Article
Biochemistry & Molecular Biology
Roberto Docampo, Anibal Eugenio Vercesi
Summary: This review discusses the mechanisms of mitochondrial oxidant generation and removal, and the involvement of Ca2+ in trypanosome cell death, highlighting the need for further studies on ROS generation, defense mechanisms, and mitochondrial permeability transition pore in trypanosomatids.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Carolina Prolo, Damian Estrada, Lucia Piacenza, Diego Benitez, Marcelo A. Comini, Rafael Radi, Maria Noel Alvarez
Summary: This study shows that the lack of superoxide radical produced by Nox2-deficient macrophages leads to increased susceptibility to T. cruzi infection in a murine model. Reconstitution of intraphagosomal superoxide radical formation in these macrophages restores the control of infection. The presence of Nox2-derived superoxide radical plays a crucial role in controlling early phase T. cruzi infection in a murine model of Chagas disease.
Article
Materials Science, Multidisciplinary
Sen Lin, Dahao Wang, Chang Xu, Peng Zhang, Kai Gao, Chang Liu, Xifan Mei
Summary: In this study, folic acid modified celastrol-loaded nanoparticles were developed, which can release RA symptoms and have validated anti-inflammatory effects. These nanoparticles modify the polarization of macrophages and significantly reduce inflammation levels in serum and joints.
MATERIALS & DESIGN
(2022)
Article
Microbiology
Raquel S. Negreiros, Noelia Lander, Miguel A. Chiurillo, Anibal E. Vercesi, Roberto Docampo
Summary: Pyruvate, the end product of glycolysis, is transported into the mitochondria through the mitochondrial pyruvate carriers MPC1 and MPC2. Knockout of MPC1 and MPC2 in Trypanosoma cruzi affects pyruvate-driven respiration, host cell invasion, and intracellular replication of amastigotes.
Article
Biochemistry & Molecular Biology
Sumit Das, Puja Panja, Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Subrata Adak
Summary: This study reports two members of the ChaC family of gamma-glutamyl cyclotransferases in Leishmania, showing their roles in GSH degradation and cell growth.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Parasitology
Wesley Arruda Gimenes Nantes, Filipe Martins Santos, Gabriel Carvalho de Macedo, Wanessa Texeira Gomes Barreto, Luiz Ricardo Goncalves, Marina Silva Rodrigues, Jenyfer Valesca Monteiro Chulli, Andreza Castro Rucco, William de Oliveira Assis, Grasiela Edith de Oliveira Porfirio, Carina Elisei de Oliveira, Samanta Cristina das Chagas Xavier, Heitor Miraglia Herrera, Ana Maria Jansen
Summary: The study found that urbanization can impact the richness of trypanosomatids, with the species richness potentially being influenced by the abundance of small mammals. The opossum, D. albiventris, was confirmed to be a host for trypanosomatids in urban forest fragments, with T. lainsoni demonstrating a wider host range than previously described.
PARASITOLOGY RESEARCH
(2021)
Article
Immunology
Natalia Rego, Maria Gabriela Libisch, Carlos Rovira, Juan Pablo Tosar, Carlos Robello
Summary: Chagas disease, caused by Trypanosoma cruzi, can infect various nucleated cells in mammals. This study focused on the role of microRNAs in the host-T. cruzi interaction and found that miR-146a, miR-708, and miR-1246 consistently respond to T. cruzi infection across different human cell types. The response to parasite infection was broad in macrophages, while changes in microRNAs were modest in epithelial and cardiomyocytes. These findings highlight the importance of considering microRNA changes at the cellular level and suggest miR-1246 and miR-708 as potential biomarkers for Chagas disease.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Camila Macedo Medina, Eden Ramalho Ferreira, Bruno Souza Bonifacio, Renato Arruda Mortara, Alexis Bonfim-Melo
Summary: The study revealed that Trypanosoma cruzi extracellular amastigotes invade cells by relying on the host cell actin cytoskeleton. Rac1 and Cdc42 were found to be involved in the actin-dependent internalization of EAs in RAW 264.7 macrophages, suggesting a phagocytic-like mechanism in the process.
MICROBES AND INFECTION
(2021)
Article
Chemistry, Multidisciplinary
Yuxuan Ma, Zhangwei Lu, Bin Jia, Ye Shi, Jun Dong, Shuoxing Jiang, Zhe Li
Summary: This study introduces a targeted treatment for rheumatoid arthritis (RA) using folic acid-modified triangular DNA origami nanostructures (FA-tDONs). The FA-tDONs can efficiently scavenge reactive oxygen species (ROS) and nitric oxide (NO), leading to the transition of pro-inflammatory macrophages to an anti-inflammatory phenotype. In an RA mouse model, FA-tDONs treatment effectively alleviates synovial inflammation and cartilage damage, attenuating disease progression.
