期刊
BIOTECHNOLOGY JOURNAL
卷 11, 期 5, 页码 708-716出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201400565
关键词
Continuous aqueous two-phase system; Immunoglobulin G; Microfluidic screening; Mixer-settler device; Multistage extraction
资金
- Bioprocess and Synthetic Biology Research Group of Tecnologico de Monterrey
- CONACYT [246739]
Aqueous two-phase systems (ATPS) are a liquid-liquid extraction technology with clear process benefits; however, its lack of industrial embracement is still a challenge to overcome. Antibodies are a potential product to be recovered by ATPS in a commercial context. The objective of this work is to present a more integral approach of the different isolated strategies that have arisen in order to enable a practical, generic implementation of ATPS, using human immunoglobulin G (IgG) as experimental model. A microfluidic device is used for ATPS parameters preselection for product recovery. ATPS were continuously operated in a mixer-settler device in one stage, multistage and multistage with recirculation configuration. Single-stage pure IgG extraction with a polyethylene glycol (PEG) 3350-phophates ATPS within continuous operation allowed a 65% recovery. Further implementation of a multistage platform promoted a higher particle partitioning reaching a 90% recovery. The processing of IgG from a cell supernatant culture harvest in a multistage system with top phase recirculation resulted in 78% IgG recovery in bottom phase. This work conjugates three not widely spread methodologies for ATPS: microfluidics, continuous and multistage operation.
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