期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 20, 期 -, 页码 118-125出版社
ELSEVIER
DOI: 10.1016/j.algal.2016.10.004
关键词
Photonfluxostat; Cyanobacteria; Growth rate; Cell size; Light limitation; Synechocystis
资金
- China Scholarship Council [201304910183]
- Dutch Ministry of Economic Affairs, Agriculture, and Innovation (research program BioSolarCells)
- Netherlands Organization for Scientific Research (NWO) [864.11.011, 863.11.019]
The growth rate and physiology of photoautotrophic bacteria are dependent on the incident light color and intensity. Here we report a widely applicable and straightforward method for light-limited batch cultivation of phototrophic bacteria at different, yet constant, growth rates. We illustrate its usage with Synechocystis sp. PCC6803, a model cyanobacterium used as a chassis for sustainable cell-factories and capable of turning CO2 into commodity products. The cultivationmethodwe developed resembles a 'photonfluxostat'. It enables the setting of the growth rate of phototrophs during batch cultivation by adjustment of the illumination intensity ('photon dosing'). Using this method to study the growth-rate response of Synechocystis, we found that while the cell volumeincreased, the chlorophyll a content and the PSI/PSII ratio decreased, as growth rate increased. This method allows for a quantitative and controlled study of the light-dependent physiology of phototropic bacteria, a highly relevant group of bacteria for modern biotechnology. (C) 2016 The Authors. Published by Elsevier B.V.
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