Article
Oncology
Yasuyo Sano, Toshimi Yoshida, Min-Kyung Choo, Yanek Jimenez-Andrade, Kathryn R. Hill, Katia Georgopoulos, Jin Mo Park
Summary: Research has shown that tumor-promoting environmental stress and hemodynamic changes trigger erythropoietin production in the kidneys of mice, leading to the expansion of erythroid cell populations in the spleen. These erythroid cells exhibit specific molecular features and express genes encoding immune checkpoint molecules. Antibody-mediated erythropoietin blockade can reduce tumor growth, highlighting the potential of tumor-induced erythropoietin and erythroid cells as targets for cancer treatment.
MOLECULAR CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Emily V. Mesev, Aaron E. Lin, Emma G. Guare, Brigitte L. Heller, Florian Douam, Britt Adamson, Jared E. Toettcher, Alexander Ploss
Summary: Interferons play crucial roles in antiviral defenses, and type I and III interferon receptors exhibit differences in signaling strength and dynamics. These differences are not due to ligand-binding affinity and receptor abundance, but are determined by specific regions within the intracellular domains of the receptors.
Article
Biochemistry & Molecular Biology
Zamzam Awida, Sahar Hiram-Bab, Almog Bachar, Hussam Saed, Dan Zyc, Anton Gorodov, Nathalie Ben-Califa, Sewar Omari, Jana Omar, Liana Younis, Jennifer Ana Iden, Liad Graniewitz Visacovsky, Ida Gluzman, Tamar Liron, Bitya Raphael-Mizrahi, Albert Kolomansky, Martina Rauner, Ben Wielockx, Yankel Gabet, Drorit Neumann
Summary: This study demonstrates that EPOR in the monocytic lineage is at least partially responsible for the effect of EPO on bone loss.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Emily W. Sherman, Carmen Z. Mirabelli, Vi E. Tang, Taslima T. Khan, Kyle Leix, Andrew Kennedy, Sarah Graham, Cristen Willer, Andrew Tai, Jonathan Sexton, Christiane Wobus, Brian Emmer
Summary: This study identified genes that regulate the surface abundance of ACE2, the receptor for SARS-CoV-2, in different cell lines. The findings highlight the cell type specificity of ACE2 regulatory networks and suggest potential targets for therapeutic development.
Article
Hematology
Brian M. Dulmovits, Yuefeng Tang, Julien Papoin, Mingzhu He, Jianhua Li, Huan Yang, Meghan E. Addorisio, Lauren Kennedy, Mushran Khan, Elena Brindley, Ryan J. Ashley, Cheryl Ackert-Bicknell, John Hale, Ryo Kurita, Yukio Nakamura, Betty Diamond, Betsy J. Barnes, Olivier Hermine, Patrick G. Gallagher, Laurie A. Steiner, Jeffrey M. Lipton, Naomi Taylor, Narla Mohandas, Ulf Andersson, Yousef Al-Abed, Kevin J. Tracey, Lionel Blanc
Summary: High mobility group box-1 protein (HMGB1) interferes with EPO signaling pathways, leading to reduced expansion and increased death of EPO-sensitive erythroid precursors in patients with anemia of inflammation. This study provides important insights into the chronic phase of anemia of inflammation.
Article
Immunology
Jingjing Zhang, Jinxin Qiu, Wenyong Zhou, Jianping Cao, Xuefei Hu, Wenli Mi, Bing Su, Bin He, Ju Qiu, Lei Shen
Summary: This study identifies neuropilin-1 (Nrp1) as a tissue-specific marker of lung-resident ILC2s, which enhances their function and type 2 immune response by upregulating IL-33 receptor ST2 expression. Inhibition of Nrp1 suppresses IL-5 and IL-13 production by ILC2s and protects mice from the development of pulmonary fibrosis.
Article
Biochemistry & Molecular Biology
Clara Lazzaretti, Valentina Secco, Elia Paradiso, Samantha Sperduti, Claudia Rutz, Annika Kreuchwig, Gerd Krause, Manuela Simoni, Livio Casarini
Summary: The study focused on identifying key residues in LHCGR that are likely evolved in humans to discriminate between specific binding of LH and hCG. Mutations within the receptor's leucine reach repeat 9 and 10 were found to lead to loss of discrimination ability, resembling the modulation of Lhr-mediated signaling patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Kazuki Omata, Ikki Nomura, Akito Hirata, Yuka Yonezuka, Hiroshi Muto, Ryo Kuriki, Kirin Jimbo, Koujin Ogasa, Takashi Kato
Summary: Xenopus liver maintains erythropoietic activity from larval to adult stage. During metamorphosis, a globin switch occurs, and the whole-body mass and liver also change. We developed a method to isolate and evaluate erythroid progenitors using monoclonal ER9 antibodies and Acridine orange staining. Our findings show increased erythropoiesis in the froglet liver and demonstrate growth-dependent changes in erythropoiesis patterns in specific organs of Xenopus.
