期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 127, 期 -, 页码 241-246出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.01.047
关键词
Biosensor; Liquid crystal droplet; Microfluidics; Biotin; Avidin; Selective biosensor
资金
- National Research Foundation of Korea [NRF-2011-0020264]
- [NRF-2014R1A2A1A11050451]
Poly(acrylicacid-b-4-cynobipheny1-4'-undecylacrylate) (PAA-b-LCP)-functionalized 4-cyano-4'-pentylbiphenyl (5CB) droplets were made by using microfluidic technique. The PAA chains on the 5CB droplets, were biotinylated, and used to specifically detect avidin-biotin binding at the 5CB/aqueous interface. The avidin-biotin binding was characterized by the configurational change (from radial to bipolar) of the 5CB droplets, as observed through a polarized optical microscope. The maximum biotinylation was obtained by injecting a >100 mu g/mL biotin aqueous solution, which enabled a limit of detection of 0.5 mu g/mL avidin. This droplet biosensor could specifically detect avidin against other proteins such as bovine serum albumin, lysozyme, hemoglobin, and chymotrypsinogen solutions. Avidin detection with 5CB(PAA-biotin) droplets having high sensitivity, specificity, and stability demonstrates new applications of the functionalized liquid crystal droplets that can detect specific proteins or other analytes through a ligand/receptor model. (C) 2015 Elsevier B.V. All rights reserved.
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