Article
Reproductive Biology
Feng Chen, Yingjie Wu, Lu Ke, Xinyi Lin, Fengchao Wang, Yinghe Qin
Summary: This study revealed that specific deletion of Arhgef15 in Sertoli cells of mice leads to sperm abnormality, disruption of BTB integrity, and potentially affects testicular immune homeostasis.
BIOLOGY OF REPRODUCTION
(2022)
Review
Cell Biology
Mingxia Yao, Haoyang Qu, Yating Han, C. Yan Cheng, Xiang Xiao
Summary: This review summarizes recent findings on the role of Kinesin in mammalian spermatogenesis, highlighting its potential role in germ cell traversal through the blood-testis barrier and the remodeling of Sertoli cell-spermatid junctions to advance spermatid transport. The possibility of Kinesin acting as a mediator and/or synchronizer for cell cycle progression, germ cell transit, and junctional rearrangement and turnover is also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Obstetrics & Gynecology
Sulagna Dutta, Narpal Sandhu, Pallav Sengupta, Marco G. Alves, Ralf Henkel, Ashok Agarwal
Summary: The development of antibodies to antigens involved in sperm production can lead to autoimmune responses affecting sperm motility, functions, and fertility. The testes need to establish an immune-privileged microenvironment to protect germ cells through various mechanisms.
REPRODUCTIVE SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Yi Zheng, Qiang Gao, Tianjiao Li, Ruifang Liu, Zechao Cheng, Ming Guo, Jinhong Xiao, De Wu, Wenxian Zeng
Summary: The study systematically investigated the development patterns of Sertoli cells and spermatogonia in pigs from neonate to sexual maturity, shedding light on previously unknown details. The findings not only expand knowledge about spermatogenesis and testicular development in pigs, but also provide a theoretical basis for porcine breeding and rearing.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Review
Endocrinology & Metabolism
Wasim Shah, Ranjha Khan, Basit Shah, Asad Khan, Sobia Dil, Wei Liu, Jie Wen, Xiaohua Jiang
Summary: The process of spermatogenesis relies on hormones and growth factors acting through endocrine and paracrine pathways. Sertoli cells are major regulators, while sex hormones play a significant role in determining their proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell Biology
Rachel L. Washburn, Taylor Hibler, Lea Ann Thompson, Gurvinder Kaur, Jannette M. Dufour
Summary: Sertoli cells, as immune privileged cells in the testis, provide immune protection to maturing germ cells. They have potential therapeutic applications in creating tolerogenic environments, protecting co-grafted cells, and delivering therapeutic proteins through gene therapy. Studies have shown their potential use in treating diabetes, neurodegenerative disorders, and restoration of spermatogenesis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou
Summary: The study found that testicular stromal cells, including Sertoli, endothelial, Leydig, smooth muscle, and Tcf21-CreER+ stromal cells, express stem cell factor (SCF), an essential growth factor for spermatogenesis. Undifferentiated and differentiating spermatogonia were associated with SCF-expressing Sertoli cells. Conditional deletion of SCF from Sertoli cells blocked the differentiation of spermatogonia, leading to male infertility. Conditional overexpression of SCF in Sertoli cells significantly increased spermatogenesis.
Article
Biochemistry & Molecular Biology
Siwen Wu, Linxi Li, Xiaolong Wu, Chris K. C. Wong, Fei Sun, C. Yan Cheng
Summary: AKAP9 is a crucial regulatory protein in rat testes, supporting spermatogenesis by regulating MT and actin polymerization. Its function in Sertoli cells is essential for maintaining cytoskeleton homeostasis and adhesion between Sertoli cells and GC in the testis.
Article
Cell & Tissue Engineering
Nicole Parker, Andrew Laychur, Meena Sukwani, Kyle E. Orwig, Jon M. Oatley, Chao Zhang, Florentine U. Rutaganira, Kevan Shokat, William W. Wright
Summary: This study investigates the replication and growth of SSCs by temporarily inhibiting GDNF signaling in adult mice. The results show that after restoring normal signaling, SSCs are able to self-renew and gradually restore their numbers. There are important regulatory interactions among SSCs and progenitor cells.