Article
Biology
Chelsea U. Kidwell, Joseph R. Casalini, Soorya Pradeep, Sandra D. Scherer, Daniel Greiner, Defne Bayik, Dionysios C. Watson, Gregory S. Olson, Justin D. Lathia, Jarrod S. Johnson, Jared Rutter, Alana L. Welm, Thomas A. Zangle, Minna Roh-Johnson
Summary: Recent studies have found that mitochondrial transfer between cells can impact cellular and tissue homeostasis. While most previous research focused on the transfer of functional mitochondria to cells with damaged networks, this study reveals that dysfunctional mitochondria can also be transferred between cells with functioning networks. The transfer of dysfunctional macrophage mitochondria to cancer cells leads to an accumulation of reactive oxygen species, activating signaling pathways that promote cancer cell proliferation. These findings provide insights into how transferred mitochondria can drive sustained behavioral changes in recipient cells.
Article
Immunology
Remi Hatinguais, Arnab Pradhan, Gordon D. Brown, Alistair J. P. Brown, Adilia Warris, Elena Shekhova
Summary: This study highlights the crucial role of mitochondrial ROS (mitoROS) in macrophage antifungal responses, specifically in the regulation of cytokine secretion through RET-mediated mitoROS production. Furthermore, NOX2 is demonstrated to be essential for regulating cytokine secretion by RET-associated mitoROS in response to infection with swollen conidia.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Alberto Cornet-Gomez, Lissette Retana Moreira, Mercedes Gomez-Samblas, Antonio Osuna
Summary: American trypanosomiasis, or Chagas disease, is caused by the protozoan parasite Trypanosoma cruzi and is characterized by the presence of cardiac or gastrointestinal symptoms in a large number of patients during the chronic phase of the disease. The modulation of the immune response exerted by extracellular vesicles (EVs) secreted by the parasite and immune complexes (ICs) formed after their recognition by host IgGs and the role of IgG glycosylation in this immunomodulation were analyzed. The results showed that EVs and ICs could influence the subpopulations of peritoneal macrophages, leading to an immune response in different phases of Chagas disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Maria Augusta Dario, Cristiane Varella Lisboa, Samanta Cristina das Chagas Xavier, Paulo Sergio D'Andrea, Andre Luiz Rodrigues Roque, Ana Maria Jansen
Summary: This study investigated the transmission cycle of Trypanosoma sp. in nonflying small mammals in an area where a case of acute Chagas disease occurred. Despite the low mammalian species richness and degraded environment, a high species richness of Trypanosoma, mainly T. janseni, was observed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Subhadip Choudhuri, Lizette Rios, Juan Carlos Vazquez-Chagoyan, Nisha Jain Garg
Summary: Administering therapeutic vaccines can be a viable option for combating persistent parasitemia in Chagas disease by providing a rapid, short-term stimulation of type 1 cell-mediated immunity. Additionally, adjunct therapies could be crucial for preserving mitochondrial metabolism and cardiac muscle contractility in Chagas disease. A combined therapy approach using antigen-based vaccines and small molecules to control oxidative damage may effectively preserve cardiac structure and function in Chagas disease.
EXPERT REVIEW OF VACCINES
(2021)
Article
Biochemistry & Molecular Biology
Xiao Dan Hui, Gang Wu, Duo Han, Xi Gong, Xi Yang Wu, Shu Ze Tang, Margaret A. Brennan, Charles S. Brennan
Summary: The study found that blueberry and blackcurrant powder increased the content of phenolic compounds and the in vitro antioxidant capacity of oat bran pastes, while the total flavonoid content decreased after digestion. Extracts of digested pastes prevented macrophages from experiencing intracellular reactive oxygen species accumulation, mainly by the Keap1/Nrf2 signalling pathway, suggesting enhanced antioxidant capacity.
