Article
Plant Sciences
Thomas A. DeFalco, Pauline Anne, Sean R. James, Andrew C. Willoughby, Florian Schwanke, Oliver Johanndrees, Yasmine Genolet, Paul Derbyshire, Qian Wang, Surbhi Rana, Anne-Marie Pullen, Frank L. H. Menke, Cyril Zipfel, Christian S. Hardtke, Zachary L. Nimchuk
Summary: This study uncovers a common regulatory mechanism involving a RLCK kinase and a PP2C phosphatase that regulates receptor signaling in both immune responses and plant development.
Article
Plant Sciences
Yazhen Guo, Yabo Shi, Yalin Wang, Fang Liu, Zhen Li, Junsheng Qi, Yi Wang, Jingbo Zhang, Shuhua Yang, Yu Wang, Zhizhong Gong
Summary: In this study, a new rice PP2C phosphatase, ZmPP84, was identified as a negative regulator of drought tolerance. The study revealed that ZmPP84 inhibits stomatal closure by dephosphorylating ZmMEK1 and repressing ZmMEK1-ZmSIMK1 signaling pathway.
Article
Biochemistry & Molecular Biology
Jing Xin, Shanshan Guo, Xiaolei Zhang, Jiahui Tian, Yu Sun, Jian-Xiu Shang
Summary: Protein tyrosine phosphatases play crucial roles in plant growth, development, and stress responses, with DSP3 and DSP5 acting as negative regulators of plant salt tolerance. Interactions between DSP5 and MPK3/6 highlight a novel mechanism for salt stress regulation in Arabidopsis.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Biochemistry & Molecular Biology
Yang Liu, Tingting Gong, Xiangjiu Kong, Jiaqi Sun, Lijing Liu
Summary: Plants produce reactive oxygen species (ROS) to activate immune responses after pathogen infection. This study identified XCP1 and CYS6 as regulators of pattern-triggered immunity (PTI) and revealed their role in mediating ROS production through the degradation of RBOHD in vacuoles. These findings suggest a conserved strategy for regulating ROS production in plants.
Article
Plant Sciences
Xunlu Zhu, Guoxin Shen, Inosha Wijewardene, Yifan Cai, Nardana Esmaeili, Li Sun, Hong Zhang
Summary: Ethylene is a key plant hormone that regulates growth, development, and defense responses. The study demonstrates that PP2A-B'zeta positively regulates plant germination and seedling development, while also negatively regulating ethylene signaling pathway through interaction with CTR1. Further research is needed to fully understand the role of PP2A in ethylene responses.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Virology
Hong-Shuai Gao, Rong-Min Hu, Ze-Hua Wang, Xi-Qian Ye, Xiao-Tong Wu, Jian-Hua Huang, Zhi-Zhi Wang, Xue-Xin Chen
Summary: In this study, a PTP gene, CvBV_12-6, derived from CvBV was characterized and found to negatively regulate the immune response in the host P. xylostella. It suppressed the phenoloxidase activity and antibacterial abilities of hemocytes, as well as reduced the host's melanization and viability.
Article
Plant Sciences
Jennifer Bortlik, Jost Luehle, Saleh Alseekh, Christoph Weiste, Alisdair R. Fernie, Wolfgang Droege-Laser, Frederik Boernke
Summary: The DUF581-9 protein binds to the catalytic SNF1-related protein kinase 1 (SnRK1.1α) subunit to inhibit its activation by phosphorylation. Overexpression of DUF581-9 interferes with adaptation to starvation.
Article
Microbiology
Miaomiao Ma, Wei Wang, Yue Fei, Hang-Yuan Cheng, Beibei Song, Zhaoyang Zhou, Yan Zhao, Xiaojuan Zhang, Lin Li, She Chen, Jizong Wang, Xiangxiu Liang, Jian-Min Zhou
Summary: Plants use cell surface receptors to recognize immune patterns and activate defenses, but how these receptors regulate immune signaling in the nucleus is not well understood. In this study, the researchers discovered that the kinase BIK1 phosphorylates XLG2, promoting its nuclear translocation and positively regulating antibacterial immunity. XLG2 interacts with nuclear-localized MLKs to control transcriptome programming. MLKs negatively regulate plant immunity in a kinase activity-dependent manner, while XLG2 inhibits MLK kinase activity to promote defense gene expression and antibacterial immunity. These findings reveal a previously unknown pathway and mechanisms by which cell surface receptors regulate transcriptome during pathogen invasion.
