Article
Optics
Zong-Da Zhang, Si-Yu Yin, Li-Cheng Wang, Ying-De Wang, Yun-Fei Li, Zhen-Nan Tian, Qi-Dai Chen
Summary: An array of single NV centers was prepared using the femtosecond laser direct writing method to address the accuracy and consistency issues in static magnetic field detection. The prepared NV centers have fewer defects and good stress uniformity, with an average spatial positioning error of only 0.2 μm. This array enables high accuracy measurement of magnetic field vector and gradient.
Article
Materials Science, Multidisciplinary
Kin On Ho, Man Yin Leung, Yiu Yung Pang, King Cho Wong, Ping Him Ng, Sen Yang
Summary: This study investigates the properties inside polymeric products using a distinctive protocol with the negatively charged nitrogen vacancy center in nanodiamond. By measuring the time dependence of local pressure and strain during the chemical processes, the researchers were able to probe the local shear stress inside the materials in situ. This methodology is applicable to any kind of transparent amorphous solids with stress in the order of MPa and could potentially be applied to nanoscale in situ property studies with better apparatus.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Physics, Multidisciplinary
Tianyu Xie, Zhiyuan Zhao, Maosen Guo, Mengqi Wang, Fazhan Shi, Jiangfeng Du
Summary: Research shows that even under ambient conditions, negatively charged nitrogen-vacancy (NV-) centers in diamond exhibit identical properties at Hz-precision level, offering a method for precision measurements in solids and implying a potential application of an atomiclike clock based on ensemble NV centers.
PHYSICAL REVIEW LETTERS
(2021)
Article
Physics, Applied
M. Haruyama, H. Kato, M. Ogura, Y. Kato, D. Takeuchi, S. Yamasaki, T. Iwasaki, H. Morishita, M. Fujiwara, N. Mizuochi, T. Makino
Summary: In this study, electrical excitation and detection of electroluminescence (EL) of single negatively charged nitrogen vacancy (NV-) centers were achieved using lateral diamond p(+)-i(n(-))-n(+) diodes. The efficiency of the electrical excitation rate of the NV center was significantly enhanced by introducing this diode structure compared to previous vertical diode structures. The charge state of the NV center could be manipulated by the voltage applied to the diode, leading to increased dominance of NV- in the emission of EL.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Physical
Olivia Woodman, Abdolreza Pasharavesh, Christopher Wilson, Michal Bajcsy
Summary: We propose a scheme for detecting single microwave photons by utilizing dipole-induced transparency (DIT) in an optical cavity coupled to a spin-selective transition of a negatively charged nitrogen-vacancy (NV-) defect in diamond crystal lattices. The interaction between the optical cavity and the NV- center is controlled by the microwave photons through the spin state of the defect. The spin state is then measured with high fidelity by counting the number of reflected photons when the cavity is probed by resonant laser light. Numerical simulations show that detection efficiencies of nearly 90% and fidelities exceeding 90% are achievable with realistic cavity parameters.
Article
Biology
Hua Leonhard Tan, Stefanie Bungert-Plumke, Daniel Kortzak, Christoph Fahlke, Gabriel Stoelting
Summary: The oligomeric state of plasma membrane proteins is crucial for their function and can be determined using super-resolution microscopy techniques. The dual-color colocalization (DCC) approach we developed allows for accurate counting of protein subunits and simplifies data processing.
Article
Optics
Y. Shimotsuma, K. Kinouchi, R. Yanoshita, M. Fujiwara, N. Mizuochi, M. Uemoto, M. Shimizu, K. Miura
Summary: The NV centers in a diamond were successfully created using a femtosecond laser single pulse. The effect of different laser pulse widths on the diamond lattice was investigated both experimentally and theoretically. Interestingly, despite the high thermal conductivity of a diamond, a suitable pulse repetition rate of several tens kHz was found for the formation of NV center ensembles by femtosecond laser pulse irradiation.
Article
Nanoscience & Nanotechnology
Chen Yang, Weilin Hu, Xuefeng Guo
Summary: Chirality plays a crucial role in various processes and there is a need for accurate distinction among different chiralities. The article proposes a universal strategy for chirality detection and control by aligning the molecular frame with external stimuli.
