期刊
STEM CELLS INTERNATIONAL
卷 2016, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2016/4726267
关键词
-
资金
- Russian Science Foundation [14-15-00693]
- Presidium of Russian Academy of Sciences
- Russian Science Foundation [14-15-00693] Funding Source: Russian Science Foundation
Human adipose tissue-stromal derived cells (ASCs) are considered a perspective tool for regenerative medicine. Depending on the application mode ASC/allogeneic immune cell interaction can occur in the systemic circulation under plenty high concentrations of O-2 and in target tissues at lower O-2 levels. Here we examined the effects of allogeneic PHA-stimulated peripheral blood mononuclear cells (PBMCs) on ASCs under ambient (20%) oxygen and physiological hypoxia (5% O-2). As revealed with microarray analysis ASCs under 20% O-2 were more affected by activated PBMCs, which was manifested in differential expression of more than 300 genes, whereas under 5% O-2 only 140 genes were changed. Altered gene pattern was only partly overlapped at different O-2 conditions. Under O-2 ASCs retained their proliferative and differentiative capacities, mesenchymal phenotype, and intracellular organelle' state. ASCs were proinflammatory activated on transcription level that was confirmed by their ability to suppress activation and proliferation of mitogen-stimulated PBMCs. ASC/PBMCs interaction resulted in anti-inflammatory shift of paracrine mediators in conditioning medium with significant increase of immunosuppressive LIF level. Our data indicated that under both ambient and tissue-related O-2 ASCs possessed immunosuppressive potential and maintained functional activity. Under physiological hypoxia ASCs were less susceptible to priming by allogeneic mitogen-activated PBMCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据