CELL HOST & MICROBE
(2022)
Article
Biochemistry & Molecular Biology
Qiujiao Yang, Jianhang Guo, Hairuo Zeng, Lahong Xu, Jiao Xue, Shi Xiao, Jian-Feng Li
Summary: CDG1 functions as a negative regulator in Arabidopsis, negatively regulating flg22- and chitin-triggered immunity by promoting FLS2 and CERK1 degradation. Additionally, CDG1 is partially required for AvrRpm1-induced RIN4 phosphorylation.
Review
Biochemistry & Molecular Biology
Shutang Tan, Christian Luschnig, Jiri Friml
Summary: The plant hormone auxin is central to plant growth and development, with protein phosphorylation emerging as a crucial post-translational modification type regulating auxin output. This review comprehensively discusses the genetics, biochemistry, and cell biology of kinases and phosphatases involved in auxin action, providing insights into the mechanisms of developmental auxin responses and future directions for integrating protein phosphorylation control into the molecular auxin network.
Article
Biochemistry & Molecular Biology
Zengxiu Feng, Hongyong Shi, Minghui Lv, Yuang Ma, Jia Li
Summary: Protein farnesylation plays a significant role in mediating BR signaling in Arabidopsis, regulating the protein stability of the transcription factor BES1.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
Zahra Ayatollahi, Vaiva Kazanaviciute, Volodymyr Shubchynskyy, Kotryna Kvederaviciute, Manfred Schwanninger, Wilfried Rozhon, Michael Stumpe, Felix Mauch, Sebastian Bartels, Roman Ulm, Salma Balazadeh, Bernd Mueller-Roeber, Irute Meskiene, Alois Schweighofer
Summary: This study reveals the crucial role of different types of MAPK phosphatases in plant stress responses and the tight control of MAPK activities.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Yanan Liu, Hua Cheng, Peilei Cheng, Chunmeng Wang, Jiayu Li, Ye Liu, Aiping Song, Sumei Chen, Fadi Chen, Likai Wang, Jiafu Jiang
Summary: This study reveals that CmBBX22 negatively regulates drought tolerance in chrysanthemum by modulating the abscisic acid response, stomatal conductance, and antioxidant responses.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xin Qi, Wensi Tang, Weiwei Li, Zhang He, Weiya Xu, Zhijin Fan, Yongbin Zhou, Chunxiao Wang, Zhaoshi Xu, Jun Chen, Shiqin Gao, Youzhi Ma, Ming Chen
Summary: Plant G proteins play a key role in signaling transduction pathways, with AGB1 interacting with the GA pathway suppressor MYB62 to regulate plant growth. The study shows that MYB62 is downstream of AGB1 in the GA pathway, and AGB1 negatively regulates MYB62 activity by affecting its binding on the promoter of the GA degradation gene GA2ox7.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zarka Sarwar, Nusrat Nabi, Sameer Ahmed Bhat, Syed Qaaifah Gillani, Irfana Reshi, Misbah Un Nisa, Guillaume Adelmant, Jarrod A. Marto, Shaida Andrabi
Summary: In this study, we found that DBC1 interacts with PyST in mammalian cells, leading to the downregulation of DBC1 protein levels. Additionally, LKB1 can degrade DBC1 in an AMP kinase-independent manner and negatively regulates AKT1 activity through DBC1 and its downstream substrate TRB3. Furthermore, we demonstrated that DBC1 has oncogenic potential. Our findings provide insights into the LKB1-DBC1-AKT1 signaling paradigm and its role in cell cycle regulation, apoptosis, and tumorigenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Philipp Koster, Thomas A. DeFalco, Cyril Zipfel
Summary: This article discusses recent research on Ca2+ signaling in plant immunity, revealing some mysteries and deepening our understanding.
Article
Plant Sciences
Pedro Humberto Castro, Daniel Couto, Miguel Angelo Santos, Sara Freitas, Tiago Lourenco, Eva Dias, Stephanie Huguet, Jorge Marques da Silva, Rui Manuel Tavares, Eduardo Rodriguez Bejarano, Herlander Azevedo
Summary: Protein modification by sumoylation and reactive oxygen species are reciprocally regulated in Arabidopsis, with the sumoylation SIZ1 enzyme playing a critical role via control of gene expression.
Article
Biology
Jack Rhodes, Andra-Octavia Roman, Marta Bjornson, Benjamin Brandt, Paul Derbyshire, Michele Wyler, Marc W. Schmid, Frank L. H. Menke, Julia Santiago, Cyril Zipfel
Summary: This study reports on a previously uncharacterised family of plant signalling peptides, CTNIPs, and identifies the orphan receptor HSL3 as their receptor. The HSL3-CTNIP signalling module is conserved in most extant angiosperms. This discovery is important for understanding the diverse functions of plant signalling peptides and receptor-ligand co-evolution.
