Article
Agricultural Engineering
Vinod Kumar, Anu Radha, Varsha Sharma, Nagaraju Nekkala, Saurabh Saran
Summary: The aim of this study was to investigate the use of xylose enriched spent lemongrass hydrolysate as an alternative production medium for the production of clavulanic acid by Streptomyces clavuligerus. Xylose was extracted from spent lemongrass using dilute nitric acid and further purified using ion exchange resin. The optimal conditions for cell growth and clavulanic acid production were determined, resulting in an effective production of 0.59 g/L clavulanic acid.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Paloma Liras, Juan F. Martin
Summary: Transcriptomic, proteomic, and metabolomic studies have enhanced our understanding of the biosynthesis and regulation of clavuligerus, leading to the discovery of new metabolites. The role of specific activators and regulators in gene expression has been elucidated, providing insights for improving industrial applications and clavulanic acid production.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Aslihan Kurt-Kizildogan, Gozde Celik, Eser unsaldi, Servet Ozcan, Serife Ayaz-Guner, Gulay Ozcengiz
Summary: This study deciphers regulatory mechanisms in clavulanic acid biosynthesis and provides insights for enhancing CA yields through analyzing transcriptome and proteome alterations in an industrial overproducer strain.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Luisa F. Patino, Vanessa Aguirre-Hoyos, Laura I. Pinilla, Leon F. Toro, Rigoberto Rios-Estepa
Summary: This study found that the overexpression of the orf21 gene can regulate the production of clavulanic acid in Streptomyces, and is influenced by the nutritional composition of the culture medium.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Petra Pivk Lukancic, Tjasa Drcar, Robert Bruccoleri, Martin Crnugelj, Peter Mrak
Summary: In this study, a new genotype of Streptomyces clavuligerus with the ability to produce teleocidin was discovered. Teleocidin is a highly toxic secondary metabolite. The genome sequence of this genotype provides a foundation for further research and application.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Infectious Diseases
Victor A. Lopez-Agudelo, David Gomez-Rios, Howard Ramirez-Malule
Summary: Clavulanic acid is an irreversible beta-lactamase enzyme inhibitor with weak antibacterial activity, typically combined with antibiotics to treat diseases caused by beta-lactam resistant bacteria. Improvements in large-scale bioprocessing and integration of metabolic engineering strategies with systems biology methods are essential to optimize production and reduce costs associated with clavulanic acid.
Article
Biotechnology & Applied Microbiology
Renata M. M. G. P. Ribeiro, Mateus N. Esperanca, Ana P. A. Sousa, Alvaro Baptista Neto, Marcel O. Cerri
Summary: This study evaluated the dissociated effect of oxygen transfer rate and shear rate on the production of clavulanic acid from Streptomyces clavuligerus. The results showed that increasing oxygen transfer rate and shear rate both positively enhanced clavulanic acid productivity.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemical Research Methods
Tao Feng, Jie Zhao, Yan-Feng Bai, Ju Chu, Yong-Hong Wang, Ying-Ping Zhuang
Summary: The study found that 24 degrees Celsius is the most favorable temperature for the synthesis of clavulanic acid by Streptomyces clavuligerus. In both 50 L and 60 m(3) fermenters, using the 26-24 degrees Celsius temperature strategy can increase CA production and shorten fermentation duration.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
David Gomez-Rios, Howard Ramirez-Malule, Peter Neubauer, Stefan Junne, Rigoberto Rios-Estepa, Silvia Ochoa
Summary: In this study, the low productivity of the beta-lactamase inhibitor clavulanic acid (CA) in wild-type Streptomyces clavuligerus was analyzed, and the key role of the central metabolism in regulating the carbon fluxes of CA biosynthesis was identified. Experimental validation showed that CA production could be increased by implementing a fed-batch operation.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Tao Feng, Jie Zhao, Ju Chu, Yong-hong Wang, Ying-ping Zhuang
Summary: This study successfully increased the production level of clavulanic acid to 946 mg/L through statistical optimization of the fermentation medium, achieving a 47% improvement compared to the original medium.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chang-Hun Shin, Hang Su Cho, Hyung-Jin Won, Ho Jeong Kwon, Chan-Wha Kim, Yeo Joon Yoon
Summary: By iterative random mutagenesis and reporter-guided selection, a new mutant strain of CA-producing Streptomyces clavuligerus derived from the industrial strain OR was developed, resulting in significantly increased CA production through overexpression of the glycerol utilization operon.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Infectious Diseases
Ines Mack, Mike Sharland, Janneke M. Brussee, Sophia Rehm, Katharina Rentsch, Julia Bielicki
Summary: AMC, belonging to the WHO Essential Medicines List for children, needs to be stored in refrigerated conditions for optimal effectiveness. A study on the stability of AMC suspensions found significant degradation of clavulanic acid under inappropriate storage conditions, highlighting the importance of proper storage to ensure efficacy in pediatric patients.