Article
Immunology
Daniel Cisalpino, Caio T. Fagundes, Camila B. Brito, Fernando R. Ascencao, Celso M. Queiroz-Junior, Angelica T. Vieira, Lirlandia P. Sousa, Flavio A. Amaral, Leda Q. Vieira, Jacques R. Nicoli, Mauro M. Teixeira, Danielle G. Souza
JOURNAL OF IMMUNOLOGY
(2017)
Article
Immunology
Matheus B. H. Carneiro, Eric H. Roma, Adam J. Ranson, Nicole A. Doris, Alain Debrabant, David L. Sacks, Leda Q. Vieira, Nathan C. Peters
JOURNAL OF IMMUNOLOGY
(2018)
Article
Medicine, Research & Experimental
Vanessa Ribeiro Figliuolo, Liliane Martins dos Santos, Alessandra Abalo, Hayandra Nanini, Angela Santos, Nilda M. Brittes, Claudio Bernardazzi, Heitor Siffert Pereira de Souza, Leda Quercia Vieira, Robson Coutinho-Silva, Claudia Mara Lara Melo Coutinho
Article
Dentistry, Oral Surgery & Medicine
R. R. Oliveira, W. L. F. Tavares, A. L. Reis, V. A. Silva, L. Q. Vieira, A. P. Ribeiro Sobrinho
INTERNATIONAL ENDODONTIC JOURNAL
(2018)
Article
Nutrition & Dietetics
Lais Bhering Martins, Marina Chaves de Oliveira, Zelia Menezes-Garcia, Debora Fernandes Rodrigues, Jaqueline Pereira Lana, Leda Quercia Vieira, Mauro Martins Teixeira, Adaliene Versiani Matos Ferreira
Article
Immunology
Joao F. Gomes-Neto, Rossella Sartorius, Fabio B. Canto, Thamyres S. Almeida, Andre A. Dias, Carlos-Henrique D. Barbosa, Guilherme A. Melo, Ana Carolina Oliveira, Pedro-Henrique N. Aguiar, Carlos R. Machado, Herbert L. de Matos Guedes, Marcelo F. Santiago, Alberto Nobrega, Piergiuseppe De Berardinis, Maria Bellio
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Dentistry, Oral Surgery & Medicine
J. M. Braga Diniz, M. C. Espaladori, M. E. Souza e Silva, L. C. N. Brito, L. Q. Vieira, A. P. Ribeiro Sobrinho
INTERNATIONAL ENDODONTIC JOURNAL
(2019)
Article
Parasitology
Barbara Pinheiro Mendes, Ildefonso Alves da Silva Jr, Jarina Pena DaMata, Thiago Castro-Gomes, Leda Quercia Vieira, Fatima Ribeiro-Dias, Maria Fatima Horta
Article
Dentistry, Oral Surgery & Medicine
Caroline Christine Santa-Rosa, Marcela Marcal Thebit, Kamilla Faria Maciel, Luciana Carla Neves de Brito, Leda Quercia Vieira, Antonio Paulino Ribeiro-Sobrinho
BRAZILIAN ORAL RESEARCH
(2018)
Article
Dentistry, Oral Surgery & Medicine
Marcela Carvalho Espaladori, Kamilla Faria Maciel, Luciana Carla Neves de Brito, Toshihisa Kawai, Leda Quercia Vieira, Antonio Paulino Ribeiro Sobrinho
BRAZILIAN ORAL RESEARCH
(2018)
Article
Cell Biology
Mariana Pena Ribeiro Soares, Danielle Pereira Silva, Isadora Akemi Uehara, Erivan Schnaider Ramos, Paulo Vinicius Gil Alabarse, Sandra Yasuyo Fukada, Felipe Cordero da Luz, Leda Quercia Vieira, Ana Paula Lima Oliveira, Marcelo Jose Barbosa Silva
CELL BIOLOGY INTERNATIONAL
(2019)
Article
Dentistry, Oral Surgery & Medicine
I. Bracks, M. C. Espaladori, P. Barros, L. C. N. de Brito, L. Q. Vieira, A. P. Ribeiro Sobrinho
INTERNATIONAL ENDODONTIC JOURNAL
(2019)
Editorial Material
Biophysics
Leda Quercia Vieira
BIOPHYSICAL REVIEWS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Nely Cristina Medeiros Caires, Marcela Carvalho Espaladori, Warley Luciano Fonseca Tavares, Luciana Carla Neves de Brito, Leda Quercia Vieira, Antonio Paulino Ribeiro Sobrinho
BRAZILIAN ORAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
Nely Cristina Medeiros Caires, Luciana Carla Neves de Brito, Leda Quercia Vieira, Antonio Paulino Ribeiro Sobrinho
BRAZILIAN ORAL RESEARCH
(2018)