Article
Horticulture
Jiawei Zou, Shiqi Gao, Bo Zhang, Wenjie Ge, Jing Zhang, Ruiqin Ji
Summary: Clubroot disease caused by P. brassicae has significantly affected the production of Brassica rapa crops. The mechanism of interaction between P. brassicae and Brassica rapa is not clear. In this study, a differentially expressed protein, BrCAP, was identified and found to play a potential role in the resistance progress of Chinese cabbage to P. brassicae.
Article
Genetics & Heredity
Qi Xie, Xiaochun Wei, Yumei Liu, Fengqing Han, Zhansheng Li
Summary: In this study, we successfully bred highly resistant Brassicaceae materials against clubroot disease and identified several resistant genes. Additionally, we observed the differences in infection process between resistant and susceptible broccoli cultivars.
Article
Plant Sciences
Wenjie Ge, Mingcan Lv, Hui Feng, Xinlei Wang, Bo Zhang, Ken Li, Jing Zhang, Jiawei Zou, Ruiqin Ji
Summary: In this experiment, the differences in BrRPP1 between resistant and susceptible Chinese cabbage were analyzed, and the gene function was verified in Arabidopsis mutants. It was found that the high expression of BrRPP1 in resistant varieties enhanced their resistance to the pathogen. The interaction proteins of BrRPP1 are mainly related to photosynthesis, cell wall modification, jasmonic acid signal transduction, and programmed cell death.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yanyan Wang, Xianyu Xiang, Fan Huang, Wenlin Yu, Xueqing Zhou, Baojun Li, Yunyun Zhang, Peng Chen, Chunyu Zhang
Summary: In this study, we identified two loci CRA8.1a and CRA8.1b of the clubroot-resistant gene CRA8.1 in cabbage material DingWen (DW). We also identified two genes encoding TIR-NBS-LRR proteins and one gene encoding an RLP protein as the most likely candidates for the peculiar resistance in DW.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yuxiang Yuan, Liuyue Qin, Henan Su, Shuangjuan Yang, Xiaochun Wei, Zhiyong Wang, Yanyan Zhao, Lin Li, Honglei Liu, Baoming Tian, Xiaowei Zhang
Summary: Clubroot, caused by Plasmodiophora brassicae, is one of the most destructive diseases of Chinese cabbage worldwide, but the resistance mechanisms are still unclear. This study identified genes associated with clubroot resistance, including those related to auxin, PR, oxidative stress, and transcription factors. The results provide valuable information for better understanding the molecular regulatory mechanism of Chinese cabbage clubroot resistance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Heather H. Tso, Leonardo Galindo-Gonzalez, Troy Locke, Stephen E. Strelkov
Summary: This study describes the development of two rapid and sensitive technologies for pathotyping of Plasmodiophora brassicae. Both assays showed high-throughput potential and accuracy, making them promising as SNP-based pathotype identification tools for clubroot diagnostics.
Article
Plant Sciences
Caixia Gan, Chenghuan Yan, Wenxing Pang, Lei Cui, Pengyu Fu, Xiaoqing Yu, Zhengming Qiu, Meiyu Zhu, Zhongyun Piao, Xiaohui Deng
Summary: This study reveals the inheritance and molecular mechanisms of clubroot resistance in radish, identifying a novel locus responsible for resistance and obtaining core breeding materials for resistance breeding using marker-assisted selection technology.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Muhammad Asim Javed, Arne Schwelm, Nazanin Zamani-Noor, Rasha Salih, Marina Silvestre Vano, Jiaxu Wu, Melaine Gonzalez Garcia, Thies Marten Heick, Chaoyu Luo, Priyavashini Prakash, Edel Perez-Lopez
Summary: Plasmodiophora brassicae is a major threat to the rapeseed and brassica vegetable industry, causing clubroot disease in cruciferous plants. The disease is characterized by wilting, stunting, yellowing, and the presence of club-shaped galls in the roots.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Juan Camilo Ochoa, Soham Mukhopadhyay, Tomasz Bieluszewski, Malgorzata Jedryczka, Robert Malinowski, William Truman
Summary: The RPB1 gene plays a critical role in the defense response of Arabidopsis against Plasmodiophora brassicae infection, and its deletion renders resistant accessions susceptible to the pathogen.
