Article
Reproductive Biology
Yufei Huang, Xiaoya Chu, Yafei Zhang, Sheng Yang, Yonghong Shi, Jingxian Wu, Qiusheng Chen
Summary: This study investigated the morphological changes in the testis of adult Shaoxing ducks infected with DTMUV. The results showed that DTMUV infection caused significant atrophy in the testis, cytoplasmic vacuolation and exfoliation in the seminiferous epithelium, decrease in the diameter of seminiferous tubule, and interstitial inflammation. Immunohistochemistry confirmed that spermatogenic cells, especially spermatocytes, were the target cells of DTMUV infection in the testis. Single and clustered virus particles were observed in the spermatogenic cells of infected ducks.
Article
Microbiology
Sheng Yang, Yonghong Shi, Jingxian Wu, Qiusheng Chen
Summary: This study systematically investigates the ultrastructural pathology of the central nervous system (CNS) of ducklings and adult ducks infected with Duck Tembusu virus (DTMUV) using transmission electron microscopy technology. The results show that DTMUV causes extensive brain lesions in ducklings and slight damage in adult ducks. Neurons are the target cells of DTMUV, and virions are mainly observed in their endoplasmic reticulum and Golgi apparatus. Additionally, DTMUV infection leads to swelling in astrocytic foot processes in ducklings and myelin lesions in both ducklings and adult ducks.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Huu-Quang Nguyen, Dabin Lee, Yeoseon Kim, Geul Bang, Kun Cho, Young-Sun Lee, Jong Eun Yeon, David M. Lubman, Jeongkwon Kim
Summary: This study utilized LC-MS/MS to analyze the protein expression profiles of extracellular vesicles from serum samples of patients with various fatty liver diseases, revealing significant differences in protein expression levels of apolipoproteins, immunoglobulins, and other markers. Through principal component analysis, a clear differentiation between ALD and NAFLD was achieved. The up-regulation of alpha-2-macroglobulin in alcoholic hepatitis and apolipoprotein C3 in NAFLD were identified as significant findings.
JOURNAL OF PROTEOMICS
(2021)
Article
Veterinary Sciences
Yuhong Pan, Xuedong Wu, Wenjun Cai, Anchun Cheng, Mingshu Wang, Shun Chen, Juan Huang, Qiao Yang, Ying Wu, Di Sun, Sai Mao, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Qun Gao, Xumin Ou, Bin Tian, Zhongqiong Yin, Renyong Jia
Summary: This study utilized high-throughput RNA sequencing to investigate the changes in host gene expression during duck embryo fibroblasts (DEF) infected with Duck Tembusu virus (DTMUV). The results identified a large number of differentially expressed genes related to various biological functions including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. These findings provide valuable insights into the host-DTMUV interaction and have implications for the development of antiviral drugs and vaccines for poultry.
VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Bin Tian, Dongjie Cai, Mingshu Wang, Tianqiong He, Liyao Deng, Liping Wu, Renyong Jia, Dekang Zhu, Mafeng Liu, Shun Chen, Qiao Yang, Ying Wu, Xinxin Zhao, Shaqiu Zhang, Mujeeb Ur Rehman, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Xinjian Wen, Di Sun, Yanling Yu, Ling Zhang, Yunya Liu, Leichang Pan, Xiaoyue Chen, Anchun Cheng
Summary: The study demonstrated that SC75741 exhibits strong antiviral activity against Duck Tembusu virus and Duck plague virus by blocking viral entry and genomic replication, making it a potential antiviral agent for DTMUV and DPV.
Article
Agriculture, Dairy & Animal Science
Juan Huang, Lin Lei, Min Cui, Anchun Cheng, Mingshu Wang, Mafeng Liu, Dekang Zhu, Shun Chen, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Zhongqiong Yin, Renyong Jia
Summary: miR-146b-5p regulates replication of DTMUV by targeting RPS14.
