Article
Virology
Tianshi Xiao, Xiaolin Zhu, Wenhui Wang, Xuchen Jia, Changmei Guo, Xue Wang, Zhihui Hao
Summary: In this study, 52 phages were isolated from 816 samples from pig, chicken, and duck farms in China. A novel Escherichia phage, vB_EcoStr-FJ63A, was identified from pig feces, which showed lytic activity against colistin-resistant strains of E. coli. Further research is needed to develop vB_EcoStr-FJ63A as a potential biocontrol agent against colistin-resistant E. coli.
Article
Microbiology
Petra Sismova, Iva Sukkar, Nikita Kolidentsev, Jana Palkovicova, Ivana Chytilova, Jan Bardon, Monika Dolejska, Kristina Nesporova
Summary: This study investigated the occurrence of plasmid-mediated colistin resistance in domestic and imported meat and slaughter animals in the Czech Republic. The results showed a high prevalence of mcr genes in fresh meat samples, especially in poultry. The study highlights the importance of surveillance and the potential risk of transmission of these resistant bacteria to humans through the food chain.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Dusan Misic, Ferenc Kiskaroly, Michael P. Szostak, Adriana Cabal, Werner Ruppitsch, Tanja Bernreiter-Hofer, Viktoria Milovanovic, Andrea T. Fessler, Franz Allerberger, Joachim Spergser, Elke Mueller, Stefan Schwarz, Sascha D. Braun, Stefan Monecke, Ralf Ehricht, Maciej Korus, Damir Benkovic, Malgorzata Korzeniowska, Igor Loncaric
Summary: This study aimed to monitor the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry in intensive breeding facilities in Northern Vojvodina, Serbia. The study identified mcr-1 gene in E. coli isolates from healthy turkeys, with various ST types and virulence-associated genes. The isolates showed susceptibility to tested biocides, indicating no resistance. Continuous monitoring of livestock samples is advised to prevent potential transmission to humans.
Article
Chemistry, Multidisciplinary
Jakob Andersson, Melanie Fuller, Alex Ashenden, Stephen A. Holt, Ingo Koper
Summary: Antibiotic resistance is expected to be a major challenge for healthcare systems in the future, with gold nanoparticles showing the potential to enhance bacterial susceptibility to antibiotics, providing a more effective treatment for drug-resistant infections.
Article
Engineering, Biomedical
Chengyuan Qin, Ning Tang, Yingying Gan, Huimin Zhao, Yuzhen Li, Guo-bao Tian, Yi Yan Yang, Peiyan Yuan, Xin Ding
Summary: This study discovered that curcumin can reverse colistin-resistant bacteria and prepared negatively charged poly(ethylene glycol)-functionalized liposomes encapsulating both curcumin and colistin, which showed stronger antibacterial activity against colistin-resistant bacteria than the combination of free curcumin and colistin. Mechanistical studies revealed that the liposomes restored the affinity of colistin for the bacterial membrane and improved the uptake of curcumin, leading to reduced efflux pump activity and achieving a synergistic effect. In addition, the liposomes showed no toxicity at effective antibacterial doses and demonstrated therapeutic efficacy in an intestinal bacterial infection model.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Veterinary Sciences
Wiebke Jansen, Jobke van Hout, Jeanine Wiegel, Despoina Iatridou, Ilias Chantziaras, Nancy De Briyne
Summary: This study investigated the use patterns of colistin in veterinary medicine in different European countries and sectors. The majority of veterinarians surveyed did not use or decreased their use of colistin, with gastrointestinal diseases and septicaemia being the main indications for use. Some veterinarians reported governmental/industry restrictions on colistin use, with the most common restriction being the requirement of susceptibility testing before use.
VETERINARY SCIENCES
(2022)
Review
Infectious Diseases
Jitka Rychlickova, Vendula Kubickova, Pavel Suk, Karel Urbanek
Summary: The emerging resistance of Gram-negative bacteria is a global problem that has led to the reintroduction of colistin as a therapeutic option. However, there are still many questions regarding the safety and efficacy of colistin. This review summarizes the available literature on the use of colistin in critically ill patients, focusing on stability, pharmacokinetics, plasma concentration determination methods, and therapeutic drug monitoring benefits and limitations. Based on these findings, the gaps in knowledge are identified, and future research directions are suggested.
