Molecular Biology
Shanthosh Sivapathan, Paul Geenty, Tejas Deshmukh, Anita Boyd, David Richards, Graeme Stewart, Mark S. Taylor, Fiona Kwok, Liza Thomas
Summary: This study utilized 2D-STE myocardial strain analysis to compare cardiac involvement in AL amyloidosis patients, hypertensive patients, and healthy controls. The results showed significant reductions in global and segmental strain in AL amyloidosis patients, with basal segmental longitudinal strain potentially serving as an early marker of the disease.
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2022)
Article
Microbiology
Sarah M. Yannarell, Eric S. Beaudoin, Hunter S. Talley, Alexi A. Schoenborn, Galya Orr, Christopher R. Anderton, William B. Chrisler, Elizabeth A. Shank
Summary: This study investigated the spatial organization and gene expression relationships for important genes in B. subtilis biofilms, expanding the description of cell populations. The results revealed that only a few subpopulations of cells have specific gene expression, while others exhibit simultaneous expression of multiple genes. These findings provide a framework for further studying and predicting the roles of diverse cellular phenotypes in B. subtilis biofilms.
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Article
Oncology
Karin Ekstrom, Rossella Crescitelli, Hafsteinn Ingi Petursson, Junko Johansson, Cecilia Lasser, Roger Olofsson Bagge
Summary: Lymphatic drainage exudate retrieved from breast cancer patients after surgery contains EVs that can be isolated using SEC isolation. The EVs have several cancer-related markers including CD24, CD29, CD44 and CD146, proteins of potential interest as biomarkers as well as to increase the understanding of the mechanisms of cancer biology.
Review
Pharmacology & Pharmacy
P. S. Russell, R. Velivolu, V. E. Maldonado Zimbron, J. Hong, I. Kavianinia, A. J. R. Hickey, J. A. Windsor, A. R. J. Phillips
Summary: The lymphatic system is increasingly important in various conditions, and fluorescent lymphatic imaging offers advantages over other methods. Successful delivery of fluorescent tracers to lymphatic vessels is a unique challenge due to the one-way drainage system. This review discusses the characteristics of fluorescent tracers and techniques used for high-quality lymphatic imaging, as well as potential areas of future research for improving tracer design.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Clinical Neurology
Hajrullah Ahmeti, Eva Juettner, Christoph Roecken, Olav Jansen, Matthias Laudes, Michael Synowitz
Summary: Pituitary gland metastases are rare and often diagnosed during autopsies. The most common primary tumors that metastasize to the pituitary gland are breast, lung, thyroid, and renal carcinomas. We present a rare case of spindle cell rhabdomyosarcoma metastasizing to the pituitary gland. The patient experienced symptoms such as headache, visual disorder, panhypopituitarism, and diabetes insipidus. Despite the inability to surgically remove the tumor, biopsy confirmed the diagnosis and the patient received chemotherapy and irradiation. Even high-risk patients can achieve remission after treatment.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Review
Cardiac & Cardiovascular Systems
Maria Ungericht, Julia Wanschitz, Alexander S. Kroiss, Christoph Roecken, Thomas Schuetz, Moritz Messner, Marc-Michael Zaruba, Wolfgang N. Loescher, Gerhard Poelzl
Summary: This article describes two patients with ATTR amyloid myopathy, one with ATTR variant amyloidosis (ATTRv) and one with wild-type ATTR amyloidosis (ATTRwt). The ATTRwt patient showed muscular tracer uptake consistent with ATTR amyloid myopathy, while in the ATTRv patient, myopathy occurred after neuropathy and cardiomyopathy. Muscle biopsies confirmed the presence of interstitial amyloid deposits, particularly high in the adipose tissue. This case report highlights the frequent concomitant presence of cardiac ATTR amyloidosis and ATTR amyloid myopathy.
JOURNAL OF NUCLEAR CARDIOLOGY
(2023)
Article
Endocrinology & Metabolism
Darab Ghadimi, Michael Ebsen, Christoph Roecken, Regina Foelster-Holst, Birte Groessner-Schreiber, Christof Doerfer, Wilhelm Bockelmann
Summary: Dietary habits and food can lead to oral dysbiosis, which affects overall health and can lead to systemic diseases. Fermentable carbohydrates in modern diets, such as processed starch and synthetic carbohydrates, are risk factors for oral dysbiosis. The interconnectivity of oral, lung, and gut microbiomes is not well understood, particularly regarding the role of lactobacilli and Lactobacillus phages in rescuing dysbiotic effects.
