期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 6, 期 1, 页码 306-315出版社
WILEY
DOI: 10.5966/sctm.2015-0426
关键词
Skin; Bone morphogenetic protein receptor type IB; Bone tissue engineering; Coral; Calvarial defect
资金
- National Natural Science Foundation of China [81272019, 30970744]
- National Institutes of Health [AR062763-01A1]
Finding appropriate seed cells for bone tissue engineering remains a significant challenge. Considering that skin is the largest organ, wehypothesized that human bone morphogenetic protein receptor type IB (BmprIB)+ dermal cells could have enhanced osteogenic capacity in the healing of critical-sized calvarial defects in an immunodeficient mouse model. In this study, immunohistochemical staining revealed that BmprIB was expressed throughout reticular dermal cells; the positive expression rate of BmprIB was 3.5% +/- 60.4% in freshly separated dermal cells, by flow cytometry. Furthermore, in vitro osteogenic capacity of BmprIB+ cells was confirmed by osteogenic-related staining and marker gene expression compared with unsorted dermal cells. In vivo osteogenic capacity was demonstrated by implantation of human BmprIB+ cell/coral constructs in the treatment of 4-mm diameter calvarial defects in an immunodeficient mouse model compared with implantation of unsorted cell/coral constructs and coral scaffold alone. These results indicate that the selective cell population BmprIB from human dermis is a promising osteogenic progenitor cell that can be a large-quantity and high-quality cell source for bone tissue engineering and regeneration.
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