Article
Multidisciplinary Sciences
Qian Wang, A. Cecilia Aliaga Fandino, Moritz Graeff, Thomas A. DeFalco, Cyril Zipfel, Christian S. Hardtke
Summary: In this study, the authors demonstrate the antagonistic relationship between CLE peptide signaling pathways and phosphoinositide kinases in the regulation of auxin efflux in phloem vascular tissue. This finding provides important insights into the mechanisms of plant development and auxin regulation.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Kyle W. Bender, Cyril Zipfel
Summary: Plant receptor kinases (RKs) regulate various aspects throughout the plant life cycle by perceiving diverse ligands and activating downstream signaling. They are encoded by expanded gene families and are typically activated by co-receptor recruitment. Post-translational modifications play crucial roles in the activation and attenuation of RK-mediated signaling and RKs activate a common set of downstream signaling processes through receptor-like cytoplasmic kinases (RLCKs).
BIOCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jiashu Chu, Isabel Monte, Thomas A. DeFalco, Philipp Koster, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel
Summary: In this study, the researchers discovered that MpRBOH1 and MpPBLa, single members of the RBOH and PBL families in the liverwort Marchantia polymorpha, are essential for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1, and this phosphorylation is crucial for chitin-induced ROS production mediated by MpRBOH1.
Article
Biochemistry & Molecular Biology
Xia Yan, Bozeng Tang, Lauren S. Ryder, Dan MacLean, Vincent M. Were, Alice Bisola Eseola, Neftaly Cruz-Mireles, Weibin Ma, Andrew J. Foster, Miriam Oses-Ruiz, Nicholas J. Talbot
Summary: The rice blast fungus is a major threat to global rice production. This study provides a detailed understanding of the biology behind the invasion of plant tissues during the disease. Through transcriptional profiling, the study identifies specific gene expression changes and a repertoire of effectors critical for successful infection.
Article
Biochemistry & Molecular Biology
Hui Li, Jinlon Wang, Tung Ariel Kuan, Bozeng Tang, Li Feng, Jiuyu Wang, Zhi Cheng, Jan Sklenar, Michelle Hulin, Yufei Li, Yi Zhai, Yingnan Hou, Frank L. H. Menke, Yanli Wang, Wenbo Ma
Summary: Pathogens employ a strategy of molecular mimicry to hijack host phosphatase and diversify their effector repertoire by protein modularity. The (L)WY-LWY module in Phytophthora effectors recruits plant PP2A core enzyme to regulate host phosphoproteins. The conservation of the PP2A-interacting module but divergent C-terminal LWY units allows for functional diversity in effector-mediated manipulation of host cellular processes.
Article
Plant Sciences
Mila C. Blekemolen, Zunyong Liu, Martin Stegman, Cyril Zipfel, Libo Shan, Frank L. W. Takken
Summary: The effector Avr2 from the root-invading pathogen Fusarium oxysporum can suppress immune signaling induced by various pathogen-associated molecular patterns (PAMPs). It targets the plant kinase BIK1 to interfere with its phosphorylation, mono-ubiquitination, and subcellular localization, thereby suppressing immune activation. This study reveals BIK1 as an effector target of a root-invading vascular pathogen, highlighting its conserved role in root and shoot immunity.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Yukihisa Goto, Noriko Maki, Jan Sklenar, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel, Yasuhiro Kadota, Ken Shirasu
Summary: This study reveals a novel negative regulator, PB1CP, for RBOHD and elucidates its possible regulatory mechanisms involving the removal of phosphorylated BIK1 from RBOHD and the promotion of RBOHD endocytosis.
Article
Multidisciplinary Sciences
Mark Adero, Jaindra Nath Tripathi, Richard Oduor, Cyril Zipfel, Leena Tripathi
Summary: Transfer of the AtEFR gene can enhance resistance to BXW disease in banana.
Article
Plant Sciences
Thomas A. DeFalco, Pauline Anne, Sean R. James, Andrew C. Willoughby, Florian Schwanke, Oliver Johanndrees, Yasmine Genolet, Paul Derbyshire, Qian Wang, Surbhi Rana, Anne-Marie Pullen, Frank L. H. Menke, Cyril Zipfel, Christian S. Hardtke, Zachary L. Nimchuk
Summary: This study uncovers a common regulatory mechanism involving a RLCK kinase and a PP2C phosphatase that regulates receptor signaling in both immune responses and plant development.