Review
Endocrinology & Metabolism
Juho-Antti Makela, Jorma Toppari
Summary: The RB protein family members play crucial roles in cell cycle progression, apoptosis, and stem cell self-renewal and differentiation. RB proteins exert their effects through interaction with E2F transcription factors. RB and E2F family members are widely and dynamically expressed in the testis and have versatile roles during spermatogenesis. RB-E2F interaction is critical for male fertility and proper germline maintenance and lifelong sperm production.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Endocrinology & Metabolism
Maia Corpuz-Hilsabeck, Martine Culty
Summary: Sertoli cells play crucial roles in male reproduction, and dysregulation of their functions can have lifelong adverse effects on testis development and spermatogenesis. Understanding the impact of endocrine disrupting chemicals and drugs on Sertoli cells is important for comprehending the adverse outcomes on the reproductive system that these compounds may induce.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Immunology
Yue Yang, Mingxia Yao, Jie Zeng, Dongwang Zheng, Qin Li, Ya Ni, Xiang Xiao
Summary: FYN plays an important role in maintaining the integrity of the blood-testis barrier and the adhesion of germ cells to Sertoli cells. It is structurally linked to the actin and microtubule-based cytoskeletons. The study suggests that FYN and Arp3 form a protein complex that regulates junction reorganization events at the apical ectoplasmic specialization and the blood-testis barrier, and this mechanism may also allow viruses to enter the immunoprivileged testicular microenvironment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kristina Rode, Marion Langeheine, Bettina Seeger, Ralph Brehm
Summary: Studies have shown that Cx43 in spermatogonia/spermatids is not essential for successful termination of spermatogenesis, and using a GC-specific Cx43 KO does not affect fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Won -Yong Lee, Hyun-Jung Park
Summary: In this study, the toxicity of CeO(2)NPs on mouse testes was evaluated using in vitro organ cultures. The results showed that CeO(2)NPs could reduce the number of germ cells in a dose-dependent manner and disturb the function of Sertoli cells and steroidogenesis.
REPRODUCTIVE TOXICOLOGY
(2022)
Article
Endocrinology & Metabolism
Elizabeth Tang, C. Yan Cheng
Summary: Microtubule affinity-regulating kinases (MARKs) play an important role in the Sertoli cell blood-testis barrier, regulating cell polarity, microtubule dynamics, and tight junction permeability. MARK2 and MARK4 have different effects on the blood-testis barrier and MT cytoskeleton, with MARK4 being more severe.
Article
Endocrinology & Metabolism
Yu Qin, Jennifer R. Wang, Ying Wang, Priyanka Iyer, Gilbert J. Cote, Naifa L. Busaidy, Ramona Dadu, Mark Zafereo, Michelle D. Williams, Renata Ferrarotto, G. Brandon Gunn, Peng Wei, Keyur Patel, Marie-Claude Hofmann, Maria E. Cabanillas
Summary: This study demonstrates the value of cfDNA gene mutation analysis in aiding early diagnosis and treatment selection for ATC patients. High concordance rates were observed between the mutations detected in cfDNA and those from tumor tissue sequencing. Patients with a PIK3CA mutation in cfDNA had worse overall survival, while BRAFi therapy significantly improved survival outcomes for ATC patients.