NANO-MICRO LETTERS
(2023)
Review
Chemistry, Analytical
Fei Ma, Xinyi Zhang
Summary: MicroRNAs play a crucial role in regulating post-transcriptional gene expression and are involved in various cellular processes. Dysregulation of miRNAs can contribute to the development of human diseases, including cancer. Accurate and sensitive detection of miRNAs is essential for disease diagnosis, prognosis, and therapy. Single-molecule fluorescence detection is an advanced bioanalysis technology that offers simplicity, minimal sample consumption, ultrahigh sensitivity, and rapid assay time. This review summarizes the recent advances in single-molecule/particle counting for miRNA detection and imaging in vitro and in vivo, discussing their principles, features, and biomedical applications. The future directions in this field are also highlighted.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Masanori Fujiwara, Shunsuke Inoue, Shin-ichiro Masuno, Haining Fu, Shigeki Tokita, Masaki Hashida, Norikazu Mizuochi
Summary: Recently, the creation of nitrogen-vacancy (NV) centers using ultrashort laser processing has gained attention for quantum information devices. This study demonstrates the creation of high-density NV centers in a wide region using a single intense femtosecond laser pulse, which is important for quantum sensing applications. The technique allows for the expansion of the region containing NV centers and has the potential to fabricate ultrahigh-sensitivity quantum sensors.
Article
Chemistry, Multidisciplinary
Yan Zhang, Chen Wang, Xiaoran Zou, Xiaorui Tian, Juan Hu, Chun-Yang Zhang
Summary: This enzyme-free single-molecule/particle detection method enables simultaneous detection of multiple lncRNAs in cancer cells with dual-targetability, good selectivity, and high sensitivity, even in single cancer cells. It has the ability to discriminate cancer cells from normal cells, features simple sequence design and enzyme-free, holding great potential in living cell imaging and early clinical diagnosis.
Article
Chemistry, Physical
Yuliya Mindarava, Remi Blinder, Valery A. Davydov, Mustapha Zaghrioui, Viatcheslav N. Agafonov, Cecile Autret, Priyadharshini Balasubramanian, Raul Gonzalez Brouwer, Fedor Jelezko
Summary: The article demonstrates the synthesis of core-shell diamond nanoparticles with nitrogen-vacancy centers and naturally abundant C-13 in the core and highly isotopically enriched shell. The particles were formed by overgrowth on previously electron-irradiated nanodiamonds under high-pressure high-temperature conditions. The research showed promising potential for these particles in transferring spin polarization and hyperpolarization of nuclear spins in a fluid environment.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Multidisciplinary Sciences
Chih-Ping Mao, Shih-Chin Wang, Yu-Pin Su, Ssu-Hsueh Tseng, Liangmei He, Annie A. Wu, Richard B. S. Roden, Jie Xiao, Chien-Fu Hung
Summary: Characterizing individual biomarker protein molecules in patient blood samples using single-molecule imaging techniques holds promise for early disease diagnosis and effective treatment. However, current methods have detection limits that are orders of magnitude higher than needed, making them unsuitable for clinical use. The development of SMAC, with its ability to quantify and characterize protein molecules down to the subfemtomolar range, presents a new avenue for noninvasive disease profiling with single-molecule imaging.
Article
Nanoscience & Nanotechnology
Yoav Ninio, Nir Waiskopf, Idan Meirzada, Yoav Romach, Galya Haim, Shira Yochelis, Uri Banin, Nir Bar-Gill
Summary: Nitrogen-vacancy (NV) color centers in diamond have been proven to be useful magnetic sensors for measuring spin fluctuations and achieving high sensitivity and spatial resolution, enabling exploration of various biological and chemical processes catalyzed by reactive oxygen species (ROS). The novel approach demonstrated in this study allows for measuring and quantifying hydroxyl radicals with high spatial resolution using the fluorescence difference between NV charged states, achieving a sensitivity of 11 +/- 4 nM/root Hz localized to a volume of about 10 picoliters without the need for spin labels.
Article
Chemistry, Analytical
Sujitha Puthukodan, Martina Hofmann, Mario Mairhofer, Hannah Janout, Jonas Schurr, Fabian Hauser, Christoph Naderer, Johannes Preiner, Stephan Winkler, Dmitry Sivun, Jaroslaw Jacak
Summary: Extracellular vesicles (EVs) have immense potential as therapeutic agents and diagnostic tools due to their role in cell-cell communication. This study utilized single-molecule microscopy techniques to comprehensively characterize and measure the uptake of HEK293T cell-derived EVs (eGFP-labeled) in HeLa cells. The analysis revealed that 68% of fluorescently labeled EVs had an average size of 45 nm. 3D colocalization analysis of two-color direct stochastic optical reconstruction microscopy (dSTORM) images showed that 25% of uptaken EVs colocalized with transferrin.
ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Brian R. Patton, Daniel Burke, David Owald, Travis J. Gould, Joerg Bewersdorf, Martin J. Booth
Article
Materials Science, Multidisciplinary
Yaoyang Hu, Benjamin T. Miles, Ying-Lung D. Ho, Mike P. C. Taverne, Lifeng Chen, Henkjan Gersen, John G. Rarity, Charl F. J. Faul
ADVANCED OPTICAL MATERIALS
(2017)
Article
Multidisciplinary Sciences
Benjamin T. Miles, Alexander B. Greenwood, David Benito-Alifonso, Hugh Tanner, M. Carmen Galan, Paul Verkade, Henkjan Gersen
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Multidisciplinary
Chong Chen, Pengcheng Ding, Manuela Mura, Yanghan Chen, Ye Sun, Lev N. Kantorovich, Henkjan Gersen, Flemming Besenbacher, Miao Yu
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Multidisciplinary Sciences
Stephen D. Grant, Kyle Richford, Heidi L. Burdett, David McKee, Brian R. Patton
ROYAL SOCIETY OPEN SCIENCE
(2020)
Article
Chemistry, Multidisciplinary
Alexander B. Greenwood, Krishna C. Balram, Henkjan Gersen
Summary: In this work, evidence of a reactive ion etching process is shown, which selectively etches gold along crystalline facets, leading to smoother fabricated plasmonic devices. Further optimization of the etch chemistry holds the prospect of achieving atomic smoothness, opening up a route toward fabricating high-performance plasmonic circuits.
Article
Chemistry, Analytical
Niall M. C. Mulkerns, William H. Hoffmann, Ian D. Lindsay, Henkjan Gersen
Summary: Capillary-based backscattering interferometry has been extensively studied to measure molecular binding. This study analyzed the fringe patterns and spatial chirps in the backscatter direction, and introduced explicit polarization dependence to improve the existing models. The inner diameter of the capillary was found to be crucial for the effectiveness of dechirping algorithms, suggesting potential improvements for further research.
Article
Chemistry, Analytical
Niall M. C. Mulkerns, William H. Hoffmann, Ian D. Lindsay, Henkjan Gersen
Summary: This study presents a ray tracing model for on-chip backscattering interferometry with a semicircular cross-section. Comparing with experimental data, it is found that the current semicircular geometry cannot explain the results, indicating the need for further clarification on the optical mechanisms behind different modalities of backscattering interferometry.
Article
Quantum Science & Technology
Alexander B. Greenwood, Ruth Oulton, Henkjan Gersen
Summary: New techniques for super resolution imaging have been developed by deconstructing scattered light into several spatial modes, allowing us to overcome 'Rayleigh's curse'. By implementing this approach on high numerical aperture objectives, we show that gold nanoparticles can couple to higher order modes without lateral displacement due to their intrinsic asymmetry. This technique is not only applicable to practical nanoparticles, but also to any dipole emitter with emission asymmetry. Full polarization analysis suggests that spatial mode demultiplexing techniques can be applied.
QUANTUM SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Niall M. C. Mulkerns, William H. Hoffmann, Javier Ramos-Soriano, Noelia de la Cruz, Teodoro Garcia-Millan, Robert L. Harniman, Ian D. Lindsay, Annela M. Seddon, M. Carmen Galan, Henkjan Gersen
Summary: A practical method based on measuring the refractive index of nanoparticles in suspension is proposed for determining the ratio of functionalisation layer volume to particle volume. The method is successfully applied to different nanoparticles and shows the ability to detect differences in surface functionalisation or composition of nanosized particles. This non-destructive and rapid method is well-suited for industrial particle characterisation and biological applications.
Article
Chemistry, Physical
William H. Hoffmann, Bo Gao, Niall M. C. Mulkerns, Alexander G. Hinton, Simon Hanna, Simon R. Hall, Henkjan Gersen
Summary: This study presents an extension of Nanoparticle Tracking Analysis that allows for the determination of nanorod dimensions and aggregation state. The results are compared with transmission electron microscopy and show the accuracy and reliability of this technique for characterizing nanorods.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
William H. Hoffmann, Niall M. C. Mulkerns, Simon R. Hall, Henkjan Gersen
Summary: Increasing laser power leads to more particles being revealed, but changes in particle residence time due to laser-induced convection can impact size distribution. Using a shutter to inhibit convection currents helps reduce error in size distribution and allows for more robust size characterization of mesoscopic organic clusters. Understanding laser-induced convection is crucial for accurate size determination in two-step nucleation theory.
NANOSCALE ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Baosheng Du, Chengchun Tang, Dan Zhao, Hong Zhang, Dengfeng Yu, Miao Yu, Krishna C. Balram, Henkjan Gersen, Bin Yang, Wenwu Cao, Changzhi Gu, Flemming Besenbacher, Junjie Li, Ye Sun