Article
Horticulture
Andrea Botero-Ramirez, Fabian Leonardo Padilla-Huertas, Stephen E. Strelkov, Celsa Garcia-Dominguez
Summary: Clubroot is a major disease of cruciferous crops in Colombia, and its distribution and epidemiology in the country are not well understood. This study found that more than half of the surveyed fields were clubroot-infested, with the highest infestation rate in Norte de Santander. Pathogen inoculum density and average temperature were both positively associated with the likelihood of field infestation.
Article
Plant Sciences
Yingzhe Hong, Siqi Wang, Jie Feng, Yue Liang
Summary: The study found that solarization with mulching can effectively reduce the population of Plasmodiophora brassicae in the soil, thereby decreasing the incidence and severity of clubroot.
Article
Plant Sciences
Jiaxu Wu, Edel Perez-Lopez
Summary: Clubroot, caused by Plasmodiophora brassicae, is a devastating disease that affects cruciferous crops worldwide. The use of resistant cultivars is currently the best strategy to control the disease, but new pathotypes are emerging that can overcome resistance. Understanding the genomics of the pathogen and host is crucial for finding a stable solution for growers.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Piotr M. Kopec, Katarzyna Mikolajczyk, Ewa Jajor, Agnieszka Perek, Joanna Nowakowska, Christian Obermeier, Harmeet Singh Chawla, Marek Korbas, Iwona Bartkowiak-Broda, Wojciech M. Karlowski
Summary: Clubroot, caused by Plasmodiophora brassicae infection, is a significant disease affecting cruciferous crops, with limited effective protection measures available. Breeding resistant varieties remains crucial in disease control, despite the presence of multiple described resistance loci that require ongoing updates to avoid resistance breakdowns.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao
Summary: In this study, circRNA-seq was performed to investigate the role of circRNAs in clubroot disease resistance in Brassica rapa. Differentially expressed circRNAs were identified and a circRNA-miRNA-mRNA network was constructed. One upregulated circRNA was found to suppress the expression of a miRNA and upregulate a gene, possibly leading to plant resistance. These findings provide new insights into clubroot resistance mechanisms and lay the foundation for further studies on complex gene regulation networks in B. rapa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Dinesh Adhikary, Devang Mehta, R. Glen Uhrig, Habibur Rahman, Nat N. V. Kav
Summary: This study used proteomics to investigate the interaction between P. brassicae pathotype 3 and canola, revealing the involvement of proteins related to calcium signaling, reactive oxygen species (ROS) biochemistry, thaumatin, and other factors. In addition, several proteins orthologous to known clubroot resistance (CR) genes were identified. This research improves our understanding of the protein-level mechanisms involved in canola's response to P. brassicae and provides insights for developing CR cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kang Zhang, Yinqing Yang, Jian Wu, Jianli Liang, Shumin Chen, Lei Zhang, Honghao Lv, Xiaona Yin, Xin Zhang, Yiyue Zhang, Lingkui Zhang, Yangyong Zhang, Michael Freeling, Xiaowu Wang, Feng Cheng
Summary: Leaf heading is an important horticultural trait in vegetables. This study identifies the transcriptional regulation mechanism and developmental switch involved in the transition to leaf heading in Chinese cabbage and cabbage. The launch of the transition stage is closely associated with ambient temperature, and the ethylene pathway is hypothesized to have been targeted for selection during the domestication of Chinese cabbage to form the heading trait. These findings provide new insights for future studies of leafy head crops.
