Article
Chemistry, Multidisciplinary
Emily C. Sanders, Sanjana R. Sen, Aidan A. Gelston, Alicia M. Santos, Xuan Luo, Keertna Bhuvan, Derek Y. Tang, Colin L. Raston, Gregory A. Weiss
Summary: Unlocking the potential of personalized medicine in point-of-care settings requires the development of a new generation of biomarker and proteomic assays. A newly developed immunoassay method shows efficient, robust, and cost-effective characteristics, making it applicable in both laboratory and clinical settings.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
[Anonymous]
Summary: The vortex fluidic device accelerated immunoblot assay (VAIA) is a new method that significantly reduces the processing time of immunoblot from nearly 2 hours to less than 5 minutes. Gregory A. Weiss and his colleagues demonstrate the wide applicability and potential of VAIA in their Communication.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Food Science & Technology
Alireza Ebrahimi, Gholamreza Pazuki, Mehrdad Mozaffarian, Farzaneh Ghazizadeh Ahsaie, Hamed Abedini
Summary: In this study, an aqueous two-phase system (ATPS) was used for the purification of C-phycocyanin. The Pluronic 10R5/potassium phosphate buffer system was found to be the most suitable system for separating C-phycocyanin from contaminants. Under certain conditions, the purity index of C-phycocyanin can reach 3.92 with a recovery of 90%. Additionally, using the ultrafiltration method, the purity factor can reach 5.9. Circular dichroism (CD) spectroscopy confirmed that the structure of C-phycocyanin remained intact during the purification process. Finally, 78% of the 10R5 copolymer was recovered by increasing the temperature above 57 degrees C.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Hye Min Seo, Seulwoo Kim, Sangwoo Kwon, YongJoo Kim, Minchul Sung, Jongryeol Yang, Boryeong Lee, Jongbaek Sung, Min -Ho Kang, Jungwon Park, Kyounghee Shin, Won Bo Lee, Jin Woong Kim
Summary: A smart polymer laminate film system with direction-controlled phase separation behavior was developed in this study, where liquid nanodrops were uniformly confined in a polymer laminate film through the layer-by-layer deposition of oppositely charged emulsion nanodrops and polyelectrolytes.
Article
Chemistry, Multidisciplinary
Stephan Buecker, Lutz Grossmann, Myriam Loeffler, Elena Leeb, Jochen Weiss
Summary: This study investigates the color stability of phycocyanin stabilized by lambda-carrageenan under different storage conditions. The results show that the addition of lambda-carrageenan improves the color stability of phycocyanin and soluble complexes at a pH < pI are more resistant to high treatment temperatures.
Article
Biotechnology & Applied Microbiology
Yun Ji Cho, Byung Man Lee, Youngbin Baek, Hwa Sung Shin
Summary: This study focuses on the selective extraction of chlorophyll a-pertaining photosystem polypeptides from Spirulina using aqueous two-phase extraction. The formation of a stable interfacial layer is found to be helpful in extracting the target molecules, and the purified C-Phycocyanin is proven to be safe for use.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Chemistry, Physical
Mengqi Li, Deyu Li, Yongxin Song, Dongqing Li
Summary: ATPS electrophoresis in a microfluidic chip enables separation of particles and cells through controlled transfer across the interface, offering opportunities for multidimensional particle and cell sorting in fields such as seed selection, environmental assessment, and biomedical research.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemical Research Methods
Joo Shun Tan, Sahar Abbasiliasi, Japareng Lalung, Yew Joon Tam, Paramasivam Murugan, Chee Keong Lee
Summary: This study successfully partially purified phycocyanin from Phormidium tergestinum using an ATPS system, providing a potential method for its extraction.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Vaibhav Sunil Tambat, Anil Kumar Patel, Chiu-Wen Chen, Tirath Raj, Jo-Shu Chang, Reeta Rani Singhania, Cheng-Di Dong
Summary: Globally, environmental concerns are increasing due to pollution levels, particularly hazardous heavy metals like vanadium. This study aims to develop a sustainable method for vanadium removal and utilize the biomass for biofuels, addressing the lack of green treatment methods. The use of microalgae for vanadium treatment has shown promising results, with significant improvement in removal efficiency after optimizing key parameters.
ENVIRONMENTAL POLLUTION
(2023)
Article
Agricultural Engineering
Bo Zhang, Zhixia He, Zhixiang Xu
Summary: This study investigated the interactions between liquefaction temperature and residence time of the model compound, C-PC, and optimized the operation parameters based on the experimental design. It also found that recycling the aqueous phase (AP) significantly increased the bio-oil yield and led to the transformation of organic acids from the AP to the oil phase. The proposed reaction pathway provides insights into the liquefaction process of C-PC with AP recycling.
