Article
Polymer Science
Aggeliki Stamou, Hermis Iatrou, Constantinos Tsitsilianis
Summary: This study developed PLL-g-NIPAm with LCST thermosensitivity by grafting N-isopropylacrylamide (NIPAm) onto polylysine (PLL), and the LCST is influenced by pH and ionic strength.
Article
Polymer Science
Sun-Jung Yoon, Kyungeun Ohk, Bon Kang Gu, Heung Jae Chun, Dae Hyeok Yang
Summary: This study investigated the hemostatic efficacy of materials based on starch (ST), hyaluronic acid (HA), and poly-L-lysine (PLL). The results showed that ST-HA-PLL samples had the highest hemostasis rate and the lowest hemoglobin-binding efficacy. Histological results indicated that the manufactured samples were cyto-and biocompatible. Therefore, the researchers suggest that these samples have better hemostatic capacity than commercial products.
MACROMOLECULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Liang Wang, Yue Deng, Mengping Wu, Jianhua Zhang, Hongjian Zhang, Zhonggui Mao, Xusheng Chen
Summary: A dynamic pH-regulation strategy was developed to maintain and improve the production of e-poly-L-lysine (e-PL). The strategy effectively increased the production and productivity of e-PL by enhancing key enzyme activity, oxidative phosphorylation, ATP levels, ROS levels, and cellular antioxidant systems.
PROCESS BIOCHEMISTRY
(2022)
Article
Polymer Science
Maria-Eleni Karga, Maria-Eleni Kargaki, Hermis Iatrou, Constantinos Tsitsilianis
Summary: In this study, the rheological behavior of a graft copolymer solution that self-assembles into a hydrogel network was investigated. The copolymer showed strong elasticity and self-healing properties. The stiffness and swelling of the hydrogel could be controlled by adjusting pH conditions. The hydrogel exhibited excellent injectability and instantaneous self-healing, making it a potential candidate for various biomedical applications.
Article
Pharmacology & Pharmacy
Giuseppe Alonci, Roberto Mocchi, Sabrina Sommatis, Maria Chiara Capillo, Elsa Liga, Agata Janowska, Lidia Nachbaur, Nicola Zerbinati
Summary: The study introduces a technology using amino acid L-Lysine as a biocrosslinker to stabilize hyaluronic acid (HA) and produce bionic hydrogels for regenerative medicine applications. These hydrogels exhibit a 3D network structure and mimic biological matrixes, making them a promising candidate for cell proliferation, regeneration, wound healing, and other medical applications.
Review
Chemistry, Multidisciplinary
Sijin Chen, Shuting Huang, Yan Li, Chuncai Zhou
Summary: This review summarized the advanced development in the synthesis and modification strategies of ε-poly-L-lysine (EPL) and lysine-based dendrimer (LBD) compounds, focusing on their applications in biomedical fields such as agents, adjuvants, and carriers for anti-pathogen, tumor, and gene therapies. The combination with other advanced methodologies and technologies shows potential for overcoming challenges like scale production and high expenses in the future development of lysine-based polymers.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Angel Espinoza-Gonzalez, Carmen Hernandez-Valencia, Luis Cedeno-Caero, Roberto Sanchez-Sanchez, Carmina Montiel, Miquel Gimeno
Summary: Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, can catalyze the synthesis of high molecular weight cationic alpha-poly-L-lysine and amphiphilic poly(alpha-L-lysine-co-L-phenylalanine) in organic solvent. The reaction is conducted in hydrophobic non-toxic 1,1,1,2-tetrafluoroethane solvent, and the low water environment limits hydrolysis of the peptide chains. The organic compressed fluid used in the reaction has low-pressure requirements and evaporates completely after the reaction, leaving no solvent residues. The resulting polypeptides show no cytotoxicity in human cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Polymer Science
Maria-Eleni Kargaki, Foteini Arfara, Hermis Iatrou, Constantinos Tsitsilianis
Summary: We investigated the rheological behavior of a graft copolymer polyampholyte composed of polyacrylic acid (PAA) backbone grafted by Poly (L-lysine) (PAA-b-PLL). The hydrogel formed by self-assembly displayed interesting properties such as low critical gel concentration, elastic response, extended critical strain, shear responsiveness, injectability, 3D printability, and self-healing. The properties of the hydrogel were pH-dependent and influenced by the presence of salt due to the changes in PLL secondary structure and crosslinking mode.
Article
Chemistry, Multidisciplinary
Jie Yu, Danwei Cen, Yongcheng Chen, Hailan Zhao, Mengyue Xu, Sulan Wu, Shuo Wang, Qiao Jin, Ting Shen
Summary: Antibiotic resistance poses a significant threat to public health, and improving the therapeutic efficacy of conventional antibiotics is an effective solution. By conjugating the positively charged epsilon-poly-l-lysine to erythromycin, the modified EPL-EM showed enhanced antibacterial activity and reduced drug resistance development. Furthermore, EPL-EM exhibited excellent biocompatibility without inducing hemolysis and cytotoxicity.