Article
Biochemistry & Molecular Biology
Aleksandra E. Lewandowska, Anna Fel, Marcel Thiel, Paulina Czaplewska, Krzysztof Lukaszuk, Jacek R. Wisniewski, Stanislaw Oldziej
Summary: Two label-free quantitative workflows based on different high-resolution mass spectrometers and LC setups were presented for the analysis of human follicular fluid proteome, with around 1000 proteins identified in each workflow. The concentration values obtained from both workflows showed significant correlation, leading to unified lists of proteins linked to oocyte maturity and blastocyst development. The Quad-Orbitrap workflow is suitable for in-depth analysis of low abundant proteome without extensive fractionation, while the Triple Quad-TOF workflow offers a more robust approach with potential for effectiveness with increasing number of analyzed samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Yunzhen Huang, Ziqiang Hu, Jiawen Dong, Linlin Li, Junqin Zhang, Ruihuan Kuang, Shimin Gao, Minhua Sun, Ming Liao
Summary: In this study, a chicken-origin TMUV strain was isolated from laying hens with egg drop syndrome in south China. The results showed that this strain exhibited high infectivity and pathogenicity in both chicks and ducklings, suggesting its potential for widespread infection of chickens and ducks.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Alessio Di Luca, Francesca Bennato, Andrea Ianni, Lisa Grotta, Michael Henry, Paula Meleady, Giuseppe Martino
Summary: In this study, proteomic technologies were used to compare the milk proteome of two goat breeds, Teramana and Saanen. The analysis identified 29 proteins that could discriminate between the two breeds. This research contributes to our understanding of the goat milk proteome and provides important information for future studies on goat milk proteins.
Article
Immunology
Tianxu Li, Yanyan Ren, Tingting Zhang, Xinyu Zhai, Xiuyuan Wang, Jinchao Wang, Bin Xing, Runchun Miao, Ning Li, Liangmeng Wei
Summary: This study investigates the regulatory role of duLGP2 in the duck RIG-I-mediated antiviral innate immune signaling system. The results demonstrate that duLGP2 can suppress duRIG-I activation and enhance the production of inflammatory cytokines. These findings contribute to a better understanding of the regulatory networks of the antiviral innate immune system in ducks.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Xuemei Xian, Sheng Yang, Yonghong Shi, Qiusheng Chen
Summary: In this study, it was found that the Duck Tembusu virus (DTMUV) enters cells through the pathway of vesicular endocytosis, and undergoes maturation and replication in the rough endoplasmic reticulum (RER) cisternae. The virus particles are then released from the infected cells after reaching the cell membrane via the Golgi body's endocrine channel. The entry and replication of DTMUV in mammalian and avian cells differ, and can be blocked by a clathrin inhibitor, chlorpromazine (CPZ).
Article
Microbiology
Sheng Chen, Yiming Yan, Liguo Gao, Shuang Gao, Keyu Feng, Hongxin Li, Xinheng Zhang, Weiguo Chen, Feng Chen, Qingmei Xie
Summary: In this study, proteomic analysis revealed that 53 cellular proteins are incorporated into mature ALV-J virions, with potential functions in the viral life cycle and tumorigenesis. These findings provide new insights into the interaction between ALV-J and its host, and shed light on the cellular requirements for ALV-J infection.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Sheng Yang, Jingxian Wu, Yufei Huang, Yafei Zhang, Yonghong Shi, Ping Yang, Qiusheng Chen
Summary: This study constructed a DTMUV-infected duckling model to investigate apoptosis in the duckling brains. It was found that DTMUV infection could induce apoptosis in the brains of ducklings, and the occurrence of apoptosis was accompanied by the virus infection process with certain regularity.
Article
Immunology
Peng Zhou, Lei Ma, Zaixiao Rao, Yaqian Li, Huijun Zheng, Qigai He, Rui Luo
Summary: The Duck Tembusu virus (DTMUV) is a pathogenic flavivirus that impacts egg production and neurological disorders in waterfowl. Proteomics analysis revealed duIFI35 expression promotes DTMUV replication and inhibits IFN-beta production, disrupting the host antiviral response. Specifically, duIFI35 impairs duRIG-I recognition of double-stranded RNA, evading the immune response and providing insights for antiviral drug design.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Wikanda Tunterak, Duangduean Prakairungnamthip, Patchareeporn Ninvilai, Sonthaya Tiawsirisup, Kanisak Oraveerakul, Jiroj Sasipreeyajan, Alongkorn Amonsin, Aunyaratana Thontiravong
Summary: DTMUV, a mosquito-borne flavivirus, has emerged as a causative agent of viral disease in ducks in Thailand, with infections occurring year-round and varying patterns between farm and free-grazing duck systems, peaking in the winter season. Routine surveillance is essential for early detection and control measures.