BIOMASS & BIOENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Liang Ji, Yueling Liu, Jianquan Luo, Jianhua Fan
Summary: Microalgae are a promising alternative source for natural phycobiliprotein production, but their high production cost limits their viability. This study developed a new extraction and purification method to recover B-phycoerythrin (B-PE) from Porphyridium purpureum using a freeze-thaw-assisted aqueous two-phase system (FT-ATPS). The results showed that low light induced B-PE synthesis but hindered growth. Stepwise light supplementation and the green freeze-thaw method improved production and extraction efficiency. The purity and concentration of B-PE in the crude extract also had a significant effect on the purification process. Overall, this method can significantly reduce costs and have more environmental benefits.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Engineering, Chemical
Jennifer Sanchez-Laso, Juan J. Espada, Rosalia Rodriguez, Gemma Vicente, Luis Fernando Bautista
Summary: A new biorefinery utilizing Arthrospira platensis was developed to extract phycocyanin (PC) and produce biocrude through hydrothermal liquefaction (HTL). The use of a reusable ionic liquid [EMIM][EtSO4] allowed for the extraction of PC with high purity. Two downstream processes were used to further purify the PC, resulting in analytical grade purity suitable for pharmaceutical and nutraceutical applications. The waste biomass from PC extraction was valorized through HTL, with the addition of isopropanol leading to a significant increase in biocrude yield and composition.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Food Science & Technology
Youzhi Yu, Xiaoyu Hou, Qing Yu, Yan Huo, Kaixuan Wang, Xiaobin Wen, Yi Ding, Yeguang Li, Zhongjie Wang
Summary: By adjusting light intensity and nitrogen supply at different cultivation stages, this study aims to improve the production efficiency and extract quality of C-phycocyanin in cyanobacterium Arthrospira platensis HN5. By adopting an optimized two-stage culture strategy, up to 18.86% content and 59.72 mg L-1 d-1 productivity of C-phycocyanin were obtained. The increased C-phycocyanin content improved the quality of C-phycocyanin extract, and reactive grade C-phycocyanin was recovered through a one-step ammonium sulfate precipitation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agricultural Engineering
Adriana Ramirez-Romero, Marion Martin, Alana Boyer, Romain Bolzoni, Lucie Matricon, Jean-Francois Sassi, Jean-Philippe Steyer, Florian Delrue
Summary: Hydrothermal liquefaction (HTL) can produce bio-crude oil from wet algae, along with an aqueous phase (AP) containing minerals. However, the presence of inhibitors limits the utility of minerals in AP. Adaptive laboratory evolution can counterbalance AP toxicity and restore the fitness of microalgae.
BIORESOURCE TECHNOLOGY
(2023)
Article
Physics, Multidisciplinary
Weiming Xiong, Weijin Chen, Yue Zheng
Summary: A ferroelectric nanoplate junction is designed to control the vortex chirality by scanning local electric and thermal fields, enabling the design of vortex memory devices.
FRONTIERS IN PHYSICS
(2022)
Correction
Chemistry, Applied
Renao Bai, Peng Su, Guo Zhen, Trung T. Nguyen, Yong Diao, Kirsten Heimann, Wei Zhang
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Crystallography
Monica Bernard Tan, Alexandre N. N. Sobolev, Colin L. L. Raston, Scott J. J. Dalgarno, Irene Ling
Summary: This study examines the single crystal structures of two bulky phosphonium compounds and investigates their self-assembly behavior. It is found that the organic cations form stable stacked columns with voids occupied by anions or solvent molecules. The analysis suggests that hydrogen bonding plays a dominant role in stabilizing the self-assembly.
Article
Nanoscience & Nanotechnology
Xuan Luo, Wenjin Xing, Iliana Delcheva, Fayed Abdullah Alrashaidi, Amir Heydari, Dennis Palms, Vi Khanh Truong, Krasimir Vasilev, Zhongfan Jia, Wei Zhang, Peng Su, Kasturi Vimalanathan, Aghil Igder, Gregory A. Weiss, Youhong Tang, Melanie MacGregor, Colin L. Raston
Summary: A shear-stress-mediated platform called portable vortex-fluidic device (P-VFD) has been developed to overcome the diffusion-limit and achieve fast reaction rates. The platform consists of a plasma oxazoline-coated polyvinyl chloride (POx-PVC) film with tough hydrogel covalently bound to its surface and a reactor tube for reactions. The hydrogel arrays on the film provide a strong matrix for entrapping biomolecules and tolerate shear stress, resulting in a >6-fold increase in reaction rate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biophysics
Wanling Cai, Wei Zhang, Zuliang Chen
Summary: Metal-organic frameworks (MOFs) with high surface area and biodegradability have potential in drug release. Fe3O4 nanoparticles and MOFs composite materials, with magnetic features, were investigated for norfloxacin loading and release. The composite showed pH-sensitive properties and high release efficiency, making it suitable for targeted drug delivery. Its antibacterial activity against Escherichia coli was also tested, indicating its potential as a microbial inhibitor.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Medicinal
Trung T. T. Nguyen, Thanh Hoang, Tuyet Pham, Vi Khanh Truong, Xuan Luo, Jian Qin, Wei Zhang
Summary: Shell wastes impose burdens on the shellfish industry, but they can be used for commercial chitin production to minimize negative impacts and maximize economic value.