Article
Biotechnology & Applied Microbiology
Gavin Gonzales, Jiaul Hoque, Anna Gilpin, Biswanath Maity, Stefan Zauscher, Shyni Varghese
Summary: This study focuses on the development of cationic nanocarriers to overcome the challenges of drug delivery to cartilage tissue. These nanocarriers, which adhere quickly to cartilage tissue and can penetrate and remain within its full thickness, show promising results for therapeutic outcomes.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Applied
Hao Wu, Shuqian Hu, Chunling Nie, Jiahui Zhang, Huafeng Tian, Weicheng Hu, Ting Shen, Jianguo Wang
Summary: A novel method was described for synthesizing antibacterial cellulose beads, which exhibited excellent antimicrobial activity and biodegradability, showing great potential for use in food and environmental applications.
CARBOHYDRATE POLYMERS
(2021)
Review
Biotechnology & Applied Microbiology
Liang Wang, Chongyang Zhang, Jianhua Zhang, Zhiming Rao, Xueming Xu, Zhonggui Mao, Xusheng Chen
Summary: The review compares traditional and advanced strategies for discovering and cultivating high-producing strains of epsilon-poly-L-lysine, including traditional methods based on isolation and culture, genome mining, and directed evolution. Additionally, process engineering methods for improving production and efficient downstream purification methods are discussed in the article.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Chunling Nie, Ting Shen, Weicheng Hu, Qin Ma, Jiahui Zhang, Shuqian Hu, Huafeng Tian, Hao Wu, Xiaogang Luo, Jianguo Wang
Summary: In this study, cellulose beads grafted with epsilon-poly-L-lysine were prepared through chemical reactions. The grafted beads showed strong antibacterial activities and biodegradability, indicating potential applications in water purification and food treatment fields.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Cong Wang, Hui Cao, Lingyun Jia, Wenli Liu, Pengtao Liu
Summary: This study designed an aerogel material using TOCNF/ε-PL network and citric acid as the cross-linking agent. Experimental results showed that the aerogel had good mechanical properties, antibacterial effects, biodegradability, and blood compatibility, indicating great potential applications in medical and food packaging fields.
CARBOHYDRATE POLYMERS
(2022)
Article
Biotechnology & Applied Microbiology
Mingxuan Wang, Chunchi Rong
Summary: Poly(L-diaminopropionic acid) and poly(epsilon-L-lysine) were co-produced using waste biomass and spent mushroom substrate in solid-state fermentation. After optimization, the maximum yield of the two polymers was achieved with reduced fermentation time. The antimicrobial effects of the polymers were evaluated in freshly pressed grape juice.
Article
Nanoscience & Nanotechnology
Marco Schnurbus, Michael Hardt, Pascal Steinforth, Javier Carrascosa-Tejedor, Samuel Winnall, Philipp Gutfreund, Monika Schoenhoff, Richard A. Campbell, Bjoern Braunschweig
Summary: Light can trigger property changes in polyelectrolyte/surfactant mixtures, such as electrophoretic mobilities, particle size, interfacial structure, and foam stability.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Mark P. Rosenwinkel, Monika Schoenhoff
Summary: The addition of a lithium-coordinating polymer can improve the performance of salt-in-ionic liquid electrolytes to IL-based ternary polymer gel electrolytes. With an increase in the polymer fraction, the lithium ion transport mechanism changes, with a sign reversal of Li+ mobility and transference number occurring when the polymer contains 5-6 ethylene oxide units per Li+ ion.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2022)
Article
Chemistry, Applied
Philipp Schroeder, Jasper Wattjes, Monika Schoenhoff, Bruno M. Moerschbacher, Cornelia Cramer, Stefan Cord-Landwehr
Summary: A new method for quantitatively analyzing chitosan in aqueous solution using enzymatic cleavage, N-acetylation, UHPLC separation, and ELSD detection is introduced. The method allows for chromatographic separation solely based on the degree of polymerization. The overall chitosan concentration is determined by multiplying the concentrations of chitooligosaccharides by their degree of polymerization.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Multidisciplinary
Pinchas Nuernberg, Jaschar Atik, Oleg Borodin, Martin Winter, Elie Paillard, Monika Schoenhoff
Summary: By designing organic cations with a Li-coordinating chain, ion-ion correlations in concentrated ionic liquid electrolytes can be exploited to achieve positive Li+ ion mobilities. The novel concept not only corrects the anionic drift direction of Li in ionic liquids but also utilizes strong ion correlations in concentrated electrolytes for superionic transport.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Nicolas Keppeler, Paula D. Galgano, Monika Schoenhoff, Naved I. Malek, Omar A. El Seoud
Summary: This study investigates the properties of a series of ionic liquid-based surfactant halides, including the enthalpy and entropy changes during micelle formation, as well as the solubilization power. The findings suggest that variations in chain length and head-group have significant effects on the properties of the surfactants.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Giselle de Araujo Lima e Souza, Maria Enrica Di Pietro, Franca Castiglione, Pedro Henrique Marques Mezencio, Patricia Fazzio Martins Martinez, Alessandro Mariani, Hanno Maria Schuutz, Stefano Passerini, Maleen Middendorf, Monika Schonhoff, Alessandro Triolo, Giovanni Battista Appetecchi, Andrea Mele
