Article
Plant Sciences
Caihong Jia, Zhuo Wang, Jingyi Wang, Hongxia Miao, Jianbin Zhang, Biyu Xu, Juhua Liu, Zhiqiang Jin, Jihong Liu
Summary: This study identified members of the WRKY transcription factor family in the Musa acuminata genome and categorized them into three subfamilies. Expression analysis showed that most WRKY transcription factors exhibited diverse expression patterns during fruit development, ripening, and in response to various stressors.
Article
Biochemistry & Molecular Biology
Zuowei Xu, Yutong Liu, Huiting Fang, Yanqiong Wen, Ying Wang, Jianxia Zhang, Changcao Peng, Jianmei Long
Summary: In this study, 85 WRKY genes were identified in the genome of N. cadamba, and they were divided into three groups based on their phylogenetic features. The NcWRKY genes were unevenly distributed on 22 chromosomes, and there were two pairs of segmentally duplicated events. The transcript levels of NcWRKY showed distinct expression patterns in various tissues and at different stages of vascular development, and they responded to hormone and abiotic stress treatments. Some NcWRKY genes were found to regulate the biosynthesis of cadambine in response to Methyl jasmonate treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Caijuan Zhang, Wentao Wang, Donghao Wang, Suying Hu, Qian Zhang, Zhezhi Wang, Langjun Cui
Summary: This study systematically analyzed the WRKY gene family in Scutellaria baicalensis, identifying 77 members classified into three subfamilies. The research provided insights into the spatial expression profiles and stress-related expression patterns of WRKYs in different tissues. Additionally, subcellular localization analysis revealed the nuclear localization of certain WRKY proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
Yaqiong Wu, Shanshan Zhang, Xin Huang, Lianfei Lyu, Weilin Li, Wenlong Wu
Summary: This study identified and analyzed the WRKY gene family in black raspberry and classified them into three groups through phylogenetic analysis. The conserved motif analysis revealed three core conserved domains in this gene family. Gene expression analysis showed that 12 WRKY genes were highly expressed in black fruits and leaves. Furthermore, the expression difference at different treatment times indicated that the response of most WRKY transcription factors to NaCl and ABA treatment in black raspberry is temporal.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Ziyun Wan, Xueqin Li, Hefeng Cheng, Jing Zhang, Yujia Chen, Yanxia Xu, Songheng Jin
Summary: This study identified 57 RsWRKYs distributed on 13 chromosomes, divided into three groups based on gene structure and phylogenetic relationships. The highly conserved domain WRKYGQK with three variants was observed in RsWRKYs. The expression patterns of most RsWRKY genes responded to multiple phytohormones and abiotic stressors, providing a foundation for breeding stress-tolerant varieties of Rhododendron.
Article
Genetics & Heredity
Tingting Zhang, Ying Xu, Yadan Ding, Wengang Yu, Jian Wang, Hanggui Lai, Yang Zhou
Summary: This study identified WRKY genes in D. catenatum that may play a role in abiotic stress response and found their tissue-specificity and different expression patterns.
FRONTIERS IN GENETICS
(2022)
Article
Agronomy
Xiaoyang Zhu, Ke Ma, Yulin Yao, Zunyang Song, Yiwei Zhou, Zhengwei Si, Hongyu Lu, Weixin Chen, Xueping Li
Summary: BTH delays fruit ripening and maintains fruit firmness by inhibiting enzymes involved in softening. It also delays and reduces the occurrence of anthracnose caused by Colletotrichum musae.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Plant Sciences
Yingying Yang, Chaojie Wu, Wei Shan, Wei Wei, Yating Zhao, Jianfei Kuang, Jianye Chen, Yueming Jiang, Wangjin Lu
Summary: In this study, the researchers found that MAPK/MPK cascades play important roles in plant growth, development, immune response, and stress tolerance. They demonstrated that MaMPK14 and MaMYB4 negatively regulate banana fruit ripening. MaMYB4 inhibits the expression of genes associated with ethylene biosynthesis and fruit softening, while MaMPK14 phosphorylates MaMYB4 at Ser160 to enhance its transcriptional inhibition, binding strength, protein stability, and repression of fruit ripening.