Article
Endocrinology & Metabolism
Anastasios Maniakas, Ying C. Henderson, Hu Hei, Shaohua Peng, Yunyun Chen, Yujie Jiang, Shuangxi Ji, Maria Cardenas, Yulun Chiu, Diana Bell, Michelle D. Williams, Marie-Claude Hofmann, Steve E. Scherer, David A. Wheeler, Naifa L. Busaidy, Ramona Dadu, Jennifer R. Wang, Maria E. Cabanillas, Mark Zafereo, Faye M. Johnson, Stephen Y. Lai
Summary: This study developed and characterized 6 novel ATC PDX models with 4 matching cell lines, each with distinct genetic profiles. The PDX models showed remarkable resemblance to the original patient surgical specimens and had strong concordance with the original tumor in terms of genetic characteristics. These models can serve as excellent tools for preclinical therapeutic trials.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Editorial Material
Andrology
Marie-Claude Hofmann, Aleksander Giwercman
Editorial Material
Andrology
Marie-Claude Hofmann, Aleksander Giwercman
Review
Endocrinology & Metabolism
Marie-Claude Hofmann, Elena McBeath
Summary: This review focuses on the activation of master regulators of the niche in Sertoli cells and their targets, as well as novel molecular mechanisms underlying the regulation of growth and differentiation factors through NOTCH signaling and other pathways.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Oncology
Marie-Claude Hofmann, Muthusamy Kunnimalaiyaan, Jennifer R. Wang, Naifa L. Busaidy, Steven Sherman, Stephen Y. Lai, Mark Zafereo, Maria E. Cabanillas
Summary: Protein kinases play critical roles in cell survival, proliferation, and motility, and their dysregulation is common in solid tumors like thyroid cancer. Inhibiting activated protein kinases has been a breakthrough in thyroid cancer therapy, but drug resistance remains a challenge that needs to be overcome with new strategies.
ENDOCRINE-RELATED CANCER
(2022)
Review
Obstetrics & Gynecology
Lars Bjorndahl, Christopher L. R. Barratt, David Mortimer, Ashok Agarwal, Robert J. Aitken, Juan G. Alvarez, Natalie Aneck-Hahn, Stefan Arver, Elisabetta Baldi, Lluis Bassas, Florence Boitrelle, Riana Bornman, Douglas T. Carrell, Jose A. Castilla, Gerardo Cerezo Parra, Jerome H. Check, Patricia S. Cuasnicu, Sally Perreault Darney, Christiaan de Jager, Christopher J. De Jonge, Joel R. Drevet, Erma Z. Drobnis, Stefan S. Du Plessis, Michael L. Eisenberg, Sandro C. Esteves, Evangelini A. Evgeni, Alberto Ferlin, Nicolas Garrido, Aleksander Giwercman, Ilse G. F. Goovaerts, Trine B. Haugen, Ralf Henkel, Lars Henningsohn, Marie-Claude Hofmann, James M. Hotaling, Piotr Jedrzejczak, Pierre Jouannet, Niels Jorgensen, Jackson C. Kirkman Brown, Csilla Krausz, Maciej Kurpisz, Ulrik Kvist, Dolores J. Lamb, Hagai Levine, Kate L. Loveland, Robert McLachlan, Ali Mahran, Liana Maree, Sarah Martins da Silva, Michael T. Mbizvo, Andreas Meinhardt, Roelof Menkveld, Sharon T. Mortimer, Sergey Moskovtsev, Charles H. Muller, Maria Jose Munuce, Monica Muratori, Craig Niederberger, Cristian O'Flaherty, Rafael Oliva, Willem Ombelet, Allan A. Pacey, Michael A. Palladino, Ranjith Ramasamy, Liliana Ramos, Nathalie Rives, Eduardo Rs Roldan, Susan Rothmann, Denny Sakkas, Andrea Salonia, Maria Cristina Sanchez-Pozo, Rosanna Sapiro, Stefan Schlatt, Peter N. Schlegel, Hans-Christian Schuppe, Rupin Shah, Niels E. Skakkebaek, Katja Teerds, Igor Toskin, Herman Tournaye, Paul J. Turek, Gerhard van der Horst, Monica Vazquez-Levin, Christina Wang, Alex Wetzels, Theodosia Zeginiadou, Armand Zini
Summary: Biomedical science is making rapid progress in terms of transparency, openness, and reproducibility of scientific publications, particularly in studies based on human semen examination. Two recent documents, the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, and the International Standard ISO 23162:2021, provide guidelines for authors to follow these laboratory methods and use a checklist to ensure the reliability of their studies when publishing in peer-reviewed journals.