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS
(2023)
Article
Oncology
Magnus Kock Am Brink, Laura Sophie Dunst, Hans-Michael Behrens, Sandra Krueger, Thomas Becker, Christoph Roecken
Summary: The study investigates the problem of intratumoral heterogeneity (ITH) in gastric cancer (GC), and tests the effects of Ki67 and tumor regression on ITH after neoadjuvant/perioperative chemotherapy. The results suggest that Ki67 is significantly associated with tumor regression and may serve as a predictor for chemotherapy sensitivity and an indicator of ITH.
BRITISH JOURNAL OF CANCER
(2023)
Article
Clinical Neurology
Ulf Jensen-Kondering, Nils G. Margraf, Caroline Weiler, Walter Maetzler, Justina Dargvainiene, Kim Falk, Sarah Philippen, Thorsten Bartsch, Charlotte Flueh, Christoph Roecken, Bettina Moeller, Georg Royl, Alexander Neumann, Norbert Brueggemann, Benjamin Roeben, Claudia Schulte, Benjamin Bender, Daniela Berg, Gregor Kuhlenbaeumer
Summary: This study compared the clinical, radiological, and cerebrospinal fluid marker data of patients with CAA, MLH, AD, and healthy controls. The results showed that cSS was more common in CAA, and MLH with cSS was associated with ICH. The concentrations of cerebrospinal fluid markers differed among the groups.
INTERNATIONAL JOURNAL OF STROKE
(2023)
Article
Oncology
Dita Ulase, Hans-Michael Behrens, Sandra Krueger, Steffen M. Heckl, Ulrike Ebert, Thomas Becker, Christoph Roecken
Summary: This study analyzed the spatial distribution of LAG3 + cells in gastric cancer and its correlation with clinicopathological and survival data. The results demonstrated significant differences in the spatial distribution of LAG3 + cells and their association with prognosis. The findings highlight the importance of LAG3 as a potential prognostic factor and suggest its role in clinical outcomes and treatment responses.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tim A. Steiert, Genis Parra, Marta Gut, Norbert Arnold, Jean-Remi Trotta, Raul Tonda, Alice Moussy, Zuzana Gerber, Peter M. Abuja, Kurt Zatloukal, Christoph Roecken, Trine Folseraas, Marit M. Grimsrud, Arndt Vogel, Benjamin Goeppert, Stephanie Roessler, Sebastian Hinz, Clemens Schafmayer, Philip Rosenstiel, Jean-Francois Deleuze, Ivo G. Gut, Andre Franke, Michael Forster
Summary: Formalin fixation with paraffin-embedding (FFPE) was developed in the late 19th century and is still commonly used in clinical practice. However, FFPE samples can undergo DNA damage, leading to potential errors in sequencing and analysis. This review discusses mitigation strategies in sample quality control, DNA repair treatments, sample preparation, and bioinformatic analysis of FFPE-DNA.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Bente Holm, Stephan Barsuhn, Hans-Michael Behrens, Sandra Krueger, Christoph Roecken
Summary: This study explored the expression and correlation of RNF43 and LRP1B with clinicopathological characteristics in gastric cancer. The expression of RNF43 and LRP1B was significantly correlated with the Lauren phenotype and with each other. Furthermore, differential effects of RNF43 expression were observed between intestinal and diffuse type gastric cancers. These findings suggest that the expression and biological effects of RNF43 and LRP1B in gastric cancer are context-dependent.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Katharina Pries, Sandra Krueger, Steffen Heckl, Hans-Michael Behrens, Christoph Roecken
Summary: Recent studies have found a connection between certain subunits of the SWI/SNF complex and specific tumor characteristics in gastric cancer. This study aimed to examine the expression patterns of SMARCA4 and SMARCE1, two subunits of the SWI/SNF complex, in gastric cancer and their potential relationship with ERBB2. The results showed significant correlations between the expressions of SMARCA4 and SMARCE1 with ARID1A, p53, and microsatellite status, but not with patient prognosis. Amplification analysis revealed co-amplification of SMARCE1 and ERBB2 in some cases, suggesting potential synergistic effects for improved treatment of gastric cancer.