Article
Multidisciplinary Sciences
Kang Ning, Wei Zou, Peng Xu, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Steve Kleiboeker, Jianming Qiu
Summary: The study identified AXL as a protein receptor for B19V to infect human erythroid progenitor cells. Knocking down the AXL gene significantly reduced B19V internalization and replication. The interaction between B19V VP1u and the extracellular domain of AXL was confirmed, suggesting the importance of AXL as a co-receptor for B19V infection.
Article
Biochemistry & Molecular Biology
Noni T. Frankenberg, Shaun A. Mason, Glenn D. Wadley, Robyn M. Murphy
Summary: This study investigates the differences in glycogen metabolism among skeletal muscle fiber types and finds that the proportion of diffusible glycogen is lower in Type I fibers compared to individuals with type 2 diabetes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Timofey Lebedev, Elmira Vagapova, Pavel Spirin, Petr Rubtsov, Olga Astashkova, Alesya Mikheeva, Maxim Sorokin, Uliana Vladimirova, Maria Suntsova, Dmitry Konovalov, Alexander Roumiantsev, Carol Stocking, Anton Buzdin, Vladimir Prassolov
Summary: The study developed a survival prediction model for NB patients based on the expression of 48 growth factor-related genes, identified signaling pathways associated with treatment response in NB patients, and showed the protective effects of growth factors EPO and NGF against anti-NB multikinase inhibitors in vitro. Considering growth factor signaling activity benefits NB outcome prediction and tailoring therapy regimens for NB.
Article
Hematology
Rachel J. Stapley, Christopher W. Smith, Elizabeth J. Haining, Andrea Bacon, Sian Lax, Vera P. Pisareva, Andrey Pisarev, Steve P. Watson, Abdullah O. Khan, Neil Morgan
Summary: SLFN14 has been identified as an endoribonuclease that regulates protein synthesis by cleaving RNA, impacting lineage commitment in hematopoiesis. A knock-in mouse model with a SLFN14 mutation similar to that found in human patients showed distinct phenotypes, with platelet defects in humans and severe microcytic erythrocytosis in mice, highlighting the species-specific role of SLFN14 in mammalian hematopoiesis.
Article
Biochemistry & Molecular Biology
Yu Han, Yuan Zhou
Summary: In this study, cell-type-specific miRNAs were identified using multiple correspondence analysis and Gini coefficients, resulting in collections of chromatin activity-specific miRNAs in 91 cell types and expression-specific miRNAs in 124 cell types. It was found that cell-type-specific miRNAs are closely associated with disease miRNAs, providing important clues for research on cancer prognosis and other aspects. Additionally, the online tool mirCellType was constructed for dissecting the cell type composition of complex samples with miRNA expression profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Hitendra S. Solanki, Eric A. Welsh, Bin Fang, Victoria Izumi, Lancia Darville, Brandon Stone, Ryan Franzese, Sandip Chavan, Fumi Kinose, Denis Imbody, John M. Koomen, Uwe Rix, Eric B. Haura
Summary: The study reveals cell type-specific adaptive responses to KRASi, with epithelial cells compensating ERBB2/3 signaling and mesenchymal cells showing activation of FGFR or AXL signaling. These findings suggest the importance of considering cell type-specific markers and targets for patient enrichment strategies with KRASi.
CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Na Li, Qiangqiang Ge, Qiong Guo, Yuyong Tao
Summary: The Wnt pathway is a vital pathway in stem cell homeostasis and tissue regeneration, and aberrant signaling in this pathway is closely associated with cancer. Developing antibodies to block the overactivation of FZDs, the main receptors in the Wnt pathway, is a feasible option for cancer treatment. In this study, isoform-specific antibodies pF8_AC3 and sF8_AG6 were identified and shown to selectively block FZD8-mediated signaling activation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemical Research Methods
Leonard Schmiester, Yannik Schaelte, Frank T. Bergmann, Tacio Camba, Erika Dudkin, Janine Egert, Fabian Froehlich, Lara Fuhrmann, Adrian L. Hauber, Svenja Kemmer, Polina Lakrisenko, Carolin Loos, Simon Merkt, Wolfgang Mueller, Dilan Pathirana, Elba Raimundez, Lukas Refisch, Marcus Rosenblatt, Paul L. Stapor, Philipp Staedter, Dantong Wang, Franz-Georg Wieland, Julio R. Banga, Jens Timmer, Alejandro F. Villaverde, Sven Sahle, Clemens Kreutz, Jan Hasenauer, Daniel Weindl
Summary: Reproducibility and reusability of results in data-based modeling studies are essential, and PEtab provides a standardized format for specification of parameter estimation problems in systems biology. The format has been implemented by eight software tools with hundreds of users, showing great potential impact in the modeling and algorithm development community.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Immunology
Soumya R. Mohapatra, Ahmed Sadik, Suraj Sharma, Gernot Poschet, Hagen M. Gegner, Tobias V. Lanz, Philippe Lucarelli, Ursula Klingmueller, Michael Platten, Ines Heiland, Christiane A. Opitz
Summary: The liver plays a critical role in maintaining homeostatic levels of essential amino acids like tryptophan. Hypoxic conditions prompt significant rewiring of tryptophan metabolism in hepatocytes, leading to reduced expression of TDO2 and increased tryptamine production. This hypoxic adaptation may be linked to hallucinogenic responses and immunosuppressive activities, highlighting the importance of hypoxia in regulating tryptophan metabolism and potential implications for mental health.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Eva Kohnert, Clemens Kreutz, Nadine Binder, Luciana Hannibal, Gregor Gorkiewicz, Alexander Mueller, Maximilian Andreas Storz, Roman Huber, Ann-Kathrin Lederer
Summary: The study suggests that there is no significant difference in the impact of vegetarian and meat-based diets on gut microbiota. Coprococcus increased in vegan participants while decreasing in meat-eaters; Roseburia and Faecalibacterium increased in meat-eaters while decreasing in vegans. These changes may serve as markers for gut microbiota health.
Article
Infectious Diseases
Tim Litwin, Jens Timmer, Mathias Berger, Andreas Wahl-Kordon, Matthias J. Mueller, Clemens Kreutz
Summary: This study establishes an individual-based model to compare different testing strategies and finds that active testing in mental health hospitals and similar facilities can significantly reduce the number of COVID-19 outbreaks.
BMC INFECTIOUS DISEASES
(2022)
Review
Biochemistry & Molecular Biology
Lorenza A. D'Alessandro, Ursula Klingmueller, Marcel Schilling
Summary: This article investigates the response of liver cells to cytokines and growth factors, with a specific focus on the regulation of growth factor receptors on the cell surface. The study suggests that mechanistic mathematical modeling based on quantitative data can help unravel these molecular-level interactions.
BIOCHEMICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Karolin Dehlke, Linda Krause, Silvana Tyufekchieva, Anastasia Murtha-Lemekhova, Philipp Mayer, Artyom Vlasov, Ursula Klingmueller, Nikola S. Mueller, Katrin Hoffmann
Summary: This study investigates the expression of cytokines and growth factors in post-hepatectomy regeneration and finds that certain expression trajectories are associated with liver failure, morbidity, and mortality. Regression models can predict mortality following major liver resection based on preoperative cytokine and growth factor signatures. Liquid-biopsy based risk profiling can predict postoperative liver failure and mortality on the first day after surgery.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jakob Ankerhold, Sebastian Giese, Philipp Kolb, Andrea Maul-Pavicic, Reinhard E. Voll, Nathalie Goppert, Kevin Ciminski, Clemens Kreutz, Achim Lother, Ulrich Salzer, Wolfgang Bildl, Tim Welsink, Nils G. Morgenthaler, Andrea Busse Grawitz, Florian Emmerich, Daniel Steinmann, Daniela Huzly, Martin Schwemmle, Hartmut Hengel, Valeria Falcone
Summary: Severe or critical COVID-19 patients exhibit dysregulated immune response with high levels of SARS-CoV-2 specific IgG antibodies. Excessive activation of FcγRIIIA/CD16A is observed in these patients, and two independent ligands are identified as the mediators of extreme activation. Soluble circulating IgG immune complexes (sICs), similar to those found in systemic lupus erythematosus, are detected in about 80% of severe and critical COVID-19 patients.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Sajib Chakraborty, Geoffroy Andrieux, Philipp Kastl, Lorenz Adlung, Sandro Altamura, Martin E. Boehm, Luisa E. Schwarzmueller, Yomn Abdullah, Marie-Christine Wagner, Barbara Helm, Hermann-Josef Groene, Wolf D. Lehmann, Melanie Boerries, Hauke Busch, Martina U. Muckenthaler, Marcel Schilling, Ursula Klingmueller
Summary: The lack of erythropoietin receptor leads to a deficiency of hemoglobin in CFU-E cells in embryos, along with massive iron overload in the fetal liver, indicating a communication issue between the liver and placenta. Additionally, the reduction of related proteins also causes a metabolic shift. Balancing interactions between erythropoiesis, iron homeostasis regulation, and metabolic reprogramming is crucial for survival.
