期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 16, 期 -, 页码 473-480出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2016.04.007
关键词
Algae; ATP; Growth; Biofuel; Biomass; RNAi
资金
- Rutgers Start-up and Research Council Grant [202270]
- NIH [1R15GM093685-01]
The green algae, Chlamydomonas reinhardtii, is currently one of the most targeted organisms for renewable biofuel production, yet its growth depends strictly on warm (subtropical) conditions. This requirement makes C. reinhardtii not only susceptible to cold stress, but also unsuitable for culturing large scale algae year round in temperate regions. Using RNAi mediated gene knockdown, we targeted the AMP degradation enzyme, AMP deaminase (AMPD), to reflect psychrophilic-like purine metabolism (purine metabolism in cold adapted organisms) in C. reinhardtii. The engineered C. reinhardtii strains elevated intracellular steady-state ATP levels by more than twofold across their viable temperature range, displayed similar to 3-fold higher growth rate and biomass, and similar to 25% higher lipid/oil accumulation compared to wild-type under both normal and cold growth conditions. Our findings can be extrapolated to engineer plants that grow under cold stress conditions for sustainable agriculture and plant-based biofuel production. (C) 2016 Elsevier B.V. All rights reserved.
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