Article
Food Science & Technology
Luli Zhou, Guanyu Hou, Hanlin Zhou, Khaled Abouelezz, Yuxiu Ye, Jun Rao, Song Guan, Dingfa Wang
Summary: This study quantified the content of Oroxylin A, a major antifungal compound in Piper sarmentosum extract (PSE), using UPLC-QTOF-MS/MS and investigated its antagonistic activity against Fusarium graminearum and its inhibitory effect on zearalenone (ZEA) production. The results showed that Oroxylin A inhibited both fungal growth and ZEA production in a dose-dependent manner.
Article
Food Science & Technology
Ana Caroline de Oliveira, Vanete Thomaz-Soccol, Michelle Zibetti Tadra-Sfeir, Emanuel Maltempi de Souza, Maria Elena Crespo-Lopez, Herve Rogez
Summary: A new mRNA-based RT-qPCR technique was developed for rapid detection and quantification of viable Trypanosoma cruzi in acai fruits and juice, with a maximum recovery rate of 82.50% and a detection and quantification limit of 10 parasites/mL. The RT-qPCR efficiency was estimated at 97.27%, showing high accuracy in discriminating between viable and nonviable cells.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Medicinal
Xu Ling, Qing-Qing Hao, Christophe Pannecouque, Erik De Clercq, Fen-Er Chen
Summary: This paper reports a study on a novel class of HIV-1 reverse transcriptase inhibitors. By synthesizing 39 derivatives, it was found that these compounds exhibited good inhibitory activity against wild-type and resistant mutant viruses, especially the rilpivirine-associated resistant mutant E138K. Molecular docking analysis provided insights into the biological activity of these compounds and identified potential drug candidates.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Zhao Wang, Waleed A. Zalloum, Wenbo Wang, Xiangyi Jiang, Erik De Clercq, Christophe Pannecouque, Dongwei Kang, Peng Zhan, Xinyong Liu
Summary: Compound 20a, designed based on structural biology information, shows remarkable antiviral activity against HIV-1, low toxicity, high safety index, improved solubility, and promising drug-like properties. It has the potential to be a valuable drug candidate.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Huiyan Lu, Yuji Komukai, Koto Usami, Yan Guo, Xinyue Qiao, Michiyoshi Nukaga, Tyuji Hoshino
Summary: Chemotherapy for human immunodeficiency virus type-1 (HIV-1) has made significant progress, although the emergence of drug-resistant variants remains a serious problem. This study obtained the binding structures of six inhibitory compounds with a recombinant protein of the HIV-1 RNase H domain using X-ray crystal analysis. The compounds were found to bind to the catalytic site and coordinate with two divalent metal ions. These findings provide a solid foundation for developing potent inhibitors for HIV-1 RNase H activity.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Biochemical Research Methods
Thomas Leichtfried, Helga Reisenzein, Siegrid Steinkellner, Richard A. Gottsberger
Summary: The study found that slight modifications of the reverse transcription step in the real-time PCR assay can significantly improve the detection limit, especially for certain plant viruses. This discovery could enhance the sensitivity in detecting viroids and benefit regulatory outcomes for national plant protection organizations.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Biotechnology & Applied Microbiology
M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino, F. Valetti
Summary: The study demonstrates that utilizing autochthonous microbial consortia and high turnover rate hydrogenase genes can efficiently produce bio-hydrogen from green waste and low-value compost. Modulating the expression of hydrogenase genes can optimize dark fermentation process and enhance hydrogen production.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Virology
Come J. Thieulent, Mariano Carossino, Laura Peak, Keith Strother, Wendy Wolfson, Udeni B. R. Balasuriya
Summary: Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in dogs, including SARS-CoV-2. To rapidly detect and differentiate SARS-CoV-2 from other respiratory pathogens from a public health perspective, researchers have developed a multiplex PCR panel that can detect and identify twelve pathogens associated with CIRDC, as well as three CIV subtypes.
