Controlled delivery of dopamine hydrochloride using surface modified carbon dots for neuro diseases

Delivery of therapeutic agents using water-soluble, highly biocompatible Carbon dots (C-dots) is an efficient strategy to control drug release under physiological milieu. Dopamine hydrochloride (DA), the most important inotropic vasopressor agent used in neurological diseases. In our study DA is anchored to water-soluble carbon dots for controlled release under mimicked in vitro physiological conditions. The tenure of the DA release at pH 7.4 was greatly extended to 60 h for C-dots-DA, in comparison with the control DA alone. The statistical calculation was used to comprehend the release pattern of the DA, which exhibited the pattern of Hixson-Crowell model of release. In order to understand the impact of the C-dots-DA conjugate under physiological conditions, Neuro 2A cells were taken under consideration. The conjugate C-dots-DA was found to be biocompatible against Neuro 2A cells. The survival rate was found to be 74% at maximum concentration of 9 mu g mL(-1). In vivo toxicity was studied using thin section of tissues after staining with Hematoxyline and Eosin Yellow (H&E). As per microscopic observations, conjugates did not inflict any anatomical distortions or hostile effects on tissues. Body weight of mice was also taken into consideration after injecting 20 mu g mL(-1) of nano-conjugates via tail vein. The impact of nano-conjugate on body weight was found to be negligible after 45 days of observation. (C) 2015 Elsevier B.V. All rights reserved.