Article
Plant Sciences
F. N. Makhubu, S. M. Nkadimeng, G. Fouche, M. C. Khosa, L. J. McGaw
Summary: The study aimed to isolate and characterize nematicidal compounds from the leaves of Leonotis leonurus, with results showing potential for controlling nematodes and phytopathogens. However, it does not support the use of free-living nematodes as a model for isolating anti-parasitic compounds from plants.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Parasitology
Moise Ondua, Emmanuel Mfotie Njoya, Muna Ali Abdalla, Lyndy J. McGaw
Summary: This study aimed to evaluate the in vitro anthelmintic activity of plant extracts from 11 traditional South African plants against Haemonchus contortus. The Typha capensis acetone extract showed strong inhibitory effects on egg hatching and larval development, while two compounds isolated from T. capensis displayed antioxidant and anthelmintic activities against H. contortus. Further research is needed to confirm the efficacy of these plant extracts in vivo.
PARASITOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Changnan Wang, Bingying Wang, Taruna Pandey, Yong Long, Jianxiu Zhang, Fiona Oh, Jessica Sima, Ruyin Guo, Yun Liu, Chao Zhang, Shaeri Mukherjee, Michael Bassik, Weichun Lin, Huichao Deng, Goncalo Vale, Jeffrey McDonald, Kang Shen, Dengke K. Ma
Summary: The megaprotein LPD-3 is critical for normal phospholipid distribution and cold resilience in C. elegans.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Jogender Singh
Summary: Forward genetics is a powerful tool for unraveling molecular mechanisms of biological processes, with C. elegans being a favored model due to ease of genetic manipulation and availability of tools. Recent advancements have accelerated mutation identification, making forward genetic screens the method of choice for understanding biological functions.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Plant Sciences
Havalli Bommegowda Rashmi, Pradeep Singh Negi
Summary: This study analyzed the antioxidant and anthelmintic potential of Surinam cherry fruit at different stages of maturity. The ripe seed ethanol extract showed the highest antioxidant potential, while the mature green pulp ethanol extract exhibited the highest anthelmintic potential in Caenorhabditis elegans and Heterorhabditis indica assays. Specific phenolic compounds in the MPE extract were highly correlated with its anthelmintic activity.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Review
Multidisciplinary Sciences
Endalkachew Aklilu
Summary: Forward genetics involves identifying the gene responsible for a mutant phenotype, while reverse genetics analyzes the phenotype following disruption of a known gene. Various methods such as chemical treatment and X-rays are used to generate random mutations in organisms, and genetic variation is measured using markers across the genome.
Article
Biochemistry & Molecular Biology
Mthandazo Dube, Mohamad Saoud, Robert Rennert, Ghislain Wabo Fotso, Kerstin Andrae-Marobela, Peter Imming, Cecile Haeberli, Jennifer Keiser, Norbert Arnold
Summary: Ozoroa insignis Del. is a plant widely used in traditional medicine for various ailments. The anthelmintic principles isolated from its fruits showed good activity against parasitic organisms, with compound 1 being the most effective against newly transformed schistosomula.
Review
Plant Sciences
F. N. Makhubu, M. C. Khosa, L. J. McGaw
Summary: Research on using plants as control agents for plant-parasitic nematodes has shown that certain South African plants can effectively reduce the population density of nematodes in the soil, with in vitro experiments serving as important guides to evaluate the efficacy of plant extracts or compounds. Future research will focus on exploring the effectiveness of South African plants as control agents for root-knot nematodes, as well as investigating the chemical compounds responsible for activity and the mode of action of these plants.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Multidisciplinary Sciences
Joan Carles Puchalt, Antonio-Jose Sanchez-Salmeron, Eugenio Ivorra, Silvia Llopis, Roberto Martinez, Patricia Martorell
Summary: A new monitoring machine, SiViS, was developed for analyzing C. elegans cultures seeded with E. coli using standard Petri plates. The system utilizes active vision illumination and image-processing pipelines for motion detection, demonstrating consistent replicates in lifespan experiments and no significant differences compared to manual assays. The flexibility of SiViS allows adaptation to other C. elegans studies beyond longevity assays.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Oladayo Amed Idris, Olubunmi Abosede Wintola, Anthony Jide Afolayan
Summary: The study found that extracts of Rumex crispus have significant anthelmintic activity against Caenorhabditis elegans, indicating its potential for treating endoparasite infections. Analysis through liquid chromatography-mass spectrometry revealed various compounds in the extracts. This research suggests the existence of novel compounds in Rumex crispus that could be beneficial for livestock and human health.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Hironori Takahashi, Mako Kamiya, Minoru Kawatani, Keitaro Umezawa, Yoshiaki Ukita, Shinsuke Niwa, Toshiyuki Oda, Yasuteru Urano
Summary: By developing BODIPY-derived caged compounds that release bioactive phenol derivatives upon illumination in the yellow wavelength range, optical control of neural activity in Caenorhabditis elegans can be achieved without inducing a photophobic response or crosstalk between uncaging and simultaneous fluorescence monitoring.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Hayao Ohno, Zhirong Bao
Summary: This study reveals that Caenorhabditis elegans undergoes alternative embryogenesis in response to maternal gut microbes, resulting in changes in cell divisions, morphology, and reproduction. The miR-35 microRNA family transmits intergenerational information to regulate embryonic development. These findings challenge the assumption of an invariant cell lineage in C. elegans and provide insights into how organisms adapt to environmental changes through epigenetic control.
