期刊
JOURNAL OF THE AMERICAN HEART ASSOCIATION
卷 5, 期 4, 页码 -出版社
WILEY
DOI: 10.1161/JAHA.115.002288
关键词
arteriogenesis; blood outgrowth endothelial cells; cell transplantation; ischemic heart disease; therapeutic neovascularization
资金
- Research Foundation Flanders (FWO)
- KULeuven Research Grant [PF\10\014]
- ISCIII [PI13/02144, CP09/00333]
- BOF-KU Leuven GOA project [3M120209]
- Interuniversity Attraction Poles (IUAP) [IUAP/P7/07]
Background-Blood outgrowth endothelial cells (BOECs) mediate therapeutic neovascularization in experimental models, but outgrowth characteristics and functionality of BOECs from patients with ischemic cardiomyopathy (ICMP) are unknown. We compared outgrowth efficiency and in vitro and in vivo functionality of BOECs derived from ICMP with BOECs from age-matched (ACON) and healthy young (CON) controls. Methods and Results-We isolated 3.6 +/- 0.6 BOEC colonies/100x10(6) mononuclear cells (MNCs) from 60-mL blood samples of ICMP patients (n=45; age: 66 +/- 1 years; LVEF: 31 +/- 2%) versus 3.5 +/- 0.9 colonies/100x10(6) MNCs in ACON (n=32; age: 60 +/- 1 years) and 2.6 +/- 0.4 colonies/100x10(6) MNCs in CON (n=55; age: 34 +/- 1 years), P=0.29. Endothelial lineage (VEGFR2(+)/CD31(+)/CD146(+)) and progenitor (CD34(+)/CD133(-)) marker expression was comparable in ICMP and CON. Growth kinetics were similar between groups (P=0.38) and not affected by left ventricular systolic dysfunction, maladaptive remodeling, or presence of cardiovascular risk factors in ICMP patients. In vitro neovascularization potential, assessed by network remodeling on Matrigel and three-dimensional spheroid sprouting, did not differ in ICMP from (A) CON. Secretome analysis showed a marked proangiogenic profile, with highest release of angiopoietin-2 (1.4 +/- 0.3x10(5) pg/10(6) ICMP-BOECs) and placental growth factor (5.8 +/- 1.5x10(3) pg/10(6) ICMP BOECs), independent of age or ischemic disease. Senescence-associated beta-galactosidase staining showed comparable senescence in BOECs from ICMP (5.8 +/- 2.1%; n=17), ACON (3.9 +/- 1.1%; n=7), and CON (9.0 +/- 2.8%; n=13), P=0.19. High-resolution microcomputed tomography analysis in the ischemic hindlimb of nude mice confirmed increased arteriogenesis in the thigh region after intramuscular injections of BOECs from ICMP (P=0.025; n=8) and CON (P=0.048; n=5) over vehicle control (n=8), both to a similar extent (P=0.831). Conclusions-BOECs can be successfully culture-expanded from patients with ICMP. In contrast to impaired functionality of ICMP-derived bone marrow MNCs, BOECs retain a robust proangiogenic profile, both in vitro and in vivo, with therapeutic potential for targeting ischemic disease.
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