HORTICULTURE RESEARCH
(2023)
Article
Agricultural Engineering
Yingyu Zhang, Wenwu Zhang, Muhammad Aamir Manzoor, Irfan Ali Sabir, Pengfei Zhang, Yunpeng Cao, Cheng Song
Summary: This study identified 64 WRKY genes in the genome of Dendrobium huoshanense and revealed their involvement in responding to both biotic and abiotic stresses. The subcellular localization analysis showed that most of the WRKY genes were located in the nucleus. The evolutionary analysis provided insights into the origin and duplication events of the WRKY genes in Dendrobium species, and identified DhWRKY42 as a key regulator involved in the regulation of jasmonic-acid-responsive genes.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Yong-Lu Wei, Jian-Peng Jin, Di Liang, Jie Gao, Jie Li, Qi Xie, Chu-Qiao Lu, Feng-Xi Yang, Gen-Fa Zhu
Summary: We conducted a genome-wide investigation and functional analysis of WRKY transcription factors in Cymbidium sinense. We found that some of the genes in this family were strongly responsive to plant hormones and enhanced plant tolerance to abiotic stress within the ABA-dependent pathway.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ping Du, Qinglian Wu, Yihua Liu, Xue Cao, Wenyi Yi, Tikun Jiao, Mengqi Hu, Ying Huang
Summary: WRKY transcription factors play critical roles in plant growth, development, and stress responses. Through genome analysis, 76 WRKY TFs were identified in asparagus lettuce, and their characteristics, phylogenetic relationships, distribution, and expression patterns were analyzed.
Article
Cell Biology
Chaojie Wu, Wei Deng, Wei Shan, Xuncheng Liu, Lisha Zhu, Danling Cai, Wei Wei, Yingying Yang, Jianye Chen, Wangjin Lu, Jianfei Kuang
Summary: In this study, the regulatory pathway of the MaMPK6-3/11-4-MabZIP21 module in controlling banana fruit ripening was characterized. MaMKK1 was found to interact with and phosphorylate MaMPK6-3 and MaMPK11-4, influencing their phosphorylation and activity, and subsequently affecting fruit ripening. MabZIP21 was also found to activate transcription of MaMPK11-4 and MaMKK1, forming a regulatory feedback loop.
Article
Plant Sciences
Weihuang Wu, Sheng Zhu, Lin Xu, Liming Zhu, Dandan Wang, Yang Liu, Siqin Liu, Zhaodong Hao, Ye Lu, Liming Yang, Jisen Shi, Jinhui Chen
Summary: This study conducted a genome-wide analysis of the WRKY gene family in Liriodendron chinense and found that several WRKY genes respond to cold, heat, or drought stress. The LchiWRKY gene family was divided into three phylogenetic groups, and group II was further divided into five subgroups. The WRKY genes were evenly distributed across 15 chromosomes and showed evidence of tandem duplication. The genes mainly underwent purifying selection and were enriched in certain biological processes and molecular functions. Promoter cis-element analysis revealed their role in environmental adaptability. The study provides important insights into the abiotic stress response of L. chinense and can inform afforestation efforts in China.
Article
Biochemistry & Molecular Biology
Maolan Yue, Leiyu Jiang, Nating Zhang, Lianxi Zhang, Yongqiang Liu, Yan Wang, Mengyao Li, Yuanxiu Lin, Yunting Zhang, Yong Zhang, Ya Luo, Xiaorong Wang, Qing Chen, Haoru Tang
Summary: FaWRKY71 plays a significant role in fruit ripening in octoploid strawberry by regulating gene expression, anthocyanin accumulation, and texture softening. This study provides new insights into the regulatory mechanisms of fruit ripening in strawberry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Junjun Zheng, Ziling Zhang, Tao Tong, Yunxia Fang, Xian Zhang, Chunyu Niu, Jia Li, Yuhuan Wu, Dawei Xue, Xiaoqin Zhang
Summary: The WRKY gene family, identified from the barley genome, plays a key role in plant growth and development, secondary metabolite synthesis, and stress responses. Through bioinformatics, 86 WRKY proteins were classified into three groups and seven subgroups, exhibiting differences in structure and phylogenetic relationships. The analysis of gene and protein structure, tissue expression patterns, and cis-elements in the promoter region provided insights into the function of WRKY genes in abiotic stress responses.