Review
Biochemistry & Molecular Biology
Jialei Ji, Limei Yang, Zhiyuan Fang, Yangyong Zhang, Mu Zhuang, Honghao Lv, Yong Wang
Summary: This review focuses on the advances of the plant SWEETs, including their discovery, protein structure characteristics, evolution, physiological functions, and applications in plant breeding. It provides more in-depth and comprehensive information to elucidate the molecular basis of SWEETs' function in plants.
Article
Plant Sciences
Zhansheng Li, Lixiao Song, Yumei Liu, Fengqing Han, Wei Liu
Summary: In this study, comparisons of morphological, electrophysiological and transcriptomic profiles in isogenic Ogura-CMS, DGMS and maintainer broccoli lines revealed differences in agronomic traits, growth periods and gene expression patterns, providing insights into the mechanism of male sterility in Brassica crops.
Article
Chemistry, Multidisciplinary
Qi Xie, Yongyu Zhao, Yumei Liu, Fengqing Han, Wei Liu, Zhansheng Li
Summary: This study utilized SSR marker technology to perform DNA fingerprinting of 10 broccoli varieties, and successfully identified markers with good polymorphism to establish the fingerprinting of these varieties. This provides a scientific basis for the identification of multiple clubroot resistance genes in broccoli.
APPLIED SCIENCES-BASEL
(2022)
Article
Genetics & Heredity
Qi Xie, Xiaochun Wei, Yumei Liu, Fengqing Han, Zhansheng Li
Summary: In this study, we successfully bred highly resistant Brassicaceae materials against clubroot disease and identified several resistant genes. Additionally, we observed the differences in infection process between resistant and susceptible broccoli cultivars.
Article
Chemistry, Applied
Zhansheng Li, Guangmin Liu, Hongju He, Yumei Liu, Fengqing Han, Wei Liu
Summary: The effects of a nanocarbon solution on nutrient content, glucosinolate metabolism, and glucoraphanin pathway in broccoli were studied. The nanocarbon solution positively influenced total protein yield and glucoraphanin content, but had no effect on soluble sugars, vitamin C, and dry matter production. The 18.75 L.ha(-1) nanocarbon solution significantly upregulated the expression levels of specific genes responsible for glucoraphanin and glucoerucin accumulation. This study provides insights into using nanotechnological approaches to develop efficient and environmentally friendly nanocarbon solutions for crops.
Article
Biochemistry & Molecular Biology
Li Chen, Wenjing Ren, Bin Zhang, Huiling Guo, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Xilin Hou, Yangyong Zhang
Summary: In this study on cabbage, the cytology and transcriptome analysis were performed to investigate the microspore development process and gene expression changes in Ogura cytoplasmic male sterility (CMS) lines. The results showed that early degradation of tapetal cells in Ogura CMS caused pollen abortion. Transcriptome analysis revealed the dysregulated expression of genes related to energy metabolism in Ogura CMS, leading to decreased ATP content and excessive ROS accumulation in anthers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Peng Wang, Wenxue Cao, Limei Yang, Yangyong Zhang, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Shanhan Cheng, Jialei Ji
Summary: The synthesis of glucosinolates (GSLs) in cabbage was studied by analyzing the cabbage genome and identifying 193 GSLs biosynthetic genes (GBGs). It was found that the expression levels of these GBGs were significantly altered by different exogenous hormones. This study provides a foundation for regulating GSLs synthesis in cabbage through gene editing and overexpression.
