期刊
BIOLOGY OPEN
卷 5, 期 6, 页码 875-882出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.017632
关键词
3D Biology; ECM remodelling; SHG; AFM
类别
资金
- European Research Council [282051]
- European Research Council (ERC) [282051] Funding Source: European Research Council (ERC)
Extracellular matrix (ECM) remodelling is integral to numerous physiological and pathological processes in biology, such as embryogenesis, wound healing, fibrosis and cancer. Until recently, most cellular studies have been conducted on 2D environments where mechanical cues significantly differ from physiologically relevant 3D environments, impacting cellular behaviour and masking the interpretation of cellular function in health and disease. We present an integrated methodology where cell-ECM interactions can be investigated in 3D environments via ECM remodelling. Monitoring and quantification of collagen-I structure in remodelled matrices, through designated algorithms, show that 3D matrices can be used to correlate remodelling with increased ECM stiffness observed in fibrosis. Pancreatic stellate cells (PSCs) are the key effectors of the stromal fibrosis associated to pancreatic cancer. We use PSCs to implement our methodology and demonstrate that PSC matrix remodelling capabilities depend on their contractile machinery and beta 1 integrin-mediated cell-ECM attachment.
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