Article
Biochemistry & Molecular Biology
Ravi Saini, Sonali Kumari, Amit Singh, Abha Mishra
Summary: The study evaluated the antioxidant, protein kinase inhibitory (PKIs) potential, and cytotoxicity activity of Streptomyces clavuligerus extract. The results showed that the extract had a strong free radical scavenging capacity, inhibitory potential against cancer cell growth, and superior inhibitory potential against FGFR and PDGFR RTKs compared to FDA-approved drugs.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Howard Ramirez-Malule, Victor A. Lopez-Agudelo, David Gomez-Rios, Silvia Ochoa, Rigoberto Rios-Estepa, Stefan Junne, Peter Neubauer
Summary: The study demonstrated that Streptomyces clavuligerus cultivated in 2D rocking bioreactor in fed-batch operation can produce clavulanic acid at comparable rates to those observed in stirred tank bioreactors. A reduced model of S. clavuligerus metabolism was constructed and used for in silico studies of metabolic scenarios during the cultivations. Constraint-based analysis revealed the interrelations between metabolites in submerged cultures of S. clavuligerus, providing insights into the scenarios of low and high clavulanic acid production.
BIOENGINEERING-BASEL
(2021)
Review
Surgery
Rodrigo Moises de Almeida Leite, Dong Joo Seo, Barbara Gomez-Eslava, Sigma Hossain, Arnaud Lesegretain, Alexandre Venancio de Souza, Camden Phillip Bay, Bruno Zilberstein, Evaldo Marchi, Rogerio Bonassi Machado, Leandro Cardoso Barchi, Rocco Ricciardi
Summary: This meta-analysis suggests that nonoperative management is a safe and effective option for uncomplicated acute appendicitis. However, compared to appendectomy, nonoperative management may result in a longer hospital stay and a higher rate of recurrent appendicitis.
Review
Food Science & Technology
Ting Yi Heung, Jessica Yue Shuen Huong, Wan Yeng Chen, Yi Wen Loh, Kooi Yeong Khaw, Bey-Hing Goh, Yong Sze Ong
Summary: Papaya extracts or their bioactive compounds may serve as a potential broad-spectrum therapy for cancer by targeting multiple characteristics of cancer development.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Health Care Sciences & Services
Tesnime Jebara, Ailsa Power, Anne Boyter, Sabrina A. Jacob, Jane Portlock, Scott Cunningham
Summary: This study explores the views and experiences of stakeholders on interprofessional education (IPE) in practice-based experiential learning (EL), as well as the current barriers and enablers in its practice and future development. The findings highlight the stakeholders' belief in building on current IPE while challenging the ethos and culture of EL. All stakeholders should be involved in the development of curriculum to overcome barriers and enhance enablers.
JOURNAL OF INTERPROFESSIONAL CARE
(2023)
Review
Food Science & Technology
Omar Belmehdia, Naoual El Menyiy, Abdelhakim Bouyahya, Aicha El Baaboua, Nasreddine El Omari, Monica Gallo, Domenico Montesano, Daniele Naviglio, Gokhan Zengin, Nadia Skali Senhaji, Bey Hing Goh, Jamal Abrini
Summary: This review discusses the recent advances in the chemical composition and biological activities of propolis. It highlights the various important bioactive properties of propolis, including antibacterial, antioxidant, and anticancer effects. Data shows that propolis has low toxicity even at high doses, making it a promising source of bioactive compounds for drug development.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Pathology
Kar-Yan Su, Jie-Yi Koh Kok, Yie-Wei Chua, Shearn-Dior Ong, Hooi Leng Ser, Priyia Pusparajah, Pui San Saw, Bey Hing Goh, Wai-Leng Lee
Summary: Extracellular vesicles (EVs) are membrane-derived lipid bilayers released by cells. Bacterial EVs (BEVs) have attracted interest as potential diagnostic markers due to their differential bacterial composition in biofluids between patients and healthy controls. This review outlines the commonly used approach for investigating BEVs in biofluids, including 16S ribosomal RNA gene sequencing, and discusses the potential of BEVs as biomarkers for various diseases.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Article
Virology
Kok Keng Tee, Po Qhuan Chan, Alson Mun-Khin Loh, Sarbhan Singh, Chee How Teo, Thevambiga Iyadorai, Jack Bee Chook, Kim Tien Ng, Yutaka Takebe, Kok Gan Chan, I-Ching Sam, Kenny Voon
Summary: Pteropine orthoreovirus (PRV), a bat-borne virus, is associated with acute respiratory infections (ARI) in humans. Among 632 urban outpatients, 2.2% tested positive for PRV. Symptoms included cough, sore throat, and muscle ache, with minimal fever. Genetic analysis revealed the continuous evolution of PRV through reassortment among human, bat, and non-human primate hosts, emphasizing the importance of monitoring its prevalence and diversity.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aurora Garcia-Berro, Venkat Talla, Roger Vila, Hong Kar Wai, Daria Shipilina, Kok Gan Chan, Naomi E. Pierce, Niclas Backstrom, Gerard Talavera
Summary: This study investigates the heterozygosity and population size dynamics of migratory insects using genomic data. The findings show that migratory species have higher levels of genome-wide heterozygosity than nonmigratory species. The study also reveals that migratory Lepidoptera maintain overall genetic diversity and demographic stability despite the risks associated with migration and local environmental fluctuations.