Article
Parasitology
Victor S. Njom, Tim Winks, Oumu Diallo, Ann Lowe, Jerzy Behnke, Mark J. Dickman, Ian Duce, Iain Johnstone, David J. Buttle
Summary: Plant-derived cysteine proteinases attack nematodes by digesting the cuticle, with DPY-7 collagen identified as a target for CPs on the cuticle of C. elegans. The process of cuticle disruption involves the degradation of structural proteins leading to the loss of integrity and eventual death of the nematode. Multiple protein targets in the cuticle may hinder the development of resistance to this new anthelmintic.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Aya C. Taki, Joseph J. Byrne, Peter R. Boag, Abdul Jabbar, Robin B. Gasser
Summary: In this study, a high throughput screening assay relying on infrared light-interference to measure the motility of Caenorhabditis elegans was established. Small molecules from a library were screened, resulting in the identification of compounds that inhibit nematode motility. Future work will evaluate the potential of these hits for development as new anthelmintic candidates.
Article
Multidisciplinary Sciences
Tatsuya Shioda, Ittetsu Takahashi, Kensuke Ikenaka, Naonobu Fujita, Tomotake Kanki, Toshihiko Oka, Hideki Mochizuki, Adam Antebi, Tamotsu Yoshimori, Shuhei Nakamura
Summary: Accumulating evidence suggests that the molecular network involving neuronal MML-1 function is crucial for regulating systemic aging. This study demonstrates that MML-1 and its heterodimer partners play a primary role in neurons to extend longevity in germlineless animals, acting via downstream cascades that are distinct from HLH-30. The MML-1-GLT-5 axis in neurons is critical for maintaining proteostasis and reducing oxidative stress in long-lived animals through autophagy and peroxidase MLT-7, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Critical Care Medicine
Seth Tigchelaar, Rishab Gupta, Casey P. Shannon, Femke Streijger, Sunita Sinha, Stephane Flibotte, Michael A. Rizzuto, John Street, Scott Paquette, Tamir Ailon, Raphaele Charest-Morin, Nicolas Dea, Charles Fisher, Marcel F. Dvorak, Sanjay Dhall, Jean-Marc Mac-Thiong, Stefan Parent, Christopher Bailey, Sean Christie, Kendall Van Keuren-Jensen, Corey Nislow, Brian K. Kwon
JOURNAL OF NEUROTRAUMA
(2019)
Article
Biochemistry & Molecular Biology
Purvi C. Trivedi, Jordan J. Bartlett, Angella Mercer, Logan Slade, Marc Surette, Andrea Ballabio, Stephane Flibotte, Bahira Hussein, Brian Rodrigues, Petra C. Kienesberger, Thomas Pulinilkunnil
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Genetics & Heredity
Frank B. Ye, Akil Hamza, Tejomayee Singh, Stephane Flibotte, Philip Hieter, Nigel J. O'Neil
Article
Biochemistry & Molecular Biology
Omar A. Itani, Xuefei Zhong, Xiaoting Tang, Barbara A. Scott, Jun Yi Yan, Stephane Flibotte, Yiting Lim, Andrew C. Hsieh, James E. Bruce, Marc Van Gilst, C. Michael Crowder
Summary: Our study reveals an unrecognized higher-order-translation regulatory mechanism in a metazoan, where ribosome biogenesis genes communicate with genes controlling tRNA abundance to match the global rate of protein synthesis with available resources.
Article
Endocrinology & Metabolism
Su Wang, Stephane Flibotte, Joan Camunas-Soler, Patrick E. MacDonald, James D. Johnson
Summary: The study found that beta cells with the rs3842753 C allele had higher INS expression and showed increased endoplasmic reticulum stress gene expression, suggesting that the inherited risk of type 1 diabetes may be associated with elevated insulin production.
CANADIAN JOURNAL OF DIABETES
(2021)
Article
Virology
Xinying Wang, Marli Vlok, Stephane Flibotte, Eric Jan
Summary: The study explores a novel viral translational mechanism, uncovering the IGR IRES sequence from ancient Northwest territories cripavirus dating back 700 years. The research demonstrates that aNCV IRES can bind to ribosomes and drive translation, supporting the historical significance of this viral translational mechanism.