HUMAN REPRODUCTION
(2022)
Editorial Material
Andrology
Aleksander Giwercman, Marie Claude Hofmann
Article
Andrology
Ewa Rajpert-De Meyts, Douglas T. Carrell, Manuela Simoni, Aleksander Giwercman, Marie-Claude Hofmann
Review
Andrology
Robert John Aitken, Sheena E. M. Lewis
Summary: This review summarizes the causes and consequences of DNA damage in the male germ line, including how DNA integrity is maintained in stem cells and the increase in background mutation rates with paternal age. It explains the repair pathways activated in germ cells and spermatozoa in response to genetic damage, as well as the potential mutagenic effects of such damage on offspring. The strategies for managing high levels of DNA damage in spermatozoa to reduce risks to offspring health are also discussed.
Article
Oncology
Madison M. Rose, Veronica L. Espinoza, Katelyn J. Hoff, Laura A. Pike, Vibha Sharma, Marie-Claude Hofmann, Aik Choon Tan, Nikita Pozdeyev, Rebecca E. Schweppe
Summary: Thyroid cancer is a common malignancy with limited treatment options. Inhibition of Src and MAPK pathway can enhance anti-tumor effects, and BIM may serve as a potential biomarker of response.
Review
Biochemistry & Molecular Biology
Elena McBeath, Keigi Fujiwara, Marie-Claude Hofmann
Summary: Until recently, the methods for generating floxed mice have been challenging, expensive, error-prone, or time-consuming. To overcome these issues, some labs have successfully used a small artificial intron to conditionally knockout a gene of interest in mice. However, others have encountered difficulties with the technique, primarily due to incorrect splicing or insufficient knockout of the gene's protein. This article presents a guide on selecting the appropriate exon and placing the recombinase-regulated artificial intron to prevent splicing disruption and maximize mRNA degradation. Following these recommendations should increase the success rate of producing tissue-specific knockout mice using this alternative technique.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Xiao Zhao, Jennifer Rui Wang, Ramona Dadu, Naifa Lamki Busaidy, Lei Xu, Kim O. Learned, Noah N. Chasen, Thinh Vu, Anastasios Maniakas, Arturo A. Eguia, Julia Diersing, Neil D. Gross, Ryan Goepfert, Stephen Y. Lai, Marie-Claude Hofmann, Renata Ferrarotto, Charles Lu, Gary Brandon Gunn, Michael T. Spiotto, Vivek Subbiah, Michelle D. Williams, Maria E. Cabanillas, Mark E. Zafereo
Summary: This study aimed to describe the outcomes of patients with BRAF(V600E)-mutated ATC who received neoadjuvant BRAF-directed therapy followed by surgery. The results showed that neoadjuvant therapy reduced the extent of surgery and surgical morbidity, and patients treated with neoadjuvant therapy had higher overall survival and progression-free survival rates compared to those who didn't have surgery or had surgery before therapy.
Review
Endocrinology & Metabolism
Sarah Hamidi, Marie-Claude Hofmann, Priyanka C. C. Iyer, Maria E. E. Cabanillas, Mimi I. I. Hu, Naifa L. L. Busaidy, Ramona Dadu
Summary: The treatment of advanced, radioiodine refractory, differentiated thyroid cancers (RR-DTCs) has seen significant advancements in the past decade, leading to a paradigm shift in patient management and prognosis. Improved understanding of tumor molecular drivers and access to next generation sequencing have resulted in the development and FDA-approval of targeted therapies for RR-DTCs, including multikinase inhibitors and fusion-specific kinase inhibitors. However, current treatments are not curative, and research efforts are focused on identifying and overcoming resistance mechanisms to tyrosine kinase inhibitors.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Meeting Abstract
Oncology
Muthusamy Kunnimalaiyaan, Parag Parekh, Jalyn Golden, Ying Anderson, Stephen Lai, Maria Cabanillas, Marc Zafereo, Ramona Dadu, Marie-Claude Hofmann
MOLECULAR CANCER THERAPEUTICS
(2021)
