Article
Virology
Marta Kuzminska-Bajor, Paulina Sliwka, Maciej Ugorski, Pawel Korzeniowski, Aneta Skaradzinska, Maciej Kuczkowski, Magdalena Narajaczyk, Alina Wieliczko, Rafal Kolenda
Summary: Five novel lytic Salmonella phages, UPWr_S1-5, were isolated and characterized in this study. They exhibit efficient infection of various Salmonella serovars and show potential in combating Salmonella infections, making them promising tools for biological control of salmonellosis.
Article
Biochemistry & Molecular Biology
Kristen N. LeGault, Zachary K. Barth, Peter DePaola, Kimberley D. Seed
Summary: This study reveals an unknown mechanism where a nuclease cleaves the viral genome to prevent bacterial virus infection in Vibrio cholerae. It is of great interest for researchers studying phage-host interactions and microbial genetics.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Food Science & Technology
Kirsten Wessels, Diane Rip, Pieter Gouws
Summary: Controlling Salmonella in chicken processing plants is a global challenge, with current chemical antimicrobials losing effectiveness due to rising resistance. Bacteriophages are gaining appeal as an alternative method, offering host specificity and potential for successful implementation in large-scale operations.
Article
Agriculture, Dairy & Animal Science
Matias Aguilera, Eduardo Tobar-Calfucoy, Victoria Rojas-Martinez, Rodrigo Norambuena, Maria Jesus Serrano, Onix Cifuentes, Maria Sofia Zamudio, Daniel San Martin, Pabla Lara, Andrea Sabag, Marcela Zabner, Daniel Tichy, Pamela Camejo, Luis Leon, Michael Pino, Soledad Ulloa, Felipe Rojas, Christian Pieringer, Cecilia Muster, Daniel Castillo, Nicolas Ferreira, Camilo Avendano, Mauro Canaval, Hans Pieringer, Pablo Cifuentes, Nicolas Cifuentes Munoz
Summary: In this study, we isolated and characterized 47 bacteriophages that showed variable degrees of lytic activity against 258 Salmonella isolates from a commercial broiler company in Brazil. Three of these bacteriophages were selected to assemble a cocktail, which was found to be highly effective against multiple serovars of Salmonella in vitro, including under conditions that resemble the chicken gut. The cocktail also showed limited or no cross-resistance, highlighting its potential for in vivo application in commercial broiler farms.
Article
Microbiology
Jialiang Xu, Jia Li, Yi Yan, Pengjun Han, Yigang Tong, Xu Li
Summary: A lytic phage SW16-7 was isolated from medical sewage, showing high infectivity against Salmonella enterica serovar Weltevreden and other strains. In vitro inhibition assays demonstrated its effective antibacterial effect for up to 12 h. The phage's genome lacks virulence factors and antibiotic resistance genes, suggesting its potential as a biocontrol agent against S. Weltevreden.
Article
Agriculture, Dairy & Animal Science
E. A. Kimminau, T. P. Karnezos, K. N. Russo, J. A. Baxter, R. D. Berghaus, M. Jones, C. L. Hofacre
Summary: The study found that dietary bacteriophages do not interfere with the colonization or protection provided by the live Salmonella vaccine. There were no significant differences in Salmonella prevalence in cloacal swabs and cecal cultures among all treatment groups, but T2 and T3 had lower mortality and feed conversion ratio compared to T1.
Article
Biotechnology & Applied Microbiology
Yanhua Jiang, Dongqin Xu, Lianzhu Wang, Meng Qu, Fengling Li, Zhijun Tan, Lin Yao
Summary: The recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained in this study, and EDTA was found to enhance its bactericidal activity. LysSP1 showed strong bactericidal activity against Salmonella Typhimurium, exhibited good stability, and demonstrated broad-spectrum bactericidal ability.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: In this study, a lytic phage STG2 capable of reducing S. enteritidis and S. typhimurium contaminations on cabbage and various surfaces was characterized. Genomic characterization revealed that phage STG2 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing both planktonic cells and biofilms on different surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Adam J. Hockenberry, Claus O. Wilke
Summary: BACPHLIP is a tool that accurately predicts the lifestyle of bacteriophages by detecting conserved protein domains and using a trained classifier. It achieved an accuracy of 98% on a test set, greatly exceeding previous tools.
