期刊
TOXINS
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/toxins8010014
关键词
enterohemorrhagic Escherichia coli; Shiga toxin; Bacteroides thetaiotaomicron; vitamin B-12
资金
- INRA
- PHC Sakura
- 61st Session de la Commission permanente de cooperation franco-quebecoise [61.116]
- Natural Sciences and Engineering Research Council of Canada (NSERC) [SD-25120]
- Institut de recherche en sante publique de l'Universite de Montreal [FRSQ 40148]
- CRIPA [FQRNT RS-17946]
Enterohemorrhagic Escherichia coli (EHEC) are foodborne pathogens responsible for the development of bloody diarrhea and renal failure in humans. Many environmental factors have been shown to regulate the production of Shiga toxin 2 (Stx2), the main virulence factor of EHEC. Among them, soluble factors produced by human gut microbiota and in particular, by the predominant species Bacteroides thetaiotaomicron (B. thetaiotaomicron), inhibit Stx2 gene expression. In this study, we investigated the molecular mechanisms underlying the B. thetaiotaomicron-dependent inhibition of Stx2 production by EHEC. We determined that Stx2-regulating molecules are resistant to heat treatment but do not correspond to propionate and acetate, two short-chain fatty acids produced by B. thetaiotaomicron. Moreover, screening of a B. thetaiotaomicron mutant library identified seven mutants that do not inhibit Stx2 synthesis by EHEC. One mutant has impaired production of BtuB, an outer membrane receptor for vitamin B-12. Together with restoration of Stx2 level after vitamin B-12 supplementation, these data highlight vitamin B-12 as a molecule produced by gut microbiota that modulates production of a key virulence factor of EHEC and consequently may affect the outcome of an infection.
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