Article
Clinical Neurology
Monika Huhndorf, Christoph Roecken, Charlotte Flueh, Caroline Weiler, Gregor Kuhlenbaeumer, Nora Tegeler, Hannes Schacht, Alexander Neumann, Nils G. Margraf, Ulf Jensen-Kondering
Summary: This study aimed to estimate the frequency of deep-seated microbleeds on MRI in patients with lobar hemorrhages and histopathological evidence for cerebral amyloid angiopathy (CAA). The findings showed that approximately 15% of patients with histopathologically diagnosed CAA have deep-seated microbleeds.
FRONTIERS IN NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Florian Richter, Clara Henssen, Tim Alexander Steiert, Tobias Meissner, Anne-Sophie Mehdorn, Christoph Roecken, Andre Franke, Jan-Hendrik Egberts, Thomas Becker, Susanne Sebens, Michael Forster
Summary: Esophageal cancer (EC) has a high mortality rate, and optimizing therapies and dynamically adapting to individuals is crucial. Liquid biopsy is an increasingly important method for residual disease monitoring, but conflicting detection rates and varying levels of circulating tumor DNA (ctDNA) have been observed in previous studies. This study aims to resolve this discrepancy and found that ctDNA in blood can be used for therapy monitoring of EC patients. A combination of solid and liquid samples should be used to guide individualized EC therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Samir Abdullazade, Hans-Michael Behrens, Sandra Krueger, Jochen Haag, Christoph Roecken
Summary: The study validated gene amplification, protein expression, and tumor biological function of MDM2 in gastric cancer, and found a significant correlation between MDM2 amplification and protein expression. However, MDM2 amplification and expression were not associated with patient survival.
Article
Geriatrics & Gerontology
Patricia Huebbe, Stephanie Bilke, Johanna Rueter, Anke Schloesser, Graeme Campbel, Claus-C. Glueer, Ralph Lucius, Christoph Roecken, Andreas Tholey, Gerald Rimbach
Summary: Recent studies have found that the human APOE epsilon 4 allele protects against non-alcoholic fatty liver disease (NAFLD), while epsilon 3 promotes hepatic steatosis and steatohepatitis. In this study, mice expressing the APOE3 or APOE4 protein isoforms were fed a high-fat and high-sucrose diet to induce obesity. The results showed that APOE3 mice had higher liver weights and exhibited more severe hepatic steatosis compared to APOE4 mice. Proteome analysis also revealed differences in proteins related to inflammation, damage response, and lipid storage between APOE3 and APOE4 mice. These findings support the association of increased NAFLD risk with the APOE epsilon 3 allele and suggest potential mechanisms of protection conferred by APOE epsilon 4.
Article
Medicine, Research & Experimental
Hajrullah Ahmeti, Amke Caliebe, Christoph Rocken, Olav Jansen, Maximilian H. Mehdorn, Michael Synowitz
Summary: This study systematically examined 696 patients with primary intracranial meningiomas to assess the effect of preoperative peritumoral brain edema (PTBE) on preoperative symptoms, neurological deficits, postoperative complications, and long-term outcomes. Results showed that PTBE significantly increased the incidences of specific preoperative symptoms, neurological deficits, and postoperative complications. Patients with preoperative PTBE required more medical support after surgery, but all patients had favorable outcomes.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Hematology
Lukas John, Sandra Sauer, Ute Hegenbart, Peter Dreger, Michael Hundemer, Carsten Mueller-Tidow, Anita Schmitt, Michael Schmitt, Marc S. Raab, Stefan O. Schoenland
Summary: This study assessed the safety and efficacy of donor-derived CAR-T therapy in myeloma patients with prior allo-SCT. The treatment was well tolerated, with no observed adverse effects such as neurotoxicity syndrome or cytokine release syndrome. Importantly, no new graft-versus-host disease was observed, and all patients responded well to treatment.
TRANSPLANTATION AND CELLULAR THERAPY
(2023)