Article
Chemistry, Medicinal
Renao Bai, Trung T. Nguyen, Yali Zhou, Yong Diao, Wei Zhang
Summary: Arthrospira maxima is a sustainable source of proteins with diverse functionalities. The residue of spent biomass still contains proteins with potential for biopeptide production. The hydrolyzed product with the highest antioxidative activity was further fractionated and purified, and the resulting biopeptides have potential applications in food and nutraceutical products.
Article
Biochemistry & Molecular Biology
Qi Hu, Xuan Luo, Damian Tohl, Anh Tran Tam Pham, Colin Raston, Youhong Tang
Summary: This study focuses on using hydrogels embedded with aggregation-induced emission (AIE) fluorescent probe TC426 to detect HSA in real time, and the vortex fluidic device (VFD) technology is utilized as a rotation strategy to control the reaction kinetics and micromixing during measurement. The results show that the introduction of VFD could significantly accelerate its fluorescence response and effectively improve the diffusion coefficient, offering a new method for future sensing research.
Article
Chemistry, Multidisciplinary
Tuyet Pham, Tien Thanh Nguyen, Ngoc Huu Nguyen, Andrew Hayles, Wenshao Li, Duy Quang Pham, Chung Kim Nguyen, Trung Nguyen, Jitraporn Vongsvivut, Neethu Ninan, Ylias Sabri, Wei Zhang, Krasimir Vasilev, Vi Khanh Truong
Summary: This study presents a novel sustainable technology, using argon atmospheric plasma jet, to transform microalgae into bioactive coatings with excellent antibacterial activity and biological compatibility. This technology has the potential to combat wound infections and advance wound care research.
Article
Chemistry, Multidisciplinary
Dechao Chen, Muhammad Usman Zia, Fan Yang, Yuting Wang, Frederic D. L. Leusch, Nam-Trung Nguyen, Wei Zhang, Yongsheng Gao, Dongyuan Zhao, Colin L. L. Raston, Qin Li
Summary: A ligand-mediated, spatially-confined synthesis method is developed to produce high-quality carbon dots from crude bioresources. The choice of solvent plays a critical role in the formation and properties of the carbon dots. The ligand-carbon interactions and electron transfer result in the formation of uniform colloidal carbon dots with multiple emissions. These carbon dots can be easily dispersed in various solvents and fabricated into thin-film devices, making them suitable for optoelectronic applications.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Suvimol Charoensiddhi, Pawadee Methacanon, Peng Su, Wei Zhang
Summary: This study investigated the anti-skin aging potential of three Southern Australian brown seaweed extracts. The results showed that the extracts from Cystophora moniliformis and Cystophora siliquosa exhibited strong inhibitory effects on glycation activity and stimulated collagen production, suggesting their potential as skin anti-aging supplements. Further in vivo studies are warranted.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Review
Chemistry, Multidisciplinary
Clarence Chuah, Xuan Luo, Javad Tavakoli, Youhong Tang, Colin L. Raston
Summary: This article introduces the application of centrifugal and shear forces in rotary systems and devices, as well as the advantages and capabilities of the vortex fluidic device (VFD). The VFD generates high shear and large surface areas through fluidic waves, enabling micro-mixing, mass and heat transfer, and control over material structure-property relationships at the nanoscale. This technology can be used in various research and industrial applications, offering a new approach for green and innovative material transformations.
Article
Biochemistry & Molecular Biology
Shan He, Yixiao Wu, Yang Zhang, Xuan Luo, Christopher T. Gibson d Jingrong Gao, Jingrong Gao, Matt Jellicoe, Hao Wang, David J. Young, Colin L. Raston
Summary: Biodegradable cellulose films derived from kombucha, processed using a vortex fluidic device, show increased mechanical strength due to intense micromixing and high shear stress of the liquid film. The films retain their biodegradability and degrade within 5 days.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