Article
Microbiology
Janet Y. Nale, Gurinder K. Vinner, Viviana C. Lopez, Anisha M. Thanki, Preeda Phothaworn, Parameth Thiennimitr, Angela Garcia, Manal AbuOun, Muna F. Anjum, Sunee Korbsrisate, Edouard E. Galyov, Danish J. Malik, Martha R. J. Clokie
Summary: The study found that phages can be used to control Salmonella infection in animals, and specific application methods were investigated. Prophylactic regimen was more effective than remedial regimen, showing promising potential for treating infections in pigs and poultry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Payel Mondal, Bani Mallick, Moumita Dutta, Shanta Dutta
Summary: Salmonella is a common pathogen causing bacterial gastroenteritis in children and the elderly. A lytic bacteriophage, STWB21, was isolated and found to have bactericidal activity against Salmonella typhi and other bacterial species, as well as reducing the bacterial population in biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Rocio Barron-Montenegro, Dacil Rivera, Maria Jesus Serrano, Rodrigo Garcia, Diana M. alvarez, Julio Benavides, Fernanda Arredondo, Francisca P. alvarez, Roberto Bastias, Soledad Ruiz, Christopher Hamilton-West, Eduardo Castro-Nallar, Andrea I. Moreno-Switt
Summary: Salmonella spp. is a worldwide foodborne pathogen, and bacteriophages represent an alternative to antimicrobials for mitigating Salmonella infections. In this study, Salmonella Enteritidis and a lytic phage were coevolved in a culture medium for 21 days. The results showed that both bacteria and phage could survive and develop resistance to each other during the coevolution process. The interactions between Salmonella and lytic phages are crucial for the rational use of phages to control this important foodborne pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Jun Kwon, Sang Guen Kim, Sib Sankar Giri, Hyoun Joong Kim, Sang Wha Kim, Jeong Woo Kang, Sung Bin Lee, Won Jun Jung, Cheng Chi, Se Chang Park
Summary: This study successfully isolated and characterized a novel bacteriophage that infects Salmonella, which belongs to a new genus. These findings contribute to our understanding of the phylogeny of bacteriophages and have potential implications for the development of bacteriophage treatments against Salmonella infections.
ARCHIVES OF VIROLOGY
(2022)
Article
Food Science & Technology
Junxin Zhao, Yunzhi Lin, Chen Wang, Mahmoud Zayda, Aye Thida Maung, Tahir Noor Mohammadi, Hoang Minh Duc, Ping Yu, Maomao Ma, Deming Gong, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Zheling Zeng
Summary: In this study, a novel Salmonella phage PS3-1 was identified with high lytic activity against Salmonella Typhimurium. PS3-1 belonged to the class Caudoviricetes with a broad host range, and had relatively short latent period, large burst size, high pH stability and thermal tolerance. Genome sequencing analysis showed that PS3-1 genome consisted of 107,110 bp DNA, without antibiotic resistance and virulence related genes.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Maria Dolores Blanco Fernandez, Jochen Klumpp, Melina E. Barrios, Viviana A. Mbayed
Summary: Listeria bacteriophages, belonging to the genus Pecentumvirus, are promising tools for controlling Listeria monocytogenes in food and surfaces. The study of 21 phages in this genus has provided insight into Pecentumvirus evolution, showing distinct gene gain and loss propensity, recombination events, and two groups of distantly related species formed through diversification.
