期刊
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
卷 18, 期 6, 页码 751-759出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6em00120c
关键词
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资金
- Special Scientific Research Fund of Public Welfare of Environmental Protection
- Ministry of Environmental Protection of China [201309001]
- National Science and Technology Major Project on Water Pollution Control and Treatment [2011ZX07301-003]
Here, we constructed an Escherichia coli recA:: luxCDABE bioreporter for genotoxicity assessments. The recA promoter was cloned from the marine bacterium Vibrio natriegens. This bioreporter showed a dose-response relationship following induction by mitomycin C, and other pollutants or environmental samples could be calculated as mitomycin C equivalents, which provided a way to quantitatively compare the genotoxicities of different environmental samples. This bioreporter was used to evaluate the genotoxicity under a wide range of external environmental conditions, like temperatures ranging from 15 degrees C to 42 degrees C, pH between 4.0 and 9.0, and salinity ranging from 0% to 3%. This successfully extended its application from the laboratory to the field, and allowed the bioreporter to assess the genotoxicity and bioavailability of genotoxins in various environmental media, including surface water, groundwater, seawater, and soil matrix. Expression of V. natriegens recA in E. coli indicated a LexA-like regulator in V. natriegens, and the putative SOS box of V. natriegens recA was similar to that of E. coli. The genotoxicities of groundwater samples from a petrochemical-contaminated site in northern China were evaluated by this bioreporter assay, and the genotoxic levels were in accordance with contamination levels obtained by chemical analyses.
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