Article
Plant Sciences
Kaiwen Yuan, Xinyu Zhao, Wenru Sun, Limei Yang, Yangyong Zhang, Yong Wang, Jialei Ji, Fengqing Han, Zhiyuan Fang, Honghao Lv
Summary: This study identified the gene BoNA1, which is responsible for the absence of anthocyanins in curly kale. The function of BoNA1 was confirmed through gene editing technology, improving our understanding of the regulation mechanism of anthocyanin accumulation in Brassica oleracea subspecies.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Jiao Wang, Bin Zhang, Huiling Guo, Li Chen, Fengqing Han, Chao Yan, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Yangyong Zhang
Summary: In this study, the molecular regulatory mechanism underlying flowering time regulation in cabbage was investigated using transcriptome analysis. Two candidate genes, BoSEP2-1 and BoSEP2-2, were identified. These findings provide new insights into the molecular mechanisms underlying flowering time regulation in cabbage.
Article
Microbiology
Yuankang Wu, Bin Zhang, Shaobo Liu, Zhiwei Zhao, Wenjing Ren, Li Chen, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Fengqing Han, Yangyong Zhang
Summary: This study presents the first whole-genome sequence of Hyaloperonospora parasitica isolate BJ2020, a pathogen that causes downy mildew in cabbage. The genome contains 4631 contigs and 9991 protein-coding genes, with a size of 37.10 Mb. Comparative analysis revealed the close phylogenetic affinity between H. parasitica and H. brassicae, providing important insights into the pathogenic mechanisms of H. parasitica BJ2020.
Article
Agronomy
Longxiang Yan, Zhiyuan Fang, Na Zhang, Limei Yang, Yangyong Zhang, Mu Zhuang, Honghao Lv, Jialei Ji, Yong Wang
Summary: This study examined 10 BoGGPPS genes from B. oleracea and found that they are mainly expressed in chloroplasts and plastids. Similar BoGGPPS genes have similar structure and motifs. Distribution, collinearity, and Ka/Ks analysis revealed multiple duplications in the BoGGPPS gene family. Cis-acting element analysis suggests that cabbage BoGGPPS genes may participate in light and hormone responses. Three-dimensional structure analysis shows the abundance of α-helices and random coils among BoGGPPS members, indicating their important functions. The findings provide a basis for further exploration and functional analysis of BoGGPPS genes and their relationship with terpenoids in chlorophyll synthesis in B. oleracea.
Article
Horticulture
Dan Wu, Miao Ni, Xin Lei, Liping Zhang, Wei Zhang, Huangying Shu, Zhiwei Wang, Jie Zhu, Shanhan Cheng, Pingwu Liu, Honghao Lv, Limei Yang
Summary: This study analyzed the genome-wide CCR gene family in pepper and identified 38 CCR genes, and characterized the enzymic kinetic parameters and regulatory function of two of them. The study found that CCRs have an impact on capsaicin content, with CCR2 playing a more important role in regulating pungency formation.
Article
Plant Sciences
Mingzhao Zhu, Yong Wang, Shujin Lu, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Zhiyuan Fang, Xilin Hou
Summary: In this study, a total of 36 CKX genes were identified in cabbage using a genome-wide search method. Phylogenetic analysis classified these genes into three groups. These genes were unevenly distributed across nine chromosomes in B. oleracea, with 15 genes not containing any introns. The expression patterns of these genes in various tissues and organs were shown through a heatmap. Three genes were found to be relatively highly expressed in all investigated tissues under normal conditions, while three other genes showed significant down-regulation and up-regulation in susceptible and resistant materials after inoculation, indicating their potential roles in resistance to clubroot disease.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Plant Sciences
Mingzhao Zhu, Limei Yang, Yangyong Zhang, Mu Zhuang, Jialei Ji, Xilin Hou, Zhansheng Li, Fengqing Han, Zhiyuan Fang, Honghao Lv, Yong Wang
Summary: In this study, clubroot resistance (CR) genes from Brassica rapa were introduced into the cabbage genome, making it resistant to clubroot disease. The mechanism of gene introgression was explored through cytoplasmic replacement and distant hybridization, revealing homoeologous exchange as the basis for the introgression of CR resistance. The successful introduction of CR into the cabbage genome provides valuable insights for creating introgression lines in other species of interest.
HORTICULTURE RESEARCH
(2022)