Article
Engineering, Environmental
David Fernandez-Verdejo, Pilar Cortes, Paqui Blanquez, Ernest Marco-Urrea, Albert Guisasola
Summary: This study demonstrates that using different carbon-based cathodic electrode materials in BES can promote the growth of Dehalogenimonas and the degradation of 1,2-DCP, with the graphite brush electrode showing better performance.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xu Han, Gerard Boix, Mateusz Balcerzak, Oscar Hernando Moriones, Mary Cano-Sarabia, Pilar Cortes, Neus Bastus, Victor Puntes, Montserrat Llagostera, Inhar Imaz, Daniel Maspoch
Summary: This study reports the development of a metal-organic framework based composite for the controlled release of iodine, which exhibits antibacterial properties without causing drug resistance. The composite was successfully integrated into a polymer to create antibacterial films, which showed effective long-term release of iodine. In vitro tests confirmed the antibacterial activity of these films against gram-positive and gram-negative bacteria.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Miquel Sanchez-Osuna, Pilar Cortes, Mark Lee, Aaron T. Smith, Jordi Barbe, Ivan Erill
Summary: Lesions to DNA can threaten cell survival by compromising chromosome integrity, and the bacterial SOS response is a common transcriptional regulatory mechanism to address DNA damage. A novel family of transcriptional repressor proteins in the Bacteroidetes has been identified, orchestrating the canonical response to DNA damage in this phylum. The discovery sheds light on the origin and plasticity of the transcriptional network involved in the SOS response.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Environmental Sciences
Jesica M. Soder-Walz, Clara Torrento, Camelia Algora, Kenneth Wasmund, Pilar Cortes, Albert Soler, Teresa Vicent, Monica Rosell, Ernest Marco-Urrea
Summary: A novel Dehalobacter strain capable of transforming TCM to dichloromethane was obtained, along with its ability to transform other chlorinated compounds. Analysis of 16S rRNA genes confirmed growth of Dehalobacter with TCM and other chlorinated compounds as electron acceptors, and isotope fractionation during the transformation process was studied. These findings demonstrate a potential tool for differentiating TCM degraders in field studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Miquel Sanchez-Osuna, Jordi Barbe, Ivan Erill
Summary: The emergence of multiple antimicrobial resistant strains is a pressing issue in modern healthcare. Our study analyzes the %GC content of plasmid-borne antimicrobial resistance genes to detect and quantify their dissemination. We find that the dissemination of resistance genes depends on the resistance mechanism and that %GC content analysis is a powerful method to monitor their dissemination using publicly available sequence data.
Article
Infectious Diseases
Maria Lajarin-Reinares, Eloy Pena-Rodriguez, Mariona Canellas-Santos, Elisabet Rosell-Vives, Pilar Cortes, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: Topically applied antimicrobial drugs have several advantages, but the widespread use of antibiotics has led to increasing antimicrobial resistance. Drug repurposing, such as with disulfiram, may provide an attractive alternative for treating multidrug-resistant bacteria in skin infections.
Article
Pharmacology & Pharmacy
Maria Lajarin-Reinares, Iria Naveira-Souto, Mireia Mallandrich, Joaquim Suner-Carbo, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: This study aimed to develop and optimize a mucoadhesive drug delivery system for the local administration of disulfiram. The results showed that these gels had antifungal activity against Candida albicans, Candida parapsilosis, and Nakaseomyces glabratus. The physicochemical properties and release profiles of the gels were also characterized and investigated.
Article
Immunology
Julia Lopez-Perez, Jennifer Otero, Miquel Sanchez-Osuna, Ivan Erill, Pilar Cortes, Montserrat Llagostera
Summary: This study investigates the emergence of Salmonella strains with reduced susceptibility to a cocktail of three phages in different scenarios. The results show that the mechanisms of resistance vary in different environments, but they do not significantly impair the success of Salmonella biocontrol and oral phage therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
David Fernandez-Verdejo, Pilar Cortes, Albert Guisasola, Paqui Blanquez, Ernest Marco-Urrea
Summary: This study investigated the sequential transformation of chloroform to acetate using bioelectrochemical systems (BESs) and syntrophic dechlorinating cultures. The results showed the advantages of combining bacteria to fully detoxify chlorinated compounds in BESs, expanding the application of this technology for treating polluted water bodies.
ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY
(2022)
Article
Immunology
Jordi Corral, Maria Perez-Varela, Miquel Sanchez-Osuna, Pilar Cortes, Jordi Barbe, Jesus Aranda
Summary: Acinetobacter baumannii is a clinically significant pathogen with increasing worldwide importance, especially due to its ability to acquire resistance genes. This bacterium can move through two types of motility: twitching and surface-associated, with strain MAR002 exhibiting both. Mutants with altered motility patterns were identified through spontaneous rifampin-resistant mutations in the rpoB gene. Transcriptomic analysis revealed deregulated genes within each motility phenotype, highlighting the key role of surface-associated motility and limited role of twitching in the pathogenicity of A. baumannii strain MAR002.