Article
Biochemistry & Molecular Biology
Debashrito Deb, Srijita Basak, Tamalika Kar, Utkarsh Narsaria, Filippo Castiglione, Abhirup Paul, Ashutosh Pandey, Anurag P. Srivastava
Summary: This study developed a multi-epitope vaccine using an immunoinformatics approach to combat CHPV, which is more efficient, precise, and less allergenic compared to traditional vaccine design methods.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Plant Sciences
Alok Sharma, Himanshu Sharma, Ruchika Rajput, Ashutosh Pandey, Santosh Kumar Upadhyay
Summary: A series of stress-responsive TLP genes were identified in the genomes of wheat and other cereal crops, and their roles in plant development and stress response were revealed through evolutionary and expression analyses, providing important references for future crop improvement programs.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Priyanka Kumari, Loitongbam Lorinda Devi, Amresh Kumar, Ashutosh Pandey, Subodh Kumar Sinha, Amar Pal Singh
Summary: Nitrogen uptake and assimilation are crucial for plant growth and productivity. This study evaluated eleven rice genotypes under sufficient and low nitrogen conditions, and identified a genotype associated with enhanced nitrogen levels and assimilation. The findings provide important insights for breeding nitrogen use-efficient rice varieties.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Jogindra Naik, Prashant Misra, Prabodh Kumar Trivedi, Ashutosh Pandey
Summary: Flavonoids are structurally diverse compounds that play important roles in plants and have health benefits for humans. Various factors such as light, temperature, nutrients, and hormones modulate their biosynthesis. Transcriptional regulators, especially MYB and bHLH family transcription factors, directly regulate the genes involved in flavonoid biosynthesis. Recent studies have also found that stress and hormone signaling pathways regulate some of these factors.
Article
Plant Sciences
Himanshu Sharma, Alok Sharma, Ruchika Rajput, Sukhjeet Sidhu, Harpal Dhillon, Praveen Chandra Verma, Ashutosh Pandey, Santosh Kumar Upadhyay
Summary: In this study, 29 BOR genes were identified and analyzed for their conserved nature. The role of duplication events and purifying selection in the expansion of the BOR family in wheat was revealed. The study also showed the expression and co-expression patterns of BOR genes under biotic and abiotic stress conditions, as well as their involvement in B stress management.
Article
Genetics & Heredity
Deepika Lakhwani, Yogeshwar Vikarm Dhar, Shikha Singh, Ashutosh Pandey, Prabodh Kumar Trivedi, Mehar Hasan Asif
Summary: The MADS box gene family plays an important role in the growth and development of eukaryotes, particularly in floral development and fruit ripening in plants. Through studying the divergence of the MADS box gene family between Musa balbisiana and Musa acuminata, significant differences are found at the genetic level, which may be correlated with their distinct floral and fruit traits.
Article
Plant Sciences
Umar Gani, Abhishek Kumar Nautiyal, Maridul Kundan, Biswaranjan Rout, Ashutosh Pandey, Prashant Misra
Summary: The NtMATE21 and NtMATE22 genes in tobacco play important roles in flavonol transport, plant growth and development. The expression levels of these two genes are higher in trichomes and their corresponding transporters are localized to the plasma membrane.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Ritu Singh, Ashutosh Pandey, Praveen Kumar Verma
Summary: In this study, the GARP gene family in three major leguminous plants was explored from genomic, molecular, and evolutionary perspectives. The results showed the conservation of the GARP gene family in plants and the expansion through genome duplication in legumes. The GARP genes were found to play a major role in signaling, growth, development, and stress processes. The study also provided stress-responsive genes and laid the foundation for prospective functional studies of GARPs in legumes.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Ruchika Rajput, Jogindra Naik, Ralf Stracke, Ashutosh Pandey
Summary: This study characterized the transcriptional regulatory network of proanthocyanidin biosynthesis in banana. The researchers identified two activators and four repressors that fine tune the biosynthesis of proanthocyanidins. The activators interact with other proteins to transactivate the genes involved in proanthocyanidin biosynthesis, while the repressors form complexes with these proteins to repress proanthocyanidin accumulation. Moreover, the study revealed a competitive relationship between the activators and the repressors, providing insights into the agile regulation of proanthocyanidin biosynthesis.