Review
Biochemistry & Molecular Biology
Bingpeng Lin, Hooi Leng Ser, Lijing Wang, Jiang Li, Kok-Gan Chan, Learn-Han Lee, Loh Teng-Hern Tan
Summary: This review provides a comprehensive overview of the role of MMP12 in various oral diseases, including periodontitis, temporomandibular joint dysfunction, orthodontic tooth movement, and oral squamous cell carcinoma. Studies have shown the association of MMP12 expression with the pathogenesis of these oral diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fatemeh Shahdadi, Maliheh Faryabi, Haroon Khan, Ali Salehi Sardoei, Bahman Fazeli-Nasab, Bey Hing Goh, Khang Wen Goh, Ching Siang Tan
Summary: This study examined the antibacterial effects of Mentha longifolia essential oil and pulegone in edible coatings on the growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli in cheese. The results showed that the addition of these compounds significantly reduced bacterial growth during storage. Increasing the concentration of chitosan, M. longifolia, and pulegone in the coatings further decreased the bacterial population, with pulegone exhibiting a stronger effect. Overall, the findings suggest that M. longifolia essential oil and pulegone, combined with alginate and chitosan coatings, have antibacterial effects against S. aureus, L. monocytogenes, and E. coli in cheese.
Editorial Material
Pharmacology & Pharmacy
Kooi Yeong Khaw, Michael R. R. Whittaker, Sanjaya Kuruppu, Kok Gan Chan, Siew Hua Gan, Bey Hing Goh
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Medicine, Research & Experimental
Nasreddine El Omari, Saad Bakrim, Asaad Khalid, Mohammed Albratty, Ashraf N. Abdalla, Learn-Han Lee, Khang Wen Goh, Long Chiau Ming, Abdelhakim Bouyahya
Summary: Cancer progression is influenced by epigenetic events, with histone modification playing a key role in gene expression. Belinostat, a pan-HDAC inhibitor, has shown effectiveness in the treatment of T-cell lymphoma and solid malignancies. By indirectly promoting acetylated histone accumulation and restoring normal gene expressions, belinostat exhibits potential anti-cancer therapeutic effects through various pathways. Additionally, it has been found to increase p21WAF1, a cyclin-dependent kinase inhibitor involved in cell cycle arrest and apoptosis. Investigative trials have supported belinostat's potential as a valuable anti-cancer drug.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Wei Hsum Yap, Bey Hing Goh
Review
Food Science & Technology
Wei-Shan Ang, Jodi Woan-Fei Law, Vengadesh Letchumanan, Kar Wai Hong, Sunny Hei Wong, Nurul Syakima Ab Mutalib, Kok-Gan Chan, Learn-Han Lee, Loh Teng-Hern Tan
Summary: A new probiotic, Christensenella minuta, was discovered in 2012 from healthy human stool. It is a subdominant commensal bacterium with highly heritable properties and has mutual interactions with other heritable microbiomes. Its relative abundance is positively correlated with the lean host phenotype. Various studies have shown its potential in managing metabolic diseases such as obesity, inflammatory bowel disease, and type 2 diabetes.
Article
Agricultural Engineering
Samiah Hamad Al-Mijalli, Naoufal El Hachlafi, Emad M. Abdallah, Mohamed Jeddi, Hamza Assaggaf, Ahmed Qasem, Sulaiman Mohammed Alnasser, Ammar Attar, Mommen Adnan Naem, Learn-Han Lee, Abdelhakim Bouyahya, Hanae Naceiri Mrabti
Summary: In this study, the chemical constituents and antibacterial mechanism of action of Syzygium aromaticum essential oils extracted from leaves (CEOL) and floral buds (CEOB) were investigated. The results showed that eugenol was the major component in both CEOL and CEOB. The essential oils exhibited potent antibacterial activity against various bacteria, with low minimum inhibitory concentrations and bactericidal nature. They also affected the cell membrane permeability and cell integrity, leading to disruption of bacterial growth and viability. Additionally, CEOB showed stronger antimicrobial effects and anti-biofilm activity, suggesting its potential use as a natural alternative in the food and pharmaceutical industries.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Ying Cui, Kai Song, Zi-Jing Jin, Learn-Han Lee, Chitti Thawai, Ya-Wen He
Summary: This study found that minimum medium favored the synthesis of biocontrol strain Pseudomonas PA1201's antimicrobial secondary metabolite, pyoluteorin (Plt). Fructose, mannitol, and glycerol promoted Plt biosynthesis, with fructose being the most effective carbon source. Fructose acted through activation of the pltLABCDEFG (pltL) operon, while glucose or succinic acid antagonized fructose-dependent synthesis of Plt.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