Article
Cell Biology
Natella I. I. Enukashvily, Nikita V. V. Ponomartsev, Avanee Ketkar, Roman Suezov, Anna V. V. Chubar, Andrey D. D. Prjibelski, Daria D. D. Shafranskaya, Sabrina Elmshaeuser, Corinna U. U. Keber, Vera N. N. Stefanova, Andrey L. L. Akopov, Ursula Klingmueller, Petra I. I. Pfefferle, Thorsten Stiewe, Matthias Lauth, Anna I. I. Brichkina
Summary: The abnormal tumor microenvironment significantly influences the response of cancer to chemo- and immuno-therapy. This study demonstrates the induction of pericentromeric satellite DNA (satDNA) transcription in cancer-associated fibroblasts (CAFs) of lung adenocarcinoma. The findings suggest that satellite long non-coding (lnc) RNAs in CAFs regulate inflammatory gene expression and can be secreted, potentially playing a role in cell-cell communication in the tumor microenvironment.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Hagen M. Gegner, Thomas Naake, Aurelien Dugourd, Torsten Mueller, Felix Czernilofsky, Georg Kliewer, Evelyn Jaeger, Barbara Helm, Nina Kunze-Rohrbach, Ursula Klingmueller, Carsten Hopf, Carsten Mueller-Tidow, Sascha Dietrich, Julio Saez-Rodriguez, Wolfgang Huber, Rudiger Hell, Gernot Poschet, Jeroen Krijgsveld
Summary: Metabolomic and proteomic analyses of human plasma and serum samples have the potential to advance our understanding of disease biology. However, pre-analytical factors such as temperature and time can impact the integrity of the samples and there is a need for standardized operating procedures to ensure reliable data.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jana Samarin, Piotr Fabrowski, Roman Kurilov, Hana Nuskova, Johanna Hummel-Eisenbeiss, Hannelore Pink, Nan Li, Vivienn Weru, Hamed Alborzinia, Umut Yildiz, Laura Grob, Minerva Taubert, Marie Czech, Michael Morgen, Christina Brandstaedter, Katja Becker, Lianghao Mao, Ashok Kumar Jayavelu, Angela Goncalves, Ulrike Uhrig, Jeanette Seiler, Yanhong Lyu, Sven Diederichs, Ursula Klingmueller, Martina Muckenthaler, Annette Kopp-Schneider, Aurelio Teleman, Aubry K. Miller, Nikolas Gunkel
Summary: A study found a set of antioxidant-capacity biomarkers (ACB) in lung cancer cell lines, which are repressed by STAT3 and STAT5A/B in sensitive cells, making them susceptible to redox-targeting and ferroptosis-inducing drugs. Low ACB expression was associated with a high level of nitric oxide instead of reactive oxygen species (ROS), which is required for high replication rates. ACB expression pattern can help stratify cancer patients for clinical trials of ROS-inducing drugs.
Review
Microbiology
Ann-Kathrin Lederer, Hannah Rasel, Eva Kohnert, Clemens Kreutz, Roman Huber, Mohamed Tarek Badr, Patricia K. E. Dellweg, Fabian Bartsch, Hauke Lang
Summary: This article provides a scoping review of the current research on the associations between gut microbiota and diagnosis, progression, and prognosis of biliary tract cancer. The findings suggest differences in gut microbiota between biliary tract cancer patients and healthy controls, but no specific species have been identified. However, due to methodological flaws in most of the studies, there is currently insufficient evidence to support the use of gut microbiota diagnostics in the management of biliary tract cancer.
Article
Gastroenterology & Hepatology
Stephanie D. Wolf, Christian Ehlting, Sophia Mueller-Dott, Gereon Poschmann, Patrick Petzsch, Tobias Lautwein, Sai Wang, Barbara Helm, Marcel Schilling, Julio Saez-Rodriguez, Mihael Vucur, Kai Stuehler, Karl Koehrer, Frank Tacke, Steven Dooley, Ursula Klingmueller, Tom Luedde, Johannes G. Bode
Summary: This study reveals the importance of different macrophage populations in the liver and their activation states. The F4/80(+)/CD11b(hi)/CD14(hi) macrophages are recruited in a CCR2-dependent manner and exhibit a distinct activation state compared to other liver macrophage populations. The primary hepatocytes can induce the same specific activation state in bone marrow-derived macrophages in vitro as observed in F4/80(+)/CD11b(hi)/CD14(hi) liver macrophages in vivo.
HEPATOLOGY COMMUNICATIONS
(2023)