Review
Veterinary Sciences
George Valiakos, Ioanna Kapna
Summary: This review gathered and presented research results on colistin resistance due to mcr genes prevalence in livestock animals on an international level, highlighting the widespread dispersion of mcr genes to various animal species and the need for enhanced control measures.
VETERINARY SCIENCES
(2021)
Article
Microbiology
Nikolaos Strepis, Anne F. Voor In't Holt, Margreet C. Vos, Willemien H. A. Zandijk, Astrid P. Heikema, John P. Hays, Juliette A. Severin, Corne H. W. Klaassen
Summary: The study retrospectively characterized the genetic composition and epidemiology of plasmids from mcr-1-positive bacterial isolates from a large academic hospital in the Netherlands. Results showed that mcr-1-carrying plasmids had diverse genetic backgrounds, involving different types of plasmids, and transmission events occurred within patients, between patients, and across different species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Agnes Sonnevend, Walid Q. Alali, Sara A. Mahmoud, Akela Ghazawi, Greeshma Bharathan, Szilvia Melegh, Tahir A. Rizvi, Tibor Pal
Summary: Data on the prevalence of MCR-producing Enterobacterales of animal origin are scarce from the Arabian Peninsula. We investigated these strains from poultry fecal specimens in the United Arab Emirates and found mcr-1.1 positive strains in 36 out of 40 samples. Whole genome sequencing revealed the resistance and virulence genes, MLST and cgMLST of 39 selected isolates. Transferability and incompatibility types of the MCR-plasmids were also determined. The diversity of mcr-1 positive strains indicates the need for coordinated surveillance including animals, retail meat and clinical cases.
Article
Microbiology
P. S. Seethalakshmi, Riya Rajeev, Anushara Prabhakaran, George Seghal Kiran, Joseph Selvin
Summary: Colistin resistance in bacteria poses a significant threat to public health, as it is often the last line of defense against multidrug resistant pathogens. The emergence of colistin resistance in aquaculture and poultry settings has heightened concerns about its spread in the environment. The coexistence of colistin resistant genes with other antibiotic resistant genes complicates efforts to combat antimicrobial resistance. To address this issue, a comprehensive approach that encompasses human, animal, and environmental health is necessary.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Immunology
Yanling Liu, Yiqing Lin, Ziwen Wang, Niya Hu, Qiong Liu, Wenkai Zhou, Xiuzhen Li, Longhua Hu, Jian Guo, Xiaotian Huang, Lingbing Zeng
Summary: The prevalence of colistin-resistant Klebsiella pneumoniae with mcr genes is increasing in clinical settings, while the emergence of hypervirulent and multidrug-resistant strain ST11 K. pneumoniae highlights the importance of active surveillance in hospitals. The study revealed the widespread presence of mobile colistin-resistant genes and genetic diversity among the COLR-KP isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Nian Zhang, Wenying Shan, Liangliang Gao, Si Hoi Kou, Chang Lu, Huilin Yang, Bo Peng, Kin Yip Tam, Leo Tsz On Lee, Jun Zheng
Summary: The emergence and spread of drug-resistant bacterial pathogens, such as ESKAPE, present a serious global health threat. However, the development of new antibiotics is hindered by challenges in identifying new drug targets and the rapid development of resistance. Drug repurposing, such as the use of BMS-833923, offers an effective strategy to combat antibiotic resistance by enhancing the efficacy of existing antibiotics. BMS-833923 kills Gram-positive bacteria and potentiates colistin to destroy various Gram-negative bacteria by targeting membrane phospholipids, ultimately causing cell death.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Medicine, Research & Experimental
Lamiaa A. Al-Madboly, Ahmed A. Abdelaziz, Amal M. Abo-Kamer, Ahmed M. Nosair, Karim Abdelkader
Summary: In this study, the therapeutic capacity of the Klebsiella bacteriophage NK20 against pandrug-resistant strains was investigated. It was found that NK20 showed a relatively broad spectrum and an acceptable genomic profile. Despite the development of bacterial resistance, intranasal administration of NK20 significantly reduced the bacterial load in both the pulmonary and blood compartments and rescued the infected rats.