期刊
BIOLOGY OPEN
卷 5, 期 6, 页码 801-806出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.017384
关键词
Xenopus; Amyloid; Germinal vesicle; Nuclear particles; Oogenesis
类别
资金
- National Institutes of Health [NIGMS grant] [GM069944]
- Internal Research Funding Initiative at University of Iowa
A hallmark of Alzheimer's, Huntington's and similar diseases is the assembly of proteins into amyloids rather than folding into their native state. There is an increasing appreciation that amyloids, under specific conditions, may be non-pathogenic. Here we show that amyloids form as a normal part of Xenopus oocyte development. Amyloids are detectable in the cytosol and the nucleus using an amyloid binding dye and antibodies that recognize amyloid structure. In the cytosol, yolk platelets are amyloid reactive, as are a number of yet to be characterized particles. In the nucleus, we find particles associated with transcription by RNA polymerase I, II and III and RNA processing contain amyloids. Nuclear amyloids remain intact for hours following isolation; however, RNase treatment rapidly disrupts nuclear amyloids.
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