Article
Chemistry, Physical
Majid Jafari, Faramarz Mehrnejad, Reza Talandashti, Farahnoosh Doustdar, Mohammad Reza Vakili, Afsaneh Lavasanifar
Summary: This study simulated 15 molecular systems to characterize the interactions between PAMAM dendrimers and membranes with different cholesterol ratios, explaining the structural features and geometry of their activity against cancer cells. The results demonstrated that cholesterol content in the membranes influenced the local membrane order parameters, and the dendrimers were able to create holes in cholesterol-containing membranes through hydrogen bonds and electrostatic interactions. Additionally, negatively charged phospholipids in cancer cell membranes played a crucial role in binding with the dendrimers, providing mechanistic insight into their activity against cancer cells.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Tao Zhou, Jianlei Qian, Xin Lu, Heng Xu, Lei Chen, Chuanlei Zhang
Summary: Two flexible ligands were used to construct a three-dimensional MOF with Co(NO3)(2)·6H(2)O, which was found to have a novel topological structure through analysis. The MOF was characterized by various methods and its properties were explored.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Maria Dabkowska, Zofia Ulanczyk, Karolina Luczkowska, Dorota Roginska, Anna Sobus, Monika Wasilewska, Maria Olszewska, Katarzyna Jakubowska, Boguslaw Machalinski
Summary: The electrokinetic charge of dendrimer core influences BDNF release profile and cytotoxicity of nanoparticles. Positively charged nanoparticles core shows higher cytotoxicity and serious phenotype alterations compared to negatively charged nanoparticles core. PEG adsorption on nanoparticles with negatively charged core reduces metabolic cell activity, while charge neutralization on positively charged nanoparticles core does not reduce cytotoxicity.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Instruments & Instrumentation
Beyza B. Gokce, Tugce Boran, Filiz Emlik Calik, Gul Ozhan, Rana Sanyal, Sevgi Gungor
Summary: The study aimed to develop nanocarriers and gel formulations for topical acne treatment, with quantitative and visual confirmation of successful drug delivery to hair follicles. PAMAM dendrimers have the potential to improve drug localization in follicles and skin, reduce side effects, and enhance the efficacy of acne treatment.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Yongfa Cheng, Oi-Ming Lai, Chin-Ping Tan, Worawan Panpipat, Ling-Zhi Cheong, Cai Shen
Summary: Immobilization can improve the stability and reusability of lipases, but may also lead to conformational changes that decrease catalytic activity. In this study, grafting proline onto UIO-66 crystals for immobilization of Candida rugosa lipase showed enhanced enzyme activity and better recycling capabilities, indicating improved catalytic efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Polymer Science
Anjara Morgado, Francisco Najera, Anna Lagunas, Josep Samitier, Yolanda Vida, Ezequiel Perez-Inestrosa
Summary: The study presents a novel synthesis method for preparing slightly sterically hindered amino terminal polyamide dendrimers. By utilizing an AB(2) scaffold and specific relative positions of amino groups, robust dendritic structures were successfully constructed and thoroughly characterized, demonstrating a substantial improvement in the preparation of stable dendrimers with high yield and simplified purification procedures.
Review
Biochemistry & Molecular Biology
Rafael Leandro Fernandes Melo, Misael Bessa Sales, Viviane de Castro Bizerra, Paulo Goncalves de Sousa Junior, Antonio Luthierre Gama Cavalcante, Tiago Melo Freire, Francisco Simao Neto, Muhammad Bilal, Teofil Jesionowski, Joao Maria Soares, Pierre Basilio Almeida Fechine, Jose Cleiton Sousa dos Santos
Summary: Magnetic biocatalysts, combining magnetic properties and enzyme catalytic activity, offer easy recovery and reuse. Lipases immobilized by magnetic nanoparticles dominate research in this field. This review provides a bibliometric analysis and overview of the area, highlighting research advances and hotspots, as well as the diverse applications of magnetic biocatalysts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biophysics
Mianxing Luo, Liang Wang, Guo Chen, Jun Zhao
Summary: The orientation of the enzyme on the carrier's interface plays a crucial role in its activity. This study used magnetic nanoparticles to create microenvironments with different surface properties and studied their interaction with the lipase enzyme. The amount and activity of immobilized enzyme on different magnetic nanoparticles surfaces were investigated. The findings provide insights into the effect of surface induction on enzyme loading and activity, contributing to the rational design of immobilized enzyme methods.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Polymer Science
R. M. England, S. Sonzini, D. Buttar, K. E. Treacher, M. B. Ashford
Summary: This study characterized poly(l-lysine) dendrimers using advanced analytical techniques and molecular dynamics simulations. The results showed an increase in refractive index and intrinsic viscosity in the early generations of the dendrimers, which decreased in later generations. The protected dendrimers had different molecular density profiles compared to the unprotected dendrimers, possibly due to electrostatic repulsion. This research provides valuable insights into the structure and properties of PLL dendrimers for drug delivery applications.
Article
Chemistry, Analytical
Yin Dai, Wei Jiang, Yani Zheng, Pei He, Ruizhi Zhu, Jianwen Fei, Wei Xu, Chunbo Liu, Junli Hong
Summary: In this study, a selective method for the determination of myosmine was developed using molecularly imprinted polymers based on Fe3O4@SiO2 and PAMAM dendrimers. The MIPs showed excellent application prospects for the sensitive determination of trace myosmine in tobacco products with advantages of simple preparation and remarkable selectivity.
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Jakub Zdarta, Agnieszka Kolodziejczak-Radzimska, Karolina Bachosz, Agnieszka Rybarczyk, Muhammad Bilal, Hafiz M. N. Iqbal, Boguslaw Buszewski, Teofil Jesionowski
Summary: The combination of biotechnology and nanotechnology has led to the development of multienzyme co-immobilization as a promising approach in biocatalysis engineering. This has resulted in the creation of multipurpose biocatalysts, such as multienzyme co-immobilized constructs, to meet industrial demands. These biocatalysts offer selectivity, stability, and high catalytic turnover, making them essential tools in biocatalysis/protein engineering.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biophysics
Hongbo Suo, Moju Li, Renmin Liu, Lili Xu
Summary: In this study, imidazolium-based ionic liquid was successfully grafted to magnetic polydopamine nanoparticles for lipase immobilization. The immobilized lipase showed excellent activity and stability, with increased affinity to substrate. Additionally, the immobilized lipase can be easily separated from the reaction system with a magnet, providing new ideas for further studies in this field.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Naba Kumar Kalita, Minna Hakkarainen
Summary: A green strategy was demonstrated to accelerate the biodegradation rate of cellulose acetate (CA) through deacetylation. The immobilization of lipase from Candida rugosa on CA particles resulted in significant deacetylation, as evidenced by changes in molecular weight and water contact angle. This approach showed promising potential for developing modified lignocellulose materials with retained biodegradability.
Article
Biochemistry & Molecular Biology
Khushbu Priyadarshi, Kavita Shirsath, N. Bhargav Waghela, Anupama Sharma, Ajay Kumar, Chandramani Pathak
Summary: The study focused on improving the efficacy of anti-cancer drugs by conjugating Gallic acid with PAMAM dendrimers. This conjugate showed increased activity in inhibiting cell proliferation, restricting cancer cell migration, and inducing apoptotic cell death.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