Article
Plant Sciences
Lalita Pal, Vikas Dwivedi, Santosh Kumar Gupta, Samiksha Saxena, Ashutosh Pandey, Debasis Chattopadhyay
Summary: In this study, we identified the key genes CaMATE1 and CabHLH that regulate flower and seed coat colour in chickpea. Silencing these genes resulted in reduced expression and content of anthocyanin and proanthocyanidin (PA) biosynthetic genes, producing flowers and seeds with characteristics similar to kabuli chickpeas.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Ruchika Rajput, Jogindra Naik, Prashant Misra, Prabodh Kumar Trivedi, Ashutosh Pandey
Summary: Climate change affects crop productivity and developing stress-tolerant and climate-flexible crops is crucial. Genetic engineering is a method used to improve crop traits, but manipulating multiple genes simultaneously is technically challenging. In the last two decades, new methods of gene stacking have been developed in crop biotechnology to overcome this challenge.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Ruchika Rajput, Shivi Tyagi, Jogindra Naik, Boas Pucker, Ralf Stracke, Ashutosh Pandey
Summary: This study identified the major components of the proanthocyanidin regulatory network in chickpea and suggested CaPARs as promising targets for genetic engineering to improve agronomic traits. The researchers discovered 119 typical CaMYB encoding genes and two CaR2R3-MYB transcription factors, named CaPAR1 and CaPAR2, which were found to interact with other key regulators in proanthocyanidin biosynthesis. Transgenic expression of CaPARs in a mutant plant also led to complementation of the transparent testa phenotype.
Article
Plant Sciences
Samiksha Saxena, Lalita Pal, Jogindra Naik, Yeshveer Singh, Praveen Kumar Verma, Debasis Chattopadhyay, Ashutosh Pandey
Summary: Flavonoids play important roles in plants as pigments and defense compounds. This study investigates the transcriptional regulation of flavonoid biosynthesis by the R2R3-MYB transcription factor CaMYB39 in chickpea. Overexpression of CaMYB39 affects the phenylpropanoid pathway and leads to increased levels of isoflavonoids. CaMYB39 activates the transcription of early flavonoid biosynthetic genes, particularly FLAVONOL SYNTHASE2, explaining the preferential accumulation of kaempferol derivatives in chickpea. Additionally, CaMYB39 overexpression enhances trichome density, induces defense gene expression, and reduces symptoms of the fungal pathogen Ascochyta rabiei, providing resistance against Ascochyta blight.
Article
Plant Sciences
Jogindra Naik, Shivi Tyagi, Ruchika Rajput, Pawan Kumar, Boas Pucker, Naveen C. Bisht, Prashant Misra, Ralf Stracke, Ashutosh Pandey
Summary: Flavonols modulate various biological and metabolic pathways, affecting camalexin and aliphatic glucosinolate synthesis, and exhibit multiple biological activities in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Agronomy
Gaurav Pal, Samiksha Saxena, Kanchan Kumar, Anand Verma, Deepak Kumar, Pooja Shukla, Ashutosh Pandey, Satish K. Verma
Summary: This study explores the potential of bacterial lipopeptides in eliciting defense responses in maize seedlings against Fusarium infection. The results showed that lipopeptide treatment enhanced protection against the fungus and improved the growth parameters measured. Gene expression analysis and metabolite analysis revealed the upregulation of defense-related genes and accumulation of antifungal polyphenols.