Article
Biochemistry & Molecular Biology
Supaphorn Seetaha, Supa Hannongbua, Jatuporn Rattanasrisomporn, Kiattawee Choowongkomon
Summary: The study screened peptides extracted from 111 Asian medicinal plants and found that hydrolyzed peptides from the fruit peel of Quercus infectoria could potentially be developed as inhibitors of HIV-1 RT.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Chemistry, Medicinal
Li Ding, Christophe Pannecouque, Erik De Clercq, Chunlin Zhuang, Fen-Er Chen
Summary: A series of novel heteroaromatic biphenyl-methyl-pyrimidine analogues were designed by hybridizing privileged structures of two HIV-1 inhibitors. Compound 7a showed excellent inhibitory activity against wild-type HIV-1 with low cytotoxicity, but further optimizations were needed to improve its efficacy against mutant strains and pharmacokinetic profiles. Compound 9d, derived from these optimizations, exhibited broad-spectrum activity against resistant strains, good solubility and safety profiles, as well as favorable PK profile, making it a promising drug candidate for HIV clinical therapy.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zhidan Luo, Yong Xue, Xiaoyu Chen, Jian Zhang, Chen Lu
Summary: Researchers successfully constructed three variants of Tth pol with Mn2+-independent reverse transcriptase activity. Among them, the I640F/I709K variant showed good performance in one-step RT-qPCR.
PROTEIN AND PEPTIDE LETTERS
(2023)
Article
Biochemical Research Methods
Hasin Feroz, Daniel Cetnar, Robert Hewlett, Satish Sharma, Melissa Holstein, Sanchayita Ghose, Zheng Jian Li
Summary: Using a in-house RT-qPCR assay for selective detection of active o6 can be a faster, cheaper and safer alternative to third-party testing of BSL2 model viruses. This method can screen VI agents at only 5% of the cost compared to BSL2 virus testing, with a turnaround time of 2 to 3 days.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Medicinal
Chanin Sillapachaiyaporn, Panthakarn Rangsinth, Sunita Nilkhet, Nuntanat Moungkote, Siriporn Chuchawankul
Summary: This study focused on the anti-HIV-1 activity of Curcuma aeruginosa Roxb. extracts, with methanol extract showing the highest potential inhibition of HIV-1 protease. The major compounds in the extracts were identified as fatty acids, amino acids, and terpenoids. In silico investigation revealed strong binding affinities of certain compounds to active residues of both HIV-1 protease and reverse transcriptase, suggesting potential for drug discovery. Additionally, antioxidant activity of the extracts was determined, with ethyl acetate extract showing the highest activity correlated with total phenolic content.
Article
Microbiology
Shuang Wu, Xiaolu Shi, Qiongcheng Chen, Yixiang Jiang, Le Zuo, Lei Wang, Min Jiang, Yiman Lin, Shisong Fang, Bo Peng, Weihua Wu, Hui Liu, Renli Zhang, Patrick S. L. Kwan, Qinghua Hu
Summary: This study compared the specificity and sensitivity of CPA and RT-qPCR kits for SARS-CoV-2 detection, providing a reference for clinical laboratories and informing testing protocols during the rapidly evolving COVID-19 pandemic. The CPA kit showed 100% sensitivity, specificity, PPV/NPV, while some RT-qPCR kits demonstrated good agreement with clinical diagnostic reports and good reproducibility. Differences between all kits were statistically significant.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Virology
Lisa Dettinger, Crystal M. Gigante, Maria Sellard, Melanie Seiders, Puja Patel, Lillian A. Orciari, Pamela Yager, James Lute, Annette Regec, Yu Li, Dongxiang Xia
Summary: The Pennsylvania Department of Health Bureau of Laboratories tested 6855 animal samples for rabies using DFA and LN34 RT-qPCR, with a sensitivity of 0.03%. Two samples initially tested negative by DFA but were positive by LN34 RT-qPCR, which were confirmed positive upon re-testing. The levels of rabies virus in different brain structures varied, with higher abundance observed in more caudal brain structures, suggesting early infection.
Article
Marine & Freshwater Biology
Kannan Maharajan, Sellamani Muthulakshmi, Bojan Nataraj, Mathan Ramesh, Krishna Kadirvelu
AQUATIC TOXICOLOGY
(2018)
Article
Environmental Sciences
Sellamani Muthulakshmi, Kannan Maharajan, Hamid R. Habibi, Krishna Kadirvelu, Mudili Venkataramana
Article
Environmental Sciences
Kannan Maharajan, Sellamani Muthulakshmi, Chinnannan Karthik, Bojan Nataraj, Kanthan Nambirajan, Devan Hemalatha, Swaminathan Jiji, Krishna Kadirvelu, Ke-chun Liu, Mathan Ramesh
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