Article
Biochemistry & Molecular Biology
Sara Hosseini-Farahabadi, Alireza Baradaran-Heravi, Carla Zimmerman, Kunho Choi, Stephane Flibotte, Michel Roberge
Summary: The research identified a small molecule Y-320 that enhances premature termination codon (PTC) readthrough induced by G418, increasing ribosome biogenesis and protein synthesis. Y-320 enhances PTC readthrough through autocrine chemokine signaling pathway, supporting a model where increased protein synthesis enables enhanced PTC readthrough.
Article
Genetics & Heredity
Su Wang, Victor-Felix Mautner, Ralph Buchert, Stephane Flibotte, Per Suppa, Jan M. Friedman, Manraj K. S. Heran
Summary: This study utilized MRI to characterize brain morphology in adults with NF1, finding that corpus callosum and brainstem measurements were significantly greater in NF1 patients and correlated with increased white matter volume, but no robust correlations were observed between the size of these structures and clinical or neuropsychometric assessments.
ORPHANET JOURNAL OF RARE DISEASES
(2021)
Article
Multidisciplinary Sciences
Sos Skovso, Evgeniy Panzhinskiy, Jelena Kolic, Haoning Howard Cen, Derek A. Dionne, Xiao-Qing Dai, Rohit B. Sharma, Lynda Elghazi, Cara E. Ellis, Katharine Faulkner, Stephanie A. M. Marcil, Peter Overby, Nilou Noursadeghi, Daria Hutchinson, Xiaoke Hu, Hong Li, Honey Modi, Jennifer S. Wildi, J. Diego Botezelli, Hye Lim Noh, Sujin Suk, Brian Gablaski, Austin Bautista, Ryekjang Kim, Corentin Cras-Meneur, Stephane Flibotte, Sunita Sinha, Dan S. Luciani, Corey Nislow, Elizabeth J. Rideout, Eric N. Cytrynbaum, Jason K. Kim, Ernesto Bernal-Mizrachi, Laura C. Alonso, Patrick E. MacDonald, James D. Johnson
Summary: Insulin receptor protein is present in pancreatic beta-cells, but the consequences of beta-cell insulin resistance are incompletely understood. Here the authors use a combination of mouse studies and mathematical modelling to show that loss of beta-cell insulin receptor affects male and female mice differently and can contribute to hyperinsulinemia in the context of glucose stimulation.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Abigail H. Feresten, Jaffar M. Bhat, Alex J. Yu, Richard Zapf, Hamida Safi, Vinci Au, Stephane Flibotte, Claudia Doell, Donald G. Moerman, Nancy Hawkins, Catharine H. Rankin, Harald Hutter
Summary: During nervous system development, axons must navigate complex environments to reach synaptic targets. The ccd-5 gene is found to be involved in axon navigation, particularly in the navigation of pioneer axons. Defects in follower axons are mainly caused by pioneer navigation defects. In terms of nervous system function, ccd-5 mutants have minor movement defects and reduced responsiveness to mechanosensory stimuli compared to wild type.
Article
Microbiology
Parisa Chegounian, Stephane Flibotte, Kerry Peru, John Headley, Dena McMartin, Bryne Gramlich, Vikramaditya G. Yadav
Summary: By isolating two microorganisms from oil sands tailings and analyzing their transcriptomes, this study identified the genes upregulated during the degradation of Naphthenic acid fraction compounds (NAFCs). The research also revealed the metabolic network and mechanisms used by the microbial cultures for NAFC degradation, providing new directions for engineering or evolving microorganisms for more stable and aggressive degradation.
Article
Biochemistry & Molecular Biology
Tatiana Maroilley, Stephane Flibotte, Francesca Jean, Victoria Rodrigues Alves Barbosa, Andrew Galbraith, Afiya Razia Chida, Filip Cotra, Xiao Li, Larisa Oncea, Mark Edgley, Don Moerman, Maja Tarailo-Graovac
Summary: Genetic balancers in Caenorhabditis elegans are complex variants that maintain lethal or sterile mutations in a heterozygous state by suppressing crossover events. Balancers have been widely used as an important tool in C. elegans research for decades. Traditional balancers were created by random genomic rearrangements, while the more recent CRISPR-Cas9 balancers have a pre-designed chromosomal rearrangement structure. This study provides a comprehensive resource of complex genomic variations in C. elegans and highlights the power of srWGS in studying genome complexity.
Article
Biology
Anthony J. E. Berndt, Katerina M. Othonos, Tianshun Lian, Stephane Flibotte, Mo Miao, Shamsuddin A. Bhuiyan, Raymond Y. Cho, Justin S. Fong, Seo Am Hur, Paul Pavlidis, Douglas W. Allan
Article
Neurosciences
Glenn S. Wolfe, Vivian W. Tong, Emily Povse, Daniel M. Merritt, Gregory W. Stegeman, Stephane Flibotte, Derek van der Kooy
Article
Cell Biology
Nathaniel Noblett, Zilu Wu, Zhao Hua Ding, Seungmee Park, Tony Roenspies, Stephane Flibotte, Andrew D. Chisholm, Yishi Jin, Antonio Colavita
JOURNAL OF CELL BIOLOGY
(2019)