Summary: This study investigates the effects of structural features on the properties of ionic liquids (ILs) and compares the behaviors of different ILs. The results show that the thermal and electrochemical properties of ILs are influenced by the size and asymmetry of the ions, highlighting the importance of tailoring ILs properties for specific applications.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Physical
Martin Lorenz, Franziska Kilchert, Pinchas Nuernberg, Max Schammer, Arnulf Latz, Birger Horstmann, Monika Schoenhoff
Summary: This study uses electrophoretic NMR to reveal that ion transport processes do not follow momentum conservation, but are instead constrained by the conservation of local molar species volumes. The experimentally quantified net volume flux is found to be zero, implying a non-zero local momentum flux, impacting the calculation of transference numbers and comparisons of MD results to experimental findings.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Physical
Pascal Steinforth, Melania Gomez-Martinez, Lukas-Maximilian Entgelmeier, Olga Garcia Mancheno, Monika Schoenhoff
Summary: The anion binding behavior of a tetratriazole host is studied, and the influence of cation structures on the anion binding constant is demonstrated. Electro-phoretic NMR is employed to distinguish between charged and uncharged species and correct the anion binding constant. It is also found that cation aggregation affects the anion binding constant.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Philipp Schroeder, Stefan Cord-Landwehr, Monika Schoenhoff, Cornelia Cramer
Summary: This study investigates the composition and charge compensation of complex coacervates of chitosan and gum arabic, depending on pH and salt concentration. The individual polyelectrolyte yields were determined using thermogravimetric analysis and enzymatic hydrolysis/HPLC-ELSD for chitosan quantification. The mass ratio of polyelectrolytes in the complex coacervate remains constant regardless of pH, with either chitosan or gum arabic charges in excess depending on pH. The amount of extrinsic charge compensation in the complex coacervate is discussed, and the doping level is found to be suitable for describing pH-induced extrinsic charge compensation in such systems.
Article
Polymer Science
Philipp Schroeder, Monika Schoenhoff, Cornelia Cramer
Summary: Complex coacervates of chitosan and gum arabic, two naturally abundant weak polyelectrolytes, were studied using oscillatory shear rheology. The results showed that the shear moduli scaled with temperature, salt concentration, and pH, indicating the applicability of time-temperature superposition (TTSP), time-salt superposition (TSSP), and time-pH superposition (TpHSP). However, a breakdown of superposition occurred in the low-frequency regime at high salt concentrations and pH values near the pK (a) of chitosan. This breakdown was attributed to gelation, likely due to the associative interaction of chitosan chains and the formation of a chitosan network.
Article
Chemistry, Physical
Janis Hessling, Martin Lange, Monika Schoenhoff
Summary: Ionic Liquids (IL) show promise as liquid electrolyte components for Li-ion batteries due to their high electrochemical stability and low volatility and flammability. However, unfavorable Lithium-anion clusters lead to poor Li+ transport properties. Confined ILs in nanoporous materials could overcome these problems by altering their structural and dynamic properties. This study investigates the coordination and dynamics of Li+ in confined IL/Li-salt mixtures and reveals potential effects of confinement on ion clustering in the electrolyte.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Franziska Kilchert, Martin Lorenz, Max Schammer, Pinchas Nuernberg, Monika Schoenhoff, Arnulf Latz, Birger Horstmann
Summary: Transference numbers are crucial for understanding electrolyte dynamics and assessing battery performance. However, measuring these transport parameters in highly concentrated liquid electrolytes like ionic liquids is challenging. Additionally, the interpretation of their sign and magnitude has sparked ongoing debate, complicated by language differences. This study highlights the role of the reference frame and proposes a novel thermodynamically consistent theory for highly correlated electrolytes. The theory emphasizes local volume conservation as a key principle in incompressible liquid electrolytes and suggests using volume-based drift velocity as a reference.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Arthur Lenoch, Monika Schoenhoff, Cornelia Cramer
Summary: This study investigates the mechanical properties of hydrogels with reversible transition metal-polymer crosslinks using rheology and models the corresponding mechanical relaxation during gelation. The study finds that the system transitions from an unentangled chain regime to a crosslink dissociation dominated regime and the relaxation is governed by two timescales with different activation energies. By introducing a time-temperature-superposition procedure, the interplay of chain and crosslinker dynamics can be separated.
Article
Polymer Science
Daniel Schlattmann, Monika Schoenhoff
Summary: This study investigates the phase transition behavior of differently crosslinked PNiPAM/BIS microgels with aromatic additives, and analyzes the influence of model drugs on VPTT. The results show that VPTT increases with crosslinker content, accompanied by a drastic decrease of transition enthalpy. The presence of an additive generally decreases VPTT, but with distinct differences concerning the crosslinker content.
Article
Chemistry, Multidisciplinary
Giada Quintieri, Daniel Schlattmann, Monika Schoenhoff, Andre H. Groeschel
Summary: This study provides guidelines on how to form multicompartment micelles with defined shapes and inner structure through direct redispersion of block terpolymer bulk morphologies in